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Commentary |
From the Vascular Research Division, Department of Pathology, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts
Intimal lipid accumulation, hyperplasia, and scarring are stigmata of atherosclerotic vascular disease, whose major complications—myocardial and cerebral ischemia and infarction—continue to be major health problems in developed nations.1 This insidiously progressive disease typically spans decades, but can reach a clinical horizon in a matter of minutes due to critical changes in a given atherosclerotic plaque that result in localized but life-threatening thrombosis. Epidemiological studies have established that hypercholesterolemia is an important risk factor in this disease process, and lipid-lowering drugs have been proven to have clinical efficacy. Experimental animals that are fed lipid-rich diets to elevate their plasma cholesterol levels also can develop atherosclerotic-like lesions, as do animals with naturally occurring or genetically engineered mutations that result in altered cholesterol metabolism. However, regardless of a given patient's risk factor profile, species of animal model, or type of natural or engineered genetic alteration, the early, lipid-rich lesions of atherosclerosis show a markedly nonrandom pattern of distribution within the arterial vasculature. Atherosclerotic lesions typically develop in the vicinity of branch points and areas of major curvature. These arterial geometries are associated with blood flow disturbances such as nonuniform laminar flow with boundary layer separation, complex secondary flows with flow reversal and dynamic stagnation points, and resultant temporal and spatial gradients in wall shear stresses. In contrast to these atherosclerosis-prone areas, unbranched, tubular arterial geometries, which are associated with a more uniformly laminar flow profile, characteristically are relatively atherosclerosis-resistant, at least in the early phases of the disease. This strikingly localized pattern of lesion formation, even in the face of systemic risk factors such as elevated plasma cholesterol, has intrigued experimental pathologists and fluid mechanical engineers alike for decades, and has motivated the search for a mechanistic link between hemodynamic forces and atherogenesis.
In this issue of The American Journal of Pathology, Zand and coworkers2 describe a novel experimental model system for creating flow disturbances in the aorta of the rat that have significant effects on the pattern of intimal lipid deposition induced by chronic dietary hypercholesterolemia. Surgical insertion of a hemispherical glass plug into the aortic lumen, through the ostium of a severed renal artery, created a significant stenosis (greater than 50% cross-sectional area reduction) without causing any compression of the adjacent aortic wall, as typically occurs in other models involving an externally applied ligature or metal clip. This model thus accomplishes two useful things: it reliably creates an altered vascular geometry designed to induce well characterized intraluminal flow perturbations while it minimizes the confounding issue of concomitant (and often less well characterized) changes in intramural stresses and strains. Detailed quantitative studies of the flow field perturbations created by the hemispherical plug were performed in parallel, using a scaled-up in vitro biophysical model. Histopathological analysis of the experimental aortas showed a clear-cut association of intimal lipid deposition with certain types of flow perturbation. Crescentic areas of intimal lipid accumulation and subendothelial thickening were localized both proximal and especially distal to the plug, in the regions of predicted low wall shear stress, flow separation, stagnation, and recirculation. In contrast, there were essentially no lipid deposits visible in the intimal area opposite the plug, ie, in a region of increased wall shear stress. These observations, in particular the asymmetric expansion of intimal changes in the direction of flow, also may have implications for the growth of raised atherosclerotic lesions. As the authors point out, an association of intimal lipid deposition with disturbed flow has been inferred previously by others studying the focal nature of atherosclerotic lesions in various experimental animals and human subjects. However, the current study is perhaps the clearest demonstration, to date, of this phenomenon, experimentally generated in a biophysically well characterized in vivo model.
