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Regular Articles |
From the Department of Dermatology,*
Charité
Hospital, Humboldt University, Berlin, Germany; Department of Pathology
and Laboratory Medicine,
University of
Kentucky Medical Center, Lexington, Kentucky; Growth Factor and
Regeneration Group,
Max-Delbrück Center
for Molecular Medicine, Berlin-Buch, Germany; and Institute of
Immunology,§
Free University Berlin, Germany
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Abstract |
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 Top Abstract IntroductionMaterials and MethodsResultsDiscussionReferences |
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Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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NT-3 is a member of the NT family and has approximately 50% homology to NGF, brain-derived neurotrophic factor (BDNF), and NT-4.8,9 The biological effects of NT-3 are mediated via two types of receptors: the tyrosine kinase receptor TrkC,10 and the low-affinity NT receptor p75NTR.11 NT-3 may also bind with low affinity to TrkA and TrkB, the high-affinity receptors for NGF (TrkA) and BDNF/NT-4 (TrkB).12
NT-3 mRNA and TrkC mRNA are expressed in the epithelium of developing rat vibrissa follicles.13,14 In adult human skin, NT-3 is reportedly produced by fibroblasts,15,16 whereas TrkC immunoreactivity (IR) has been reported in the basal and granular layers of the epidermis, on HF keratinocytes, melanocytes, Pacinian corpuscles, nerve fibers, blood vessels, and sweat glands.16-19 Gene knockout and transgenic mice have shown that skin-derived NT-3 plays an essential role in the development of rapidly and slowly adapting skin mechanoreceptors associated with HFs and Merkel cells, respectively.20,21 Notably, we have recently found that NT-3 stimulates pelage HF morphogenesis in mice.6
Potential nonneurotrophic functions of NTs in the skin are mainly thought to be associated with their ability to modulate keratinocyte proliferation and apoptosis. Thus, in vitro, NGF can stimulate human epidermal keratinocyte proliferation and suppress keratinocyte apoptosis via interaction with TrkA.22,23 In situ, NGF either stimulates or inhibits murine keratinocyte proliferation, dependent on whether all HFs in the examined skin are in telogen or anagen.24
In addition to the well established antiapoptotic properties of NTs,22,23,25 NGF may also induce apoptosis via p75NTR- or even via TrkA-signaling pathways.26-28 This is interesting, given that keratinocytes of the regressing HF compartments (eg, epithelial strand or secondary hair germ) express strong p75NTR IR during catagen development in mice.29 This raises the possibility that NTs such as NT-3 are involved in the control of apoptosis-driven HF regression (catagen).
To explore the role of NT-3 and its high-affinity receptor in hair biology, we have systematically analyzed NT-3, TrkC, and p75NTR expression during all stages of HF cycling in normal C57BL/6 mice, employing enzyme-linked immunosorbent assay (ELISA), immunocytochemistry, terminal deoxynucleotidyl transferase-mediated in situ nick end labeling (TUNEL), and reverse transcription-polymerase chain reaction (RT-PCR). In addition, HF cycling in heterozygous NT-3 knockout (+/-)30 and NT-3-overexpressing mice (promoter: K14)21 was compared with that of their corresponding wild-type littermates by quantitative histomorphometry. Finally, the effects of NT-3 on spontaneous HF regression in situ were studied, using established skin organ culture techniques.24,31 These studies provide evidence that NT-3 is indeed involved in the control of catagen.
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Materials and Methods |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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C57BL/6 mice were purchased from Charles River (Sulzfeld, Germany) and housed in community cages at the animal facilities of the Charité Hospital (Virchow Campus, Berlin). NT-3-overexpressing transgenic and heterozygous NT-3 knockout (+/-) mice were housed at the animal facilities of the University of Kentucky Medical Center or the Max-Delbrück Center for Molecular Medicine, Berlin-Buch, respectively. All mice were fed water and mouse chow ad libitum.
NT-3-overexpressing transgenic mice were generated as described,21 using the keratin 14 promoter to target NT-3 overexpression to basal epidermal keratinocytes in murine skin. NT-3 knockout mice were generated using a conventional gene-targeting technique.30 Genotyping of animals was performed using slot blot analysis of isolated tail DNA as described.21 Both NT-3-overexpressing and heterozygous (+/-) knockout mice grew normally and displayed no obvious behavioral, skin, or hair growth abnormalities.
Active hair growth (anagen) was induced in the back skin of 6- to 9-week-old C57BL/6 female mice in the telogen phase of the hair cycle (as judged from their homogeneously pink back skin color) by depilation as previously described.32 All key hair cycle stages33 were studied in at least five mice per hair cycle stage: telogen (untreated skin), anagen II (3 days after depilation), anagen IV (5 days after depilation), anagen VI (12 days after depilation), and catagen (19 days after depilation). Mice were sacrificed by cervical dislocation. For immunohistochemical analysis, the neck region of back skin was harvested parallel to the vertebral line and was embedded quickly and frozen in liquid nitrogen, using a special technique for obtaining longitudinal cryosections through the HFs from one defined site.34 Corresponding back skin samples, collected for ELISA and RT-PCR analysis, were shock-frozen in liquid nitrogen and stored at -70°C until used.
