Published online before print April 6, 2007

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Copyright © 2007 American Society for Investigative Pathology
American Journal of Pathology, doi:10.2353/ajpath.2007.060690

Accepted for publication February 20, 2007.

Article

CCR5 Deficiency Drives Enhanced Natural Killer Cell Trafficking to and Activation within the Liver in Murine T Cell-Mediated Hepatitis

Maureen N. Ajuebor^*, Zenebech Wondimu^*, Cory M. Hogaboam, Tai Le^*, Amanda E.I. Proudfoot, and Mark G. Swain^*@

From the Gastrointestinal Research Group,* Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada; the Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan; and the Serono Pharmaceutical Research Institute, Geneva, Switzerland

^@ To whom correspondence should be addressed. E-mail: swain{at}ucalgary.ca.

	Abstract

Natural killer (NK) cells are innate immune cells that are enriched in the liver, but the processes underlying NK cell trafficking to the liver and cellular activation within the liver of patients with T cell-mediated liver diseases remain poorly defined. Concanavalin A (Con A) hepatitis is a murine model mimicking many aspects of human T cell-mediated liver diseases. Here we demonstrate that severe hepatitis in CCR5-deficient (KO) mice is associated with increased hepatic NK cell recruitment driven by enhanced hepatic production of CCL5 acting via CCR1 and by enhanced hepatic NK cell activation relative to that observed in wild-type mice after Con A administration. Furthermore, NK cell depletion ameliorated severe hepatitis in CCR5 KO mice but did not alter hepatitis in wild-type mice after Con A treatment. We propose that in the setting of CCR5 deficiency NK cells assume a profound effector role in Con A hepatitis via enhanced CCL5-CCR1 driven hepatic recruitment in addition to augmented cytokine-driven NK cell activation to produce interferon-. These results highlight the potential profound impact of altered chemokine receptor expression on the innate immune response in the setting of T cell-mediated hepatitis.

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