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Published online before print June 28, 2007
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Copyright © 2007 American Society for Investigative Pathology
American Journal of Pathology, doi:10.2353/ajpath.2007.061241

Accepted for publication April 20, 2007.

Article

Re-Expression of a Developmentally Restricted Potassium Channel in Autoimmune Demyelination. Kv1.4 Is Implicated in Oligodendroglial Proliferation

Eva Herrero-Herranz*, Luis A. Pardo*, Gertrude Bunt*, Ralf Gold{dagger}{ddagger}, Walter Stühmer*, and Ralf A. Linker{dagger}{ddagger}@

From the Max-Planck-Institute of Experimental Medicine,* Göttingen; the Department of Neurology,{dagger} St. Josef Hospital, Ruhr-University Bochum, Bochum; and the Institute for Multiple Sclerosis Research,{ddagger} University of Göttingen and Gemeinnützige Hertie-Stiftung, Göttingen, Germany

@ To whom correspondence should be addressed. E-mail: ralf.linker{at}rub.de.


  Abstract

Mechanisms of lesion repair in multiple sclerosis are incompletely understood. To some degree, remyelination can occur, associated with an increase of proliferating oligodendroglial cells. Recently, the expression of potassium channels has been implicated in the control of oligodendrocyte precursor cell proliferation in vitro. We investigated the expression of Kv1.4 potassium channels in myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis, a model of multiple sclerosis. Confocal microscopy revealed expression of Kv1.4 in AN2-positive oligodendrocyte precursor cells and premyelinating oligodendrocytes in vitro but neither in mature oligodendrocytes nor in the spinal cords of healthy adult mice. After induction of myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis, Kv1.4 immunoreactivity was detected in or around lesions already during disease onset with a peak early and a subsequent decrease in the late phase of the disease. Kv1.4 expression was confined to 2',3'-cyclic nucleotide 3'-phosphodiesterase-positive oligodendroglial cells, which were actively proliferating and ensheathed naked axons. After a demyelinating episode, the number of Kv1.4 and 2',3'-cyclic nucleotide 3'-phosphodiesterase double-positive cells was greatly reduced in ciliary neurotrophic factor knockout mice, a model with impaired lesion repair. In summary, the re-expression of an oligodendroglial potassium channel may have a functional implication on oligodendroglial cell cycle progression, thus influencing tissue repair in experimental autoimmune encephalomyelitis and multiple sclerosis.






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Copyright © 2007 by the American Society for Investigative Pathology.