RT-PCR of NT-3 and TrkC
Semiquantitative RT-PCR analysis of NT-3, TrkC, and constitutively expressed ß-actin was performed as previously described.35,36 Total RNA was isolated from full-thickness back skin samples, which included the subcutaneous panniculus carnosus muscle layer (homogenized in liquid nitrogen), using a single-step guanidine thiocyanate-phenol-chloroform method with RNAzol B (Biotech Laboratories, Inc., Houston, TX). cDNA was synthesized by reverse transcription of 3 µg total RNA, using a cDNA synthesis kit (Invitrogen, San Diego, CA). The following sets of oligonucleotide primers were used: for ß-actin, 5'-TGG AAT CCT GTG GCA TCC ATG AAA C and 5'-TAA AAC GCA GCT CAG TAA CAG TCC G-3'; for NT-3, 5'-GGT GAA CAA GGT GAT GTC CAT C and 5'-GCT GCC CAC GTA ATC CTC CA; and for TrkC, 5'-GGG TCT GTG GTG ATG GGG ATC CAC TC-3' and 5'-GCC AAG AAT GGT CCA GGT AGA TTG-3'.37,38
Amplification was performed using Taq polymerase (Life Technologies, Inc., Grand Island, NY) over 34 cycles, using an automated thermal cycler (Perkin-Elmer Corp., Norwalk, CT). Each cycle consisted of the following steps: denaturating at 94°C (1 minute), annealing at 60°C (45 seconds), and extension at 72°C (45 seconds). PCR products were analyzed by agarose gel electrophoresis and enzymatic digestion using standard methods.39 Staining was densitometrically assessed with a video scanner using Scan Pack 2.0 (Biometra, Göttingen, Germany).
Determination of Skin NT-3 Protein Content by ELISA
For protein extraction, full-thickness samples of C57BL/6 mouse back skin, dissected at the level of the subcutis just below the panniculus carnosus at distinct stages of the induced hair cycle,32,34 were pulverized in liquid nitrogen.35,36 Per 100 mg of skin, 0.5 ml lysis buffer (50 mmol/L Tris/HCl, pH 8.0, 150 mmol/L NaCl, 1 mmol/L ethylendiaminetetraacetic acid, 1 mmol/L phenylmethylsulfonyl fluoride, 5 mmol/L iodacetamid, 10 mg/ml aprotinin, 0.2% sodium dodecyl sulfate, 1% Nonidet, and 1% Triton X-100) were added and lysed in an ultrasonic bath for 10 minutes. After a 1-hour shaking at 4°C, the mixture was sonicated again in the ultrasonic bath for 10 minutes. The solution was centrifuged for 30 minutes at 14,000 x g at 4°C, and the supernatants were frozen and stored at -80°C. For quantification of NT-3 protein, a commercially available ELISA kit was used, following the manufacturer's instructions (Promega, Madison, WI).
Immunohistochemistry
Cryostat sections (8 µm) of adolescent C57BL/6 mouse skin were used for the analysis of NT-3 and TrkC IR after fixation in acetone (-20°C, 10 minutes). In addition, cryosections of skin from NT-3 knockout (-/-) and NT-3-overexpressing transgenic mice were used as negative and positive controls, respectively, for the antiserum against NT-3. For all antisera, incubation of skin sections without primary antisera and cryostat sections of embryonic brain were used as negative and positive controls, respectively. Preabsorption of primary NT-3 or TrkC antisera with 100 µg/ml of the corresponding antigenic peptides (37°C, 60 minutes) was used as an additional negative control.
Incubation steps were interspersed by four washes with Tris-buffered saline (5 minutes each). For immunovisualization of NT-3, rabbit antiserum against amino acids 139 to 158 mapping at the amino terminus of the human NT-3 precursor (identical to the corresponding mouse sequence; Santa Cruz Biotechnology, Santa Cruz, CA),40 as well as chicken antiserum against human NT-341 (Promega), were used. For the analysis of TrkC expression, rabbit antiserum to the amino acids 798 to 812 mapping at the intracellular carboxy terminal domain of TrkC protein, gp14017,18 (Santa Cruz Biotechnology), was used.
