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(American Journal of Pathology. 2000;156:797-805.)
© 2000 American Society for Investigative Pathology

Short Communications

Elevated Aß42 in Skeletal Muscle of Alzheimer Disease Patients Suggests Peripheral Alterations of AßPP Metabolism

Yu-Min Kuo*, Tyler A. Kokjohn{dagger}, M. Desiree Watson{ddagger}, Amina S. Woods§, Robert J. Cotter§, Lucia I. Sue, Walter M. Kalback*, Mark R. Emmerling{ddagger}, Thomas G. Beach and Alex E. Roher*

From the Haldeman Laboratory for Alzheimer Disease Research*
and the Civin Laboratory of Neuropathology,
Sun Health Research Institute, Sun City, Arizona; the Department of Microbiology,{dagger}
Midwestern University, Glendale, Arizona; the Department of Pharmacology and Molecular Sciences,§
Johns Hopkins University School of Medicine, Baltimore, Maryland; and the Department of Neuroscience and Therapeutics,{ddagger}
Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, Michigan


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 References
 
The levels of amyloid-ß40 (Aß40) and Aß42 peptides were quantified in temporalis muscles and brain of neuropathologically diagnosed Alzheimer disease (AD) and of nondemented individuals. This was achieved by using a novel analytical approach consisting of a combination of fast-performance liquid chromatographic (FPLC) size exclusion chromatography developed under denaturing conditions and europium immunoassay on the 4.0- to 4.5-kd fractions. In the temporalis muscles of the AD and nondemented control groups, the average values for Aß42 were 15.7 ng/g and 10.2 ng/g (P = 0.010), and for Aß40 they were 37.8 ng/g and 29.8 ng/g (P = 0.067), respectively. Multiple regression analyses of the AD and control combined populations indicated that 1) muscle Aß40 and muscle Aß42 levels were correlated with each other (P < 0.001), 2) muscle Aß40 levels were positively correlated with age (P = 0.036), and 3) muscle Aß42 levels were positively correlated with Braak stage (P = 0.042). Other forms of the Aß peptide were discovered by mass spectrometry, revealing the presence of Aß starting at residues 1, 6, 7, 9, 10, and 11 and ending at residues 40, 42, 44, 45, and 46. It is possible that in AD the skeletal muscle may contribute to the elevated plasma pool of Aß and thus indirectly to the amyloid deposits of the brain parenchyma and cerebral blood vessels. The increased levels of Aß in the temporalis muscles of AD patients suggest that alterations in AßPP and Aß metabolism may be manifested in peripheral tissues.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 References
 
The profuse accumulation of amyloid-ß (Aß) peptide in the extracellular space of the cerebral cortex and walls of cerebral and leptomeningeal blood vessels represents one of the major histopathological lesions of Alzheimer disease (AD). The Aß peptide is derived from the normal proteolytic processing of a larger Aß precursor protein (AßPP), which in the brain is expressed in neurons, glial cells, and vascular myocytes.1 However, peripheral tissues such as heart, liver, pancreas, thyroid, lymph nodes, spleen, skeletal muscle, salivary and adrenal glands, skin, intestine, leukocytes, and platelets also express AßPP.2-6 In addition, the presence of AßPP has also been confirmed in the skeletal muscle neuromuscular junctions by immunofluorescence confocal microscopy and immunoelectron microscopy.6 The exact biochemical roles of AßPP and Aß remain unknown, although some important functions for these molecules have been suggested.1 It is interesting that, during the course of acute phase reactions such as traumatic brain injury, the AßPP is up-regulated and associated with transient elevation of Aß level.7,8 Although it has been largely accepted that the origin of the Aß deposited in the brain is the brain tissue itself, recent investigations have demonstrated that circulating Aß is capable of crossing the blood brain-barrier (BBB), implying a potentially hematogenous source for the brain’s parenchymal and vascular Aß peptides.9 One possible source for the circulating Aß is the skeletal muscle. Transgenic mice overexpressing the last 99 amino acids of human AßPP in peripheral tissues produce high circulating levels of human Aß in the plasma.10 Moreover, individuals suffering from inclusion body myositis, the most common muscle disease observed in the elderly, exhibit prominent intracytoplasmic vacuoles containing Aß immunoreactive filaments.11 A systematic study comparing the levels of Aß in brain and skeletal muscle in AD and control individuals has not been performed. In this investigation we quantified for the first time the Aß40 and Aß42 in the temporalis muscles of demented individuals neuropathologically confirmed to have AD. These values were compared with those obtained from a cohort of elderly nondemented control individuals.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 References
 
