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American Journal of Pathology, Vol 91, 469-482, Copyright © 1978 by American Society for Investigative Pathology

REGULAR ARTICLES

The effect of phorbol myristate acetate on the metabolism and ultrastructure of human alveolar macrophages

JR Hoidal, JE Repine, GD Beall, FL Rasp Jr and JG White

In the present investigation we examined the influence of the surface- active agent phorbol myristate acetate (PMA) and opsonized heat-killed bacteria (HKB) on oxygen consumption, superoxide release, and glucose oxidation of human alveolar macrophages (AM). Both PMA and HKB produced a surge in oxygen consumption, superoxide release, and oxidation of 1- 14C-glucose and 6-14C-glucose by human AM. Examination of AM by electron microscopy following stimulation by these two agents demonstrated membrane ruffling, loss of microvilli, and increased vacuolization in PMA-treated cells and phagocytic vacuoles containing bacteria in HKB-treated cells. The vacuolization produced by PMA- treated AM was much less striking than the vacuolization produced in PMA-treated leukocytes. The similarity in the metabolic and some of the physical responses of AM stimulated by PMA and HKB suggest that PMA may be a useful agent for evaluating cell-membrane-related events of phagocytosis in AM.




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Copyright © 1978 by the American Society for Investigative Pathology.