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American Journal of Pathology, Vol 96, 101-119, Copyright © 1979 by American Society for Investigative Pathology

REGULAR ARTICLES

Culture of human glomerular cells

TD Oberley, PM Burkholder and MD Mills

Human glomeruli were routinely cultured in Waymouth's medium supplemented with insulin and conditioned medium. Three cell types were seen in the culture of both adult and infant kidneys, but the morphology of the glomerular cellular outgrowths depended on the age of the patient from which the kidney was obtained. Cultures of glomeruli from older individuals resulted in more "differentiated" cells, but both adult and infant glomerular cells rapidly became "dedifferentiated" as the length of time in culture increased. Outgrowths of cultured glomeruli did not contain fibroblasts or tubular cells. Finally, synthesis of basement membrane material by these cultured glomerular cells was demonstrated.




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Copyright © 1979 by the American Society for Investigative Pathology.