Materials

The equipment and materials used in the separation and immunoassay of Aß were from the following sources. Fast-performance liquid chromatography (FPLC) equipped with fraction collector and Superose 12 size exclusion column were from Pharmacia Biotech (Uppsala, Sweden). Tris(hydroxymethyl)aminomethane (Tris), acetonitrile, NaOH, Tween 20, NaCl, and Na2CO3 were from Sigma Chemical Co. (St Louis, MO). Paraformaldehyde was from Fisher Scientific (St. Louis, MO). Hydrochloric acid and formic acid (98%) were from Fluka Chemie AG (Buchs, Switzerland). The formic acid was in all instances further purified in our laboratory by glass distillation. Oligonucleotides, restriction enzyme HhaI, and all of the reagents used for ApoE genotyping were obtained from Genosys (The Woodlands, TX) and GIBCO/BRL (Gaithersburg, MD). The monoclonal antibody 4G8, raised against residues 17–24 of the Aß peptide (Senetek, Maryland Heights, MO), was labeled with europium (Eu) by the procedures given by the manufacturer (Wallac Inc. Gaithersburg, MD). The Eu, enhancement solution, and fluorimeter were from Wallac Inc. The polyclonal antibodies R163 and R165, specifically raised against Aß40 and Aß42,12 respectively, were obtained from P. Mehta (New York Institute for Basic Research, New York, NY). These antibodies do not exhibit cross-recognition of the two forms of Aß molecules at concentrations >100 ng/ml. Microtiter plates were purchased from Nalge Nunc International (Denmark). The antibody 22C11, raised against the N terminus of AßPP, was obtained from Roche Molecular Biochemicals (Indianapolis, IN).

Human Tissues

The brains from 43 elderly individuals, 23 AD (mean age, 85.6; range 79–96 years) and 20 nondemented (mean age, 77.7; range 63–91 years), were removed and coronally sectioned into 1-cm slices. The left hemispheres were fixed in 4% buffered paraformaldehyde and used for histopathological analyses. The right hemispheres were immediately frozen at -85°C. Postmortem delay (time from death to freezing) averaged 2.5 hours (range, 1–5 hours).

All AD (cases 1–23) and nondemented control (cases 101–120) brains were obtained from the brain bank at the Sun Health Research Institute (Table 1) . All brains were bequeathed on a strictly voluntary basis, with the expectation that the tissue would be used for research in AD and other neurodegenerative diseases. AD cases were diagnosed neuropathologically according to the criteria established by the Consortium to Establish a Registry for Alzheimer Disease (CERAD).13 The Braak stage classification was also considered.14 Dementia with Lewy bodies (DLB) cases and progressive supranuclear palsy (PSP) cases were diagnosed according to published consensus criteria.15-17 Control cases, including neuropathologic conditions other than AD as well as nondemented cases free of neuropathologic abnormalities, were also rated by Braak stage and CERAD neuritic plaque density.


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  		Table 1. Neuropathological Diagnosis

 
Sections (40 µm each) were stained by hematoxylin-eosin (H&E), thioflavine-S, Gallyas, and Campbell-Switzer silver techniques. All individuals were ApoE genotyped by the technique of Hixson and Vernier,18 using blood obtained by cardiac puncture in the immediate postmortem. Skeletal muscles (right and left temporalis) were carefully removed at autopsy and kept frozen at -85°C until the moment of use. The temporalis muscles were studied because they are easily accessible during the removal of the brain. The temporalis muscles have a mixed population of fiber types19 and are similar to most of the skeletal muscles in the human body.20