What are the implications of these observations for the pathogenesis of atherosclerosis? In particular, what mechanisms at the cellular and molecular level link hemodynamic forces with vessel wall pathophysiology? As early as 1969, Caro and coworkers3 called attention to the association of low shear areas with early atherosclerotic lesions in human subjects. This led to the hypothesis that low (time-averaged) wall shear stresses, such as occur near the carotid bifurcation and other atherosclerosis-prone geometries, might result in prolonged resident times for large, atherogenic lipid particles (eg, low-density lipoproteins) or even blood cells (eg, platelets, mononuclear leukocytes), thus favoring their localized attachment and infiltration into the arterial wall. This reasoning also can be extended to the effect of low wall shear on local boundary-layer, mass transport-dependent processes, resulting in the localized accumulation of cytokines, growth factors, or reactive oxygen species in the vicinity of the intimal surface. This working concept invokes rheological issues per se as the critical determinants and views the contribution of the vessel wall as relatively passive. In the last two decades, however, there has been a major paradigm shift in our mechanistic thinking in vascular biology—one that places a primary emphasis on the active involvement of the cells that comprise the arterial wall, in particular endothelium and smooth muscle, in vascular disease processes such as atherosclerosis.1,4 In this context, an alternative hypothesis linking hemodynamic forces and atherosclerosis has emerged, one which already has generated a number of novel mechanistic insights. The central premise of this new working hypothesis is that the endothelial lining, as the cellular component in direct contact with flowing blood, is the primary sensor of wall shear stresses and functions as a transducer of these biomechanical stimuli into biological responses within the vessel wall.5-7
Inferential evidence that vascular endothelial cells in vivo are responsive to local flow conditions comes from en face morphological analyses of cell shape in different regions of the arterial intima. In areas of uniform laminar flow, endothelial cells exhibit an ellipsoidal cell (and nuclear) shape and alignment in the direction of flow, whereas in regions of disturbed flow, this orderly pattern is disrupted.8,9 In addition, arterial wall remodeling (eg, after experimental surgical coarctation or shunting procedures) appears to depend at least in part on a functionally intact endothelium.10 Direct evidence that hemodynamic forces can influence endothelial structure and function has come from studies in which cultured endothelial cells have been subjected to defined fluid mechanical forces under well controlled conditions in vitro. In these simplified model systems, unidirectional steady laminar shear stresses of physiological amplitude induce time- and force-dependent endothelial cell shape change and alignment that is gradually reversible upon the cessation of flow.11-13 These shear-induced changes in cell shape are accompanied by reorganization of actin filaments and other cytoskeletal components, thus mimicking the morphology of aortic endothelium in vivo. Additionally, work by numerous laboratories has demonstrated a variety of changes in the metabolic and synthetic activities of endothelial cells in response to defined flow stimulation, including the production of prostacyclin, nitric oxide, cytokines, growth factors, extracellular matrix components, and vasoactive mediators.5-7,14 Some of the more acute changes involve regulation at the level of rate-limiting enzymes and/or substrate availability (eg, arachidonic acid release by calcium-sensitive phospholipases, NO production by nitric oxide synthase). However, in many cases where the responses are delayed and/or sustained, modulation of endothelial gene expression appears to be occurring.
How is the frictional force of blood flow along its luminal surface
sensed by the endothelial cell and transduced into molecular biological
events such as gene regulation? At present, the fundamental question of
the identity, location, and mechanism(s) of action of endothelial
flow-sensitive mechanotransducers remains a challenging one. Several
distinct molecules, eg, cell surface ion channels, various
receptor-associated G proteins, and members of the mitogen-activated
and stress-activated protein kinase cascades, as well as certain
transcription factors such as nuclear factor-
B, c-Fos, and Egr-1,
are rapidly activated in response to fluid shear stresses applied to
the endothelial cell surface.5,6,14-17
In addition,
cellular organelles, such as the cytoskeleton (intermediate filaments,
microtubules, actin-myosin stress fibers), plasma membrane caveoli,
lateral cell-cell junctional proteins, basal focal adhesion complexes,
and even the lipid bilayer of the plasma membrane, also appear to be
participating in shear-induced endothelial responses. Finally, various
second messengers, including ionized cytosolic calcium, intracellular
lipid products of the polyphosphoinosotide pathway, and nitric oxide,
are generated in the context of flow-stimulation of endothelium. As
discussed by Davies,5
the challenge is to understand the
interaction of these spatially and temporally disparate components in