Cryostat sections were incubated with the primary antisera (1:50) overnight at room temperature, followed by an incubation with tetramethylrhodamine-isothiocyanate-conjugated F(ab)2 fragments of a goat anti-rabbit immunoglobulin G or mouse anti-chicken immunoglobulin G (Jackson ImmunoResearch Laboratories, West Grove, PA; 1:200, 30 minutes, 37°C) following previously developed basic protocols.42 Sections were then counterstained by Hoechst 33342 (Sigma Chemical Co., St. Louis, MO; 10 µg/ml in Tris-buffered saline, 30 minutes, room temperature).29
For the double immunodetection of TrkC IR on the one hand, and p75NTR IR on the other, the tyramide-amplification method was used as described before.43,44 Briefly, after blocking of endogenous peroxidase and nonspecific avidin/biotin binding, sections were incubated in TNB buffer (DuPont NEN, Boston, MA) followed by application of rat monoclonal antibody against murine p75NTR45 (Chemicon International Inc., Temecula, CA) overnight (1:1000). Then the corresponding biotinylated goat anti-rat antiserum (1:200, 30 minutes), diluted in TNB blocking buffer (DuPont NEN), was applied. The product reaction was developed with a commercial tyramide-amplification kit (DuPont NEN): sections were incubated in streptavidin-horseradish peroxidase (1:100 in TNB, 30 minutes) and washed with TNT buffer (DuPont NEN), followed by a 10-minute application of tetramethylrhodamine-isothiocyanate-tyramide (1:50 in Amplification Diluent; DuPont NEN). Then, after blocking nonspecific binding by 10% normal goat serum, sections were incubated with rabbit antiserum against TrkC (1:50, overnight, room temperature), washed in Tris-buffered saline (three times for 5 minutes each), followed by incubation with Cy2-conjugated goat anti-rabbit secondary antibody (Jackson ImmunoResearch Laboratories; 1:20, 37°C, 1 hour). Finally, sections were washed three times with Tris buffer and then counterstained by Hoechst 33342.
Double Immunodetection of TUNEL-Positive Cells and NT-3/TrkC IR
For double immunovisualization of apoptotic cells on the one hand and NT-3 or TrkC IR on the other, a previously established protocol was used.29 Briefly, TUNEL-positive cells were detected using a commercially available kit (ApopTag; Oncor, Gaithersburg, MD) with anti-digoxigenin fluorescein isothiocyanate-conjugated F(ab)2 fragments; NT-3- or TrkC IR was visualized with application of the corresponding primary antisera, described above, followed by secondary goat anti-rabbit tetramethylrhodamine-isothiocyanate-conjugated antibody. Negative and positive controls for the TUNEL staining were run as reported.46
After washing in Tris-buffered saline, all sections were mounted with Immunomount medium (Shandon, Pittsburgh, PA). Sections were examined under a Zeiss Axioscope microscope, using the appropriate excitation-emission filter systems for studying the fluorescence induced by Hoechst 33342, fluorescein isothiocyanate, or tetramethylrhodamine-isothiocyanate. Photodocumentation was done with the help of a digital image analysis system (ISIS Metasystems, Altlussheim, Germany).
Histomorphometry and Statistical Analysis
In adolescent skin, IR patterns were scrutinized by studying at least 50 HFs per mouse, and five mice were assessed per hair cycle stage. For each stage of HF cycling, the major IR patterns were recorded in previously prepared, computer-generated schematic representations of murine HF cycling, which allow a standardized, easily reproducible, and systematic comparison of different follicular IR patterns.47 For the precise identification of the defined stages of HF cycling, histochemical detection of endogenous alkaline phosphatase activity was used, because this allows visualization of dermal papilla morphology as a useful morphological marker for staging HF development and cycling.48
The percentage of HFs in different stages of growth (anagen), regression (catagen), or resting (telogen) was assessed and calculated on the basis of generally accepted morphological criteria of hair cycle classification.4,33,47-51 Only every 10th cryosection was used for analysis to exclude repetitive evaluation of the same HF, and two or three cryosections were evaluated from each animal. All together, 200 to 250 follicles in 50 to 60 microscopic fields, derived from three to five animals of distinct age, were analyzed and compared with that of a corresponding number of HFs from the appropriate, age-matched wild-type mice.
The distance between the stratum corneum and the subcutis/panniculus carnosus border was measured for assessing the skin thickness in NT-3-overexpressing, NT-3 (+/-) knockout and corresponding wild-type animals. In total, 40 to 50 such measurements were performed in 50 to 60 microscopic fields derived from three to five animals per mutant and wild-type group. All sections were analyzed at x100 or x200 magnification, and means and SEMs were calculated from pooled data. Differences were judged as significant if the P value was lower than 0.05, as determined by the independent Student's t-test for unpaired samples.
