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Originally published online as doi:10.2353/ajpath.2009.080819 on April 6, 2009

Published online before print April 6, 2009
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(American Journal of Pathology. 2009;174:1972-1980.)
© 2009 American Society for Investigative Pathology
DOI: 10.2353/ajpath.2009.080819

Circulating Monocytes Expressing CD31

Implications for Acute and Chronic Angiogenesis

Sun-Jin Kim, Jang-Seong Kim, John Papadopoulos, Seung Wook Kim, Marva Maya, Fahao Zhang, Junquin He, Dominic Fan, Robert Langley and Isaiah J. Fidler

From the Department of Cancer Biology, Cancer Metastasis Research Center, The University of Texas M. D. Anderson Cancer Center, Houston, Texas

To identify the roles of various circulating cells (eg, endothelial and/or stem and progenitor cells) in angiogenesis, we parabiosed a wild-type syngeneic mouse with a transgenic syngeneic green fluorescent protein mouse. Following the establishment of a common circulation between these parabionts, we investigated acute (7 to 10 days), subacute (2 to 3 weeks), and chronic (4 to 6 weeks) phases of angiogenesis in wild-type mice using wound healing, implanted gel foam fragments, and subcutaneous tumor assays, respectively. We found that under in vitro conditions, circulating murine monocytes expressed F4/80, CD31, and vascular endothelial growth factor receptor 2, but neither CD133 nor von Willebrand factor, whereas murine endothelial cells expressed CD31, vascular endothelial growth factor receptor 2, and von Willebrand factor, but neither CD133 nor F4/80. Immunofluorescence analysis revealed that green fluorescent protein-positive cells in the walls of new vessels in wounds, gel foam blocks, and tumors expressed both F4/80 and CD31, that is, macrophages. Pericytes, cells that express both CD31 and desmin, were found both in the walls of tumor-associated vessels and within tumors. Collectively, these data demonstrate that monocytes (ie, cells that express both CD31 and F4/80) may be recruited to the site of tissue injury and directly contribute to angiogenesis, reaffirming the close relationships between various cell types within the reticuloendothelial system and suggesting possible targets for anticancer treatments.






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Copyright © 2009 by the American Society for Investigative Pathology.