the dynamic interplay of the endothelial cell's response to
biomechanical stimulation—sorting out where transmission becomes
transduction, as well as cause-effect relationships. For example, one
might envision the endothelial cell as analogous to a circus tent and
the wind blowing across the roof of the tent as the frictional force
(wall shear stress) generated by blood flow. As the wind blows, the
ropes tethering the tent roof (analogous to cytoskeletal stress fibers)
transmit fluctuating forces to the stakes (analogous to
integrin-containing focal adhesion complexes) anchored in the ground
around the base of the tent. The resulting movement of the stakes in
the earth might be likened to phosphorylation/dephosphorylation and
other biochemical events occurring in the focal adhesion complexes
distributed along the basal aspect of the cell, which then are
functioning as biomechanical-biochemical transducers at a distance from
the point of application of the force. Similar actions at a distance
might be envisioned between apical membrane and lateral cell-cell
junctions, and/or the nuclear envelope.5
Indeed, as
Ingber18
has suggested, the entire cytoskeleton may
participate in what he called a tensegrity-based
stimulus-response coupling, which dynamically integrates externally
applied stresses, extracellular matrix attachments, and internal
strains into adaptive biological responses. Given that the endothelial
cell cytoskeleton and focal adhesion complexes can actively be
remodeled in response to applied forces5
and that there
are regional differences in the magnitude of shear stresses across the
surface of a given endothelial cell (reflecting details of its surface
topography),19,20
the potential for significant
heterogeneity in responsiveness among cells within a uniform flow field
also exists. Interestingly, although this is a relatively complex model
for biomechanical transduction, considerable observational data support
it. In contrast, at present there is no clear-cut example of an
intrinsic membrane protein whose deformation by shear stresses directly
results in transmembrane signal transduction, ie, a candidate shear
receptor.
Regardless of the proximal sensing mechanism(s), how does a given gene
within the endothelial nucleus become transcriptionally activated in
response to shear stress? Using the human platelet-derived growth
factor-B chain (PDGF-B) as a model of a shear-responsive gene in
endothelial cells, Resnick and coworkers21
defined the
first example of a "shear stress response element (SSRE)." This
SSRE, consisting of a simple 6-bp (GAGACC) motif in the proximal
promoter region, was shown to function as a necessary and sufficient
cis-acting transcriptional regulatory element, mediating the
up-regulation of PDGF-B expression in response to physiological levels
of applied fluid shear stresses. Subsequent studies22
established that components of the NF-B complex could interact
functionally with this SSRE to mediate transcriptional activation of
the PDGF-B gene. Previous work had established that NF-B was rapidly
activated, as evidenced by its translocation to the nuclear
compartment, in response to physiological levels of laminar shear
stress (LSS). Taken together, these studies thus established the first
model for coordinate trans-activating and
cis-activating transcriptional regulation in response to
biomechanical stimulation in the endothelial cell. Subsequent studies
have established the existence of various other SSREs in several
pathophysiologically relevant endothelial genes (eg, monocyte
chemoattractant protein-1, tissue factor, endothelin-1, VCAM-1)
which appear to be at least partly responsive to biomechanical
stimulation. Interestingly, in the case of VCAM-1, the relevant SSRE
appears to mediate the down-regulation of transcription in response to
LSS stimulation.23
Indeed, recent
studies24
have shown that LSS stimulation can blunt the
up-regulation of VCAM-1 in endothelial cells activated by various
proinflammatory cytokines. These observations may have special
relevance to the basic question of atherosclerotic lesion localization
in vivo, as will be considered in greater detail below. Some
further points should be noted with regard to SSREs and shear stress
gene regulation in endothelial cells. Many genes contain one or more
copies of the various SSREs identified to date, yet not all of these
genes are necessarily shear stress-responsive. Indeed, SSREs
appear to function in a context-specific manner, in that a given
element can mediate transcriptional responses to shear stress in the
context of one promoter and yet not do so in the context of
another promoter.14
This has been illustrated in studies
of LSS induction of leukocyte-endothelial adhesion molecules, including
E-selectin, ICAM-1, and VCAM-1, each of which contains functional
NF-B elements but responds differently (ICAM-1, up-regulation;
VCAM-1, no change or down-regulation; E-selectin, no change) in the
same cell exposed to the same biomechanical
stimulus.25,26
It should also be noted that certain
transcription factor genes, eg, Egr-1 and c-fos, are themselves
shear-responsive,27
thus raising the possibility that they
may function in the induction of multiple other genes in the
orchestration of an endothelial cell's response to biomechanical
stimulation.