Skin Organ Culture
Punch biopsies (4 mm) were prepared under sterile conditions from adolescent C57BL/6 mouse back skin with all HFs in the late anagen VI to early catagen stage of the induced hair cycle (day 17 after depilation),51 following previously described basic organ culture protocols,24,31 with the following modifications. Per experimental group, 8 to 10 randomized skin punches, derived from the back skin of three different mice, were placed dermis down on prehydrated gelatin sponges (Gelfoam, Upjohn Co., Kalamazoo, MI) in 35-mm Petri dishes containing 5 ml Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 50 mg/ml L-glutamine, and antibiotic/antimycotic mixture (Life Technologies, Inc.). After addition of 5 to 50 ng/ml human recombinant NT-3 (Promega), organ cultures were incubated for 48 hours at 37°C in 5% CO2 and 100% humidity. After the end of incubation, all fragments were washed repeatedly in the same medium at 4°C and fixed in 4% paraformaldehyde and embedded in paraffin for routine histology and quantitative histomorphometry. The skin thickness in biopsies was assessed as described above. The percentage of HFs in defined catagen stages was calculated in 8 to 10 biopsies per group at a magnification of x400 under a Zeiss Axioscope microscope, following accepted morphological criteria.50,51
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Results |
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As an important phenomenological indicator for a possible involvement of NT-3-related signaling in hair growth control, NT-3 and TrkC gene transcription as well as total NT-3 protein content in full-thickness adolescent mouse skin were characterized by semiquantitative RT-PCR analysis and/or ELISA during the induced, highly synchronized murine hair cycle.
High steady-state levels of NT-3 transcripts, as well as of NT-3
protein (0.8 ng/mg protein), were detected in adolescent C57BL/6 murine
back skin with all HFs in telogen, whereas no TrkC transcripts were
found at this time point (Figure 1, A, D, and E)
. Hair cycle induction and the early stages of anagen development
were accompanied by a significant decline of NT-3 transcript levels in
anagen II (Figure 1D)
and of NT-3 protein level in back skin with all
HFs in anagen III to IV (Figure 1E)
. In anagen VI skin, (ie, days 8 to
12 after depilation), cutaneous steady-state levels of NT-3 mRNA
(Figure 1D)
and of NT-3 protein (Figure 1E)
had increased in parallel
and reached a maximum at the beginning of spontaneous, synchronized
catagen transformation of murine HFs on day 17
(P < 0.01, compared with day 5). In contrast,
the steady-state levels of TrkC transcripts were maximal in late anagen
(anagen VI) and significantly declined during catagen development
(Figure 1C)
. During the subsequent catagen-telogen transformation of
HFs (days 19 to 25 after depilation),50,51
the skin levels
of NT-3 and TrkC mRNA, and of NT-3 protein, further declined
significantly, compared with the values of late anagen and/or early
catagen (Figure 1)
. Taken together, these expression data suggested an
involvement of NT-3 signaling chiefly in the control of late anagen,
and in the as yet unclear mechanisms that regulate the anagen-catagen
transformation of the HF.4,5,29,47,49,51
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To correlate the NT-3 and TrkC gene and protein expression
in full-thickness skin homogenates with the intrafollicular expression
patterns of NT-3 and TrkC antigens in situ during different
stages of HF cycling, NT-3 and TrkC IR was assessed by
immunohistochemistry in the back skin of adolescent C57BL/6 mice. The
observed expression patterns are documented by representive examples in
Figure 2
and are schematically summarized
in Figure 3
. NT-3 IR patterns were tested
with two distinct antisera (see Materials and Methods), which gave
highly similar results in all staining protocols, as well as in the
positive and negative controls (not shown). For more extensive
analyses, an antiserum against amino acids 139 to 158 mapping at the
amino terminus of the human NT-3 precursor (which is fully homologous
to the corresponding mouse sequence)40
was selected,
because it provided an optimal signal/noise ratio. As an additional
control, the patterns of TrkC IR expression were compared with those
obtained with different antisera against other Trk receptors
(full-length forms of TrkA and TrkB, a truncated form of TrkB), and
substantial differences in the IR localization were noted (not shown),
attesting to both the specificity and sensitivity of our
immunohistochemical results).
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, as well as in single perifollicular longitudinal
fibers, located at the level of the HF isthmus (the so-called
follicular neural network B).52
In contrast, no follicular
TrkC IR was found in telogen skin, whereas thick subcutaneous nerve
bundles had TrkC IR (Figure 2C)
.
With progressing anagen development (anagen IV), HF-associated NT-3 IR
became visible in single cells in the HF isthmus region, in the dermal
papilla, and in perifollicular nerve fibers (Figure 2B)
. At the same
time, follicular TrkC IR became visible, yet only in the central outer
root sheath (Figure 2D)
. Further progression of anagen development
(anagen VI) associated with strong NT-3 IR was found in the
innermost layer of the outer root sheath, namely in that region of the
HF isthmus where the inner root sheath disappears and where the outer
root sheath undergoes so-called trichilemmal
keratinization53
(Figure 2E)
. This expression
pattern of NT-3 in the upper outer root sheath remained constant
throughout the anagen-catagen transformation of the HF (Figure 3)
.
