This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Michalopoulos, G. Articles by Pitot, H. C. Search for Related Content PubMed PubMed Citation Articles by Michalopoulos, G. Articles by Pitot, H. C.

American Journal of Pathology, Vol 85, 755-772, Copyright © 1976 by American Society for Investigative Pathology

REVIEWS

Interaction of chemical carcinogens and drug-metabolizing enzymes in primary cultures of hepatic cells from the rat

G Michalopoulos, G Sattler, C Sattler and HC Pitot

The experiments described in this paper have demonstrated that hepatocytes cultured on floating collagen membranes for periods of 10 days retain their ability to respond to the inducers of drug- metabolizing enzymes, phenobarbital and methylcholanthrene, by increases in cytochromes of the cytochrome P-450 complex. Since the regulation of these cytochromes is the rate-controlling factor in the metabolism of drugs and carcinogens in hepatocytes, such experiments indicate that hepatocytes cultured on floating collagen membranes retain those functions of the liver cell responsible for the metabolism and "activation" of carcinogenic substances. The data support this hypothesis and further indicate that this system may have potential application both in the investigation of hepatocarcinogenesis by chemicals in vitro and as a screening system for the detection of substances truly carcinogenic for the mammalian organism.