Do differences in the temporal and/or spatial characteristics of flow
elicit different patterns of endothelial gene expression? Studies by
several laboratories using different in vitro model systems
clearly indicate that endothelial cells can sense differences in the
temporal and/or spatial characteristics of flow and translate these
biomechanical stimuli into different biological responses. For example,
steady laminar flow appears to enhance endothelial cell survival by
suppressing apoptosis,26,28
whereas turbulent flow can
trigger endothelial cell division.29
Differences in the
temporal properties of laminar flow stimulation, generated by
instantaneous (impulse) versus gradual (ramp) application of
the same final level of shear stress, can elicit very different
responses in endothelial gene expression.30,31
Similarly,
oscillatory versus steady laminar flows elicit marked
differences in the pattern of adhesion molecule expression in cultured
endothelium.32
To focus more specifically on the effects
of spatial gradients in shear stress on endothelial biology, DePaola et
al33
developed an in vitro model system that
generates large gradients in shear stress over the relatively small
dimensions of a cultured endothelial monolayer, thus mimicking the
spatial pattern of flow separation (with reversal), reattachment, and
flow recovery associated with arterial bifurcations in vivo.
Using this in vitro spatial disturbed flow model, dramatic
differences in endothelial cell shape, migration, and proliferation
have been demonstrated in association with disturbed flow as compared
with uniform laminar flow.34
In addition, significant
differences in endothelial expression of Connexin43 at the level of
mRNA and protein, and concomitant changes in cell-cell communication
via gap junctions, also have been correlated with the presence of shear
stress gradients in this model.35
Recent studies suggest
that these in vitro observations may indeed have a
counterpart in vivo.36
Most recently, Nagel and
coworkers37
have demonstrated that endothelial cell
monolayers exhibit significant spatial heterogeneity in the nuclear
localization of certain critical transcription factors, including
NF-B, Egr-1, c-jun, and c-fos, and that these differences correlate
with the local shear stress gradient. Taken together, these studies
thus strongly suggest that spatial gradients of wall shear stress, in
contrast to absolute shear stress magnitudes, can be important
determinants of endothelial responses at the level of gene regulation.
Further studies are needed to elucidate the potential interplay of both
temporal and spatial fluctuations in the biomechanical regulation of
endothelial gene expression and, ultimately, to correlate these stimuli
with the endothelial phenotypes actually observed in different
in vivo biomechanical environments.
How might flow-induced changes in endothelial phenotype contribute to atherogenesis in vivo? To more systematically address the question of modulation of endothelial phenotype by biomechanical stimuli, our laboratory has turned to high- throughput molecular biological strategies. Specifically, we have used a reverse transcription-polymerase chain reaction-based, high-throughput differential display of transcripts to compare the patterns of genes that are up-regulated or down-regulated in human endothelial cells in response to physiological levels of steady laminar shear stress, a comparable level of turbulent (nonlaminar) shear stress, and a soluble cytokine stimulus (interleukin-1ß) at a maximally effective concentration.14 This approach has revealed distinctive patterns of endothelial gene expression not previously appreciated, including a set of genes that appear to be up-regulated in a sustained fashion by steady laminar shear stress but not by turbulent shear stress. Certain of these differentially regulated transcripts encode known endothelial genes of relevance to atherogenesis, such as eNOS or NOSIII (the endothelial isoform of nitric oxide synthase), COX-2 (the inducible isoform of cyclooxygenase), and Mn-SOD (manganese-dependent superoxide dismutase). These endothelial genes encode enzymes that exert potent anti-thrombotic, anti-adhesive, anti-proliferative, anti-inflammatory, and anti-oxidant effects, both within the endothelial lining and in interacting cells such as platelets, leukocytes, and vascular smooth muscle. The biological consequences of these steady laminar shear up-regulated endothelial genes thus would be predicted to be vasoprotective or anti-atherogenic.14,38