Also, a less intense NT-3 IR was seen in the bulge (Figure 2E)
, one
major location of epithelial stem cells.54
Interestingly,
no TrkC IR expression was seen in this region (Figure 2F)
. In anagen
VI, NT-3 IR was widely expressed by proximal outer root sheath
keratinocytes (Figure 2G)
, whereas many hair matrix and outer root
sheath keratinocytes displayed TrkC IR (Figure 2I)
. Prominent IR not
only for NT-3, but also for TrkC, appeared in the dermal papilla of
anagen VI HFs (Figure 2, G and I)
.
The earliest stages of HF regression (catagen I to II) were
characterized by a disappearance of TrkC IR from dermal papilla
fibroblasts, whereas some of them retained NT-3 IR (not shown). Both
antigens were strongly expressed in the proximal outer root sheath and
hair matrix keratinocytes of catagen II follicles (Figure 2, H and J)
.
With progressing HF regression, ie, during catagen V to VI, very high
levels of NT-3 and TrkC IR became visible in keratinocytes of the
regressing epithelial strand and the hair germ (Figure 2, K and L)
.
TrkC IR was also seen in the regressing inner root sheath (Figure 3
and
Figure 4, F and G
). Prominent expression of both antigens was also
observed in secondary hair germ keratinocytes in catagen VII to VIII
follicles. Individual cells in the regressing epithelial strand and the
secondary hair germ, located next to the newly developing club hair,
showed exceptionally strong NT-3 and/or TrkC IR (Figure 2, K and L)
.
During the entire hair cycle, the arrector pili muscle (Figure 2E)
and
the subcutaneous panniculus carnosus muscle showed strong NT-3 IR,
which was contrasted by an absence of TrkC IR (Figure 2, H and J)
.
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Based on the observations that the cutaneous steady-state levels
of NT-3 and TrkC gene and protein expression were up-regulated during
the anagen-catagen transformation (Figure 1)
and that NT-3 and TrkC IR
were expressed particularly strongly in regressing HF compartments
during catagen (Figures 2 and 3)
, it was logical to ask whether or not
keratinocytes undergoing catagen-associated apoptosis in the hair
matrix, epithelial strand, and secondary hair germ29
co-express NT-3 or TrkC IR.
Using a technique for double immunovisualization of TUNEL-positive and
NT-3 or TrkC IR cells in the regressing HF, we observed that, during
the entire catagen stage, the innermost layer of the outer root sheath
expressed strong NT-3 IR in approximately the region where the inner
root sheath disappears and where the outer root sheath undergoes
trichilemmal keratinization53
(Figure 4A)
. Isolated NT-3 IR keratinocytes of
this compartment also displayed TUNEL+ nuclei (Figure 4A)
, suggesting
that they had entered into the final stage of apoptosis. In the
proximal epithelium of catagen IV to V HFs, single TUNEL+ cells in the
hair matrix also showed cytoplasmic NT-3 IR (Figure 4B)
. In catagen VI,
many TUNEL+ cells and apoptotic bodies located in the epithelial strand
were also NT-3 IR (Figure 4C)
. In catagen VI to VII, some TUNEL+ cells
of the secondary hair germ and even of the most proximal club hair also
displayed NT-3 IR. However, some clearly NT-3 IR cells located in this
compartment were TUNEL negative, and vice versa (Figure 4D)
.
In contrast to the NT-3 IR patterns described above (Figure 2E)
, no
TrkC or p75NTR IR expression was observed in that part of the HF
isthmus where the innermost outer root sheath undergoes trichilemmal
keratinization (not shown). However, in the proximal epithelium of
catagen VI HFs, single TrkC IR keratinocytes in the regressing
epithelial strand were also TUNEL positive (Figure 4E)
. Later on, ie,
in catagen VI-VII, many TUNEL positive cells of the proximal club hair
showed prominent TrkC IR (Figure 4F)
. TrkC IR cells in the secondary
hair germ often co-expressed p75NTR IR (Figure 4G)
, which we had
previously shown to be up-regulated during catagen
development.29
These co-localization patterns of NT-3 and/or TrkC IR with markers associated with apoptosis (TUNEL and p75NTR)29 in cells of the regressing hair bulb suggested that NT-3 may be involved in the control of catagen and its associated keratinocyte apoptosis.29 In view of recent reports that NTs can both suppress and induce apoptosis in different model systems,27 we further explored the functional role of NT-3 in catagen control, studying appropriate mouse mutants, and the effect of NT-3 in murine skin organ culture.
