Published online before print July 16, 2009

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(American Journal of Pathology. 2009;175:668-684.)
© 2009 American Society for Investigative Pathology
DOI: 10.2353/ajpath.2009.081126

Apoptosis of Hippocampal Pyramidal Neurons Is Virus Independent in a Mouse Model of Acute Neurovirulent Picornavirus Infection

Eric J. Buenz^*, Brian M. Sauer^*, Reghann G. LaFrance-Corey, Chandra Deb, Aleksandar Denic^*, Christopher L. German^* and Charles L. Howe^*¶

From the Program in Molecular Neuroscience,^* the Departments of Neurology, Immunology, and Neuroscience, and the Program in Translational Immunovirology and Biodefense,^¶ Mayo Clinic College of Medicine, Rochester, Minnesota

Many viruses, including picornaviruses, have the potential to infect the central nervous system (CNS) and stimulate a neuroinflammatory immune response, especially in infants and young children. Cognitive deficits associated with CNS picornavirus infection result from injury and death of neurons that may occur due to direct viral infection or during the immune responses to virus in the brain. Previous studies have concluded that apoptosis of hippocampal neurons during picornavirus infection is a cell-autonomous event triggered by direct neuronal infection. However, these studies assessed neuron death at time points late in infection and during infections that lead to either death of the host or persistent viral infection. In contrast, many neurovirulent picornavirus infections are acute and transient, with rapid clearance of virus from the host. We provide evidence of hippocampal pathology in mice acutely infected with the Theiler’s murine encephalomyelitis picornavirus. We found that CA1 pyramidal neurons exhibited several hallmarks of apoptotic death, including caspase-3 activation, DNA fragmentation, and chromatin condensation within 72 hours of infection. Critically, we also found that many of the CA1 pyramidal neurons undergoing apoptosis were not infected with virus, indicating that neuronal cell death during acute picornavirus infection of the CNS occurs in a non–cell-autonomous manner. These observations suggest that therapeutic strategies other than antiviral interventions may be useful for neuroprotection during acute CNS picornavirus infection.

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