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American Journal of Pathology, Vol 94, 615-622, Copyright © 1979 by American Society for Investigative Pathology

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Immunocytochemical localization of parathyroid hormone in bovine parathyroid glands and human parathyroid adenomas

JM Futrell, SI Roth, SP Su, JF Habener, GV Segre and JT Potts Jr

Light and electron microscopic localization of parathyroid hormone (PTH) in human and bovine parathyroid tissue has been achieved using an indirect peroxidase labeled antibody method. Granular deposition of the reaction product was found throughout the chief cell cytoplasm. There was no nuclear staining. At the ultrastructural level, parathyroid hormone localized by this method appeared to be largely confined to the secretory granules in the cytoplasm of cells. Mitochondria and nuclei were free of reaction product. Aggregated sacs of granular endoplasmic reticulum were minimally reactive, and Golgi apparatuses did not show reaction product.




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Copyright © 1979 by the American Society for Investigative Pathology.