NT-3 Overexpression Causes Precocious Catagen Development
To define whether constitutive NT-3 overexpression alters spontaneous catagen development, neonatal transgenic mice with intraepithelial NT-3 overexpression were compared with age-matched wild-type mice for the onset and speed of spontaneous HF regression. NT-3-overexpressing mice were generated using the K14 promoter,21 which can serve as a particularly attractive model for studying the influence of NT-3 on the anagen-catagen transformation, because, in addition to the basal layer epidermal keratinocytes, K14 targets NT-3 expression to outer root sheath keratinocyte populations and to keratinocytes of the epithelial strand, secondary hair germ, and the base of the club hair (during catagen).55,56
After the completion of HF morphogenesis (which is often mislabeled as
the "first hair cycle"), the HF begins its lifelong cycle of
regression, resting, and growth by spontaneous entry into the first
catagen stage.4,5,29
In normally cycling mice, this occurs
around postnatal day (P) 17. Therefore, exclusively anagen VI HFs were
found in the skin of wild-type controls at P14 (Figure 5A)
. In striking contrast, already 30 to
40% of HFs in the skin of NT-3-overexpressing mice displayed the
morphological characteristics of catagen II follicles at this time
(Figure 5B)
. By P17, wild-type control mice still showed no HFs in late
catagen (stages VII to VIII) (Figure 5C)
, whereas the HFs of
NT-3-overexpressing mice were already almost all in late catagen stages
(VI to VIII) (Figures 5D and 6A)
.
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). Because murine skin
thickness is strictly coupled to synchronized HF cycling, and because
catagen and telogen skin is much thinner than anagen
skin,32,33,57
this also indicated that the catagen-telogen
HF transition in NT-3-overexpressing mice had almost been completed at
this time point. At P20, only telogen HFs were found in the skin of
NT-3-overexpressing mice, whereas 25 to 30% of follicles in wild-type
mice were still in catagen VII to VIII (not shown). This strongly
suggests that intraepithelial NT-3 overexpression accelerates the
initiation and/or development of catagen. Reduced NT-3 Expression Is Associated with Catagen Retardation
Because mutant mice with a complete functional deletion of NT-3 gene are not viable and die shortly after birth,30 heterozygous NT-3 knockout (+/-) mice were used as a model for studying HF regression under reduced levels of NT-3 expression. Reportedly, these mice display a reduction of NT-3 mRNA levels in brain and kidney by about 50%.20
Heterozygous NT-3 knockout (+/-) mice displayed inverse dynamics of
catagen development compared with NT-3 transgenic mice: a significant
increase in the percentage of HFs in catagen VI to VII and a
substantial decline in that of HFs in catagen VIII-telogen was
found at P19, compared with corresponding wild-type (+/+) littermates
(Figure 6B)
. Also, skin in heterozygous NT-3 knockout mice was
substantially thicker (435.7 ± 20.3 µm) than in wild-type
animals (302.5 ± 11.6 µm, P < 0.001). This
further indicated that a reduced cutaneous NT-3 expression is
associated with catagen retardation.
NT-3 Accelerates Catagen Development in Murine Skin Organ Culture
Theoretically, alterations in HF cycling observed in NT-3 mouse mutants might be connected to the consequences of NT-3 overexpression or partial deletion, such as NT-3-dependent differences in the skin and HF innervation, as well as in the number and activity of Merkel cells,20,21,30 rather than to direct effects of NT-3 on the HF. Therefore, the action of NT-3 on the follicular anagen-catagen transformation was, finally, tested in murine skin organ culture in the absence of functional skin nerves. This assay was designed to further probe the concept that NT-3 is indeed one of the few catagen-promoting agents identified so far.4,5,47,58 NT-3 protein was added to organ-cultured murine skin with most HFs in the process of initiating the anagen VI-catagen transformation of the hair cycle. For this purpose, biopsies were taken from normally cycling C57BL/6 mouse skin 17 days after anagen induction by depilation32 to be cultured at the air-liquid interphase on gelatin gels31 for 48 hours in the presence or absence of NT-3 doses routinely used in NT studies (5 to 50 ng/ml).16,23-25
Quantitative histomorphometry revealed that NT-3 did indeed accelerate
catagen development in situ. There was a significant
increase in the number of HFs in catagen III and an absence of HFs in
late anagen VI-catagen I in those skin biopsies that had been cultured
in the presence of 5 or 50 ng/ml of NT-3 (compared with vehicle
controls, in which catagen II HFs dominated) (Figure 7A)
. As illustrated by the representative
photomicrographs shown in Figure 7, B and C
, this acceleration of
catagen development by one stage corresponded to a significant,
morphologically easily recognizable difference in skin thickness
between NT-3-treated (342.0 ± 8.6 µm) and control biopsies
(408.0 ± 9.3 µm, P < 0.001). This further
attested to the catagen-promoting activity of NT-3 in vitro.