Given that uniform laminar shear stresses are characteristically associated with atherosclerotic lesion-protected arterial geometries in vivo, these observations have led us to hypothesize that this type of biomechanical stimulus up-regulates the expression of a subset of "atheroprotective genes" in endothelial cells, which then act locally in the lesion-protected areas to offset the effects of systemic risk factors such as hypercholesterolemia, hyperhomocysteinemia, hyperglycemia (diabetes), and hypertension.4,14,27 The coordinated and selective up-regulation of atheroprotective genes by uniform laminar shear stress is a possible mechanistic link between the local hemodynamic milieu, endothelial gene expression, and early events in atherogenesis, thus providing a potential explanation for the nonrandom localization of early atherosclerotic lesions. This working hypothesis does not exclude the potential direct action of complex disturbed laminar flows, such as occur in lesion-prone arterial geometries, as stimuli for the expression of pro-atherogenic genes (eg, adhesion molecules, growth factors, cytokines).27
Critical testing of the "atheroprotective gene" and related hypotheses will depend on refinement of both in vitro and in vivo fluid mechanical models, and their applicability to vascular disease pathobiology at the molecular genetic level. The development of reliable methods for the linear amplification of transcripts from small numbers of cells and their analysis by DNA chip microarrays or analogous genome scale technologies hold much promise in this regard. Application of these comprehensive and relatively unbiased methods of molecular analysis to endothelial cells subjected to experimentally defined flow conditions will add significantly to our understanding of the dynamic range of biomechanically induced phenotypic modulation. Ultimately, the extension of this approach to dysfunctional vascular endothelium in the natural disease context should provide new insights into the link between hemodynamic forces and atherogenesis.
Acknowledgements
I wish to acknowledge the many past and present members of the Vascular Research Division at the Brigham and Women's Hospital, notably N. Resnick, J. Topper, T. Collins, L. Khachigian, T. Nagel, G. Garcia-Cardena, K. Anderson, and B. Blackman, for their conceptual input and experimental collaboration in the original research studies summarized here and the longstanding collaboration of Prof. C. F. Dewey and colleagues in the Fluid Mechanics Laboratory at the Massachusetts Institute of Technology.
Footnotes
Address reprint requests to Dr. Michael A. Gimbrone, Jr., Department of Pathology, Brigham and Women's Hospital, 221 Longwood Avenue, LMRC 4th Floor, Boston, Massachusetts 02115-6195. E-mail: gimbrone{at}bustoff.bwh.harvard.edu
Supported in part by the National Heart Lung and Blood Institute and Millennium Pharmaceutics, Inc., Cambridge, MA.
Accepted for publication May 18, 1999.
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 G. Lembo, N. De Luca, C. Battagli, G. Iovino, A. Aretini, M. Musicco, G. Frati, F. Pompeo, C. Vecchione., and B. Trimarco A Common Variant of Endothelial Nitric Oxide Synthase (Glu298Asp) Is an Independent Risk Factor for Carotid Atherosclerosis Stroke, March 1, 2001; 32(3): 735 - 740. [Abstract] [Full Text] [PDF]
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J. W. Knowles and N. Maeda Genetic Modifiers of Atherosclerosis in Mice Arterioscler. Thromb. Vasc. Biol., November 1, 2000; 20(11): 2336 - 2345. [Abstract] [Full Text] [PDF]
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L. Hajra, A. I. Evans, M. Chen, S. J. Hyduk, T. Collins, and M. I. Cybulsky The NF-kappa B signal transduction pathway in aortic endothelial cells is primed for activation in regions predisposed to atherosclerotic lesion formation PNAS, August 1, 2000; 97(16): 9052 - 9057. [Abstract] [Full Text] [PDF]
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G. E. Caughey, L. G. Cleland, J. R. Gamble, and M. J. James Up-regulation of Endothelial Cyclooxygenase-2 and Prostanoid Synthesis by Platelets. ROLE OF THROMBOXANE A2 J. Biol. Chem., October 5, 2001; 276(41): 37839 - 37845. [Abstract] [Full Text] [PDF]
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