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Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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Furthermore, we here identify NT-3 as one of the factors in the growing
list of putative catagen control signals, such as fibroblast growth
factor 5, insulin-like growth factor-1, transforming growth
factor-ß, and PTHrp (compare 4, 5,
and 58): NT-3
expression is highest during the initiation of apotosis-driven HF
regression (Figure 1)
, NT-3 and TrkC expression is up-regulated in the
regressing hair bulb (Figures 2 and 3)
, and both NT-3- and TrkC IR are
co-expressed by TUNEL+ keratinocytes of the regressing HF bulb (Figure 4)
. In addition, NT-3 overexpression is associated with accelerated
catagen development, whereas a reduction in the constitutive NT-3
expression causes catagen retardation (Figures 5 and 6)
. Finally, NT-3
administration accelerates catagen development in murine skin organ
culture even in the absence of functional skin nerves (Figure 7)
.
Compared with other NTs, the level of NT-3 protein found in adolescent
C57BL/6 mouse skin (Figure 1)
are surprisingly high, if one considers
that, at this time, the development of cutaneous innervation has
already been finalized.59
Our observations in mouse skin
are in line with a report that the levels of NT-3 protein in
nonneuronal tissues and internal organs of rats are actually higher
than in brain,60
suggesting additional, nonneuronal
functions of NT-3 in those organs during postnatal life. Skeletal
muscle is known to express high levels of NT-3 during embryonic and
postnatal ontogenesis, which may be required for the development and
function of motor neurons, muscle spindles, and Golgi tendon
organs.30,61
Therefore, the panniculus carnosus muscle in
murine subcutis may well be a major source of the high basal level of
NT-3 protein in adolescent C57BL/6 mouse skin (note the high NT-3 IR of
this muscle layer depicted in Figure 2H
). Although additional in
situ hybridization evidence is required to show that this
subcutaneous striated muscle layer really is a major NT-3 source in
normal rodent skin, our study encourages one to further explore the
role of NT-3 in the biology of mature myocytes long after the
completion of myogenesis.
Our data suggest that, besides the subcutaneous muscle layer and the
piloneural complex (Figure 2, A and B)
, the HF itself is another key
NT-3 source in murine skin. Follicular NT-3 expression showed striking
hair cycle-dependent differences, NT-3 IR was more widely distributed
in the anagen and catagen HFs compared with the telogen stage. Namely,
in anagen VI HFs, NT-3 IR was found in that part of the innermost layer
of the outer root sheath that shows trichilemmal keratinization (Figure 2E)
. This peculiar follicular keratinization pattern is characterized
by intricate infoldings of nonkeratinized and keratinized cells with
specific patterns of intracellular filament organization and keratin
expression.53,62,63
Because keratinocytes in this area
undergo apoptosis (in guinea pig HFs),64
apoptosis may be a
necessary component for the occurrence of trichilemmal keratinization.
Using TUNEL staining as a marker of apoptotic cells,23,29
our study reveals that some TUNEL+ keratinocytes of the innermost outer
root sheath also express NT-3 IR (Figure 4A)
. Although TUNEL positivity
does not always reflect apoptosis,29,65-67
this raises the
possibility that NT-3 is involved in the modulation of epithelial cell
apoptosis in this HF compartment. However, it remains to be formally
excluded by immunoelectron microscopy that TUNEL+/NT-3+ cells are in
the final stage of terminal differentiation, rather than in apoptosis.
NT-3 is strongly expressed by selected populations of HF keratinocytes
as well as by dermal papilla fibroblasts during anagen and catagen
(Figures 2 and 3)
, whereas cutaneous NT-3 gene transcription is
up-regulated during this time (Figure 1)
. Therefore, it is reasonable
to speculate that follicular NT-3 expression is tightly coupled to the
cyclic growth and regression activity of the HF. The highest levels of
NT-3 gene expression and NT-3 protein content are found during early
catagen (Figure 1)
. Together with our finding that infantile NT-3
transgenic mice show precocious development of their first entry into
catagen (Figures 5 and 6A)
, whereas catagen development is retarded in
mice with substantially diminished NT-3 skin levels (Figure 6B)
, this
strongly suggests that NT-3 stimulates the anagen-catagen-telogen
transformation of the HF. This is supported by direct proof that NT-3
promotes catagen development in murine skin organ culture (Figure 7)
.
Spontaneous catagen development is a tightly coordinated and stringently controlled process, which is thought to include a termination of growth factor production by dermal papilla fibroblasts, leading to a sudden decline in hair matrix keratinocyte proliferation and hair shaft production,4,5,68 to the induction of keratinocyte apoptosis in selected HF compartments,4,29,64 and to active club hair formation with its special type of trichilemmal keratinization that prevents hair loss during telogen.53,62,69 Obviously, NT-3-mediated stimulation of HF regression might be achieved via any of these mechanisms, in addition to the up-regulation of other, as yet unknown catagen-promoting parameters by NT-3.4
Given that, as we demonstrate here, dermal papilla fibroblasts express
TrkC receptor only in late anagen (Figure 2I)
, NT-3 may directly
stimulate TrkC-expressing dermal papilla fibroblasts, the accepted key
mesenchymal control element of HF cycling.4,5,68
However,
NT-3 can also bind with low affinity to TrkB
receptors,12,70
which are expressed by dermal papilla
fibroblasts during early catagen (Botchkarev VA, Botchkareva N, and
Paus R, unpublished observation). This raises the possibility that
NT-3/TrkB signaling may also contribute to promoting the dermal papilla
transformation from an anagen-supporting state to its catagen/telogen
state.
It can currently not be excluded that any of the proposed effects of NT-3/TrkC signaling on keratinocyte apoptosis and/or terminal differentiation primarily reflect a stimulation of the elusive molecular mechanisms that control catagen induction and, thus, indirectly catagen-associated keratinocyte apoptosis and differentiation. In our present, very limited state of understanding of the controls of catagen development,4,5 it is probably wisest to operate with the working hypothesis that NT-3/TrkC signaling is just one important element in the intrinsic "clock" that determines the switch-on of catagen as the net result of multiple catagen-promoting as well as catagen-suppressing factors (for a detailed discussion, see Ref. 4 ).
However, our data encourage one to consider that NT-3 is, at least in
part, directly involved in the regulation of keratinocyte apoptosis
during catagen: in catagen IV HFs, in which there is maximal apoptosis
in hair matrix keratinocytes,29
NT-3 IR is found in the
cytoplasm of many TUNEL+ keratinocytes (Figure 4B)
, and numerous TUNEL+
apoptotic cells and apoptotic bodies located in the epithelial strand
and secondary germ during catagen VI to VII had NT-3 IR (Figure 4, C and D)
. In other models, eg, dorsal root ganglion neurons, apoptotic
cells produce BDNF before their death to rescue bystander cells in
their direct vicinity, thus promoting the survival of BDNF-dependent
neuronal populations.71
Therefore, before their final
apoptotic death, selected keratinocytes in the regressing hair bulb
might produce NT-3 to provide a high local concentration of NT-3 for
selected, TrkC-expressing HF cell populations (Figures 2L and 4, E and
F)
designated to survive for future use during the subsequent hair
cycle.
Our data also indicate that during late catagen, some TrkC+
keratinocytes display intracellular TUNEL+ granules (Figure 4E)
or even
TUNEL+ nuclei (Figure 4F)
. This strongly suggests that there is also a
subpopulation of TrkC+ HF cells in the proximal club hair and in the
epithelial strand, which is induced to switch on apoptosis by NT-3
stimulation. This speculation is encouraged by recent data indicating
that another NT, NGF, besides its well established antiapoptotic
properties,9,12
can also induce apoptosis in selected model
systems (compare 26-28
). In addition, there appears to be yet
another subpopulation of TrkC+ keratinocytes in the secondary hair germ
of catagen VI HFs, which co-expresses p75NTR and TrkC (Figure 4G)
.
These cells might represent HF keratinocytes selected for survival,
given that the co-stimulation of p75NTR and Trk receptors reportedly
promotes the antiapoptotic effects of NTs.27
Because NT-3 is strongly expressed by keratinocytes of the secondary
hair germ, and because some of these keratinocytes also show a TUNEL+
nucleus during catagen VI to VII (Figure 4D)
, it is reasonable to ask
whether NT-3 is also involved in the complex regulation of club hair
formation,5,54
of which trichilemmal keratinization is
considered an important component.53,62,69
This unique
process of keratinocyte terminal differentiation in the outer root
sheath is associated with the synthesis of specific keratins, special
expression patterns of adhesion molecules, and
apoptosis.29,53,55,62-64,72,73
Because several cells in
the identified zones of trichilemmal keratinization (anagen: innermost
outer root sheath in the HF isthmus region; catagen and telogen:
proximal club hair) NT-3/TUNEL double positive (Figure 4, A and D)
,
this suggests that NT-3/TrkC signaling may be an important element in
the control of keratinocyte terminal differentiation and/or apoptosis
during normal club hair formation and trichilemmal keratinization in
murine HF.
Finally, given that abnormalities in catagen development are a key element in the pathogenesis of most human hair growth disorders,4 TrkC agonists and antagonists deserve to be systematically explored for the management of hair growth disorders, in which premature catagen entry of scalp HF causes effluvium and/or alopecia, or where retarded catagen development leads to excessive hair growth (hirsutism/hypertrichosis). Thus, there are both biologically and clinically compelling reasons to make NTs of the NGF family and their receptors a new focus of basic and applied hair research.
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Acknowledgements |
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Footnotes |
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Supported by grants from Deutsche Forschungsgemeinschaft (Pa 345/6-1) and Wella AG, Darmstadt (to RP), and National Institute of Neurological Diseases and Stroke grant Rol NS 33730 (to KMA).
Accepted for publication June 10, 1998.
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References |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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B by nerve growth factor through the neurotrophin receptor p75. Science 1996, 272:542-545[Abstract]
-delta T cells in murine skin during the induced hair cycle. Br J Dermatol 1994, 130:281-289[Medline]
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