Isolation and Quantification of Aß Peptides

Temporalis muscle (200 mg), free of adipose and connective tissue, was finely minced with a razor blade. The tissue was thoroughly disrupted in 12 ml of 90% formic acid by using a Dounce glass homogenizer. The specimens were loaded into 12-ml polyallomer tubes and centrifuged for 30 minutes at 250,000 x g in a Sorvall TH-641 rotor at 5°C. A sample of 500 µl was carefully taken from the middle of the tube and loaded onto a Superose 12 size exclusion column. The column was equilibrated, and the chromatography was developed with 80% glass distilled formic acid. Fractions corresponding to the retention time of 4.5 kd (defined by the synthetic Aß reverse sequence 40–1) were collected, pooled, and mixed with 30 µl of 10% betaine, and the acid was immediately eliminated by vacuum centrifugation. The dried specimens were dissolved in 50 µl of 80% formic acid, which were then diluted with 250 µl of 0.5 mol/L Tris/HCl, pH 8.0, 1.37 mol/L NaCl, 27 mmol/L KCl, and 0.5% Tween 20. The volume was brought to 1 ml with distilled water, and the pH was adjusted to 7.4 with 10 N NaOH with a pH meter equipped with a microelectrode.21 The final volume was brought to 2.5 ml by the addition of distilled water. To prepare the microtiter plate, 50 µl of the capture antibody, either R163 or R165, at a concentration of 10 µg/ml in 10 mmol/L Na2CO3, pH 9.6, was added to the wells of microtiter plates and left at room temperature for 2 h. A blocking solution of 1% bovine serum albumin in TTBS (0.05% Tween 20 in 50 mmol/L Tris/HCl, 137 mmol/L NaCl, 2.7 mmol/L KCl, pH 7.4) was added to each well and incubated at room temperature for 1 hour. Either 100 µl of the specimens under investigation or of the Aß40 and Aß42 standards were applied in triplicate to the wells and allowed to stand at room temperature for 2 hours on a rocking platform. The unbound materials were removed by washing the plate 3 times with TTBS. Fifty µl of Eu-labeled 4G8 antibody (4 µg/ml) were added to the wells and incubated for 1 hour, followed by four washes with TTBS and three washes with distilled water. Finally, 50 µl of Enhancement solution were added to each well, and the plates were read in a fluorimeter by using excitation and emission wavelengths of 320 and 615 nm, respectively. A standard curve was plotted for each plate, and sample Aß concentrations were calculated with reference to these standard curves. The Aß40 and Aß42 peptides used to construct the standard curves were dissolved in dimethylsulfoxide (DMSO) at a concentration of 1 mg/ml and subsequently diluted with TTBS to the required concentrations (from 25 to1000 pg/ml).

Superior frontal gyrus (250 mg) was thoroughly homogenized in the presence of 12 ml of 98% formic acid. The brain specimens were centrifuged as described for the muscle tissue. This permitted the separation of an insoluble pellet and of a small amount of lipid material that floated at the top of the supernatant. An aliquot of 500 µl from each specimen was chromatographically separated, and the 4.5-kd fraction was isolated, dried, suspended in formic acid, pH adjusted to neutrality, and europium immunoassayed as previously described for the temporalis muscle.

Mass Spectrometry

Mass spectra were acquired on a Kratos Kompact MALDI IV time-of-flight mass spectrometer (Manchester, UK) in a positive linear mode with a 337-nm N2 laser and a 20-kV extraction potential. An aliquot of the relevant FPLC fractions was lyophilized and suspended in 10 µl of glass-distilled formic acid, of which 0.3 µl was deposited on a sample probe, followed by the addition of 0.3 µl of matrix consisting of a saturated solution of {alpha}-cyano-4-hydroxy-cinnamic acid in 50% ethanol. Reported spectra were the average of 50 laser shots. Each spectrum was calibrated using external standards.

AßPP Western Blot Quantification

Four hundred µg of muscle or 300 µg of brain tissue were homogenized in 1.6 ml of buffer: 20 mmol/L Tris-HCl, pH 8.4, 0.2% Triton X-100, and a protease inhibitor cocktail. Homogenates were centrifuged at 12,000 x g for 15 minutes at 4°C, and the supernatants were submitted to Western blotting. The homogenates were diluted with sample buffer (Novex, San Diego, CA) to equal protein concentrations and loaded onto 16% tricine or 10% Tris-Glycine precast gels (Novex). The electrophoretically separated proteins were transferred onto nitrocellulose membranes and blocked with 5% nonfat milk in Tris-buffered saline (TBS; 20 mmol/L Tris-HCl, pH 7.4. 0.5 mol/L NaCl). The primary antibody 22C11 (10 µg/ml) made to the N terminus of AßPP was incubated with blots overnight in 1% milk-TBS and blots were washed with TTBS. Secondary anti-mouse antibodies (Amersham; 1:750) for 22C11, diluted in milk-TBS, were added to the blots and incubated for 1 hour. The washed blots were developed using the Pierce (Rockford, IL) chemiluminescent detection system and exposed to film, and the band densities were analyzed with a Kodak Digital Imaging System.

Aß Western Blot Detection

FPLC-separated, 4.5-kd fractions from six runs of temporalis muscle samples were pooled and dried by vacuum centrifugation. The samples were dissolved in tricine-sodium dodecyl sulfate (SDS) sample buffer (Novex) and separated in a 10% to 20% tricine gel (Novex). The peptides were transferred onto a polyvinylidene difluoride (PVDF) membrane (BioRad) and reacted with a mixture of Aß antibodies 4G8 and 6E10 as previously published.21 The membranes were developed as described for AßPP blots.

Immunocytochemistry

Temporalis muscles from four cases with high levels of Aß on the EuIA were stained immunocytochemically for Aß with an antibody that recognizes residues 1–16 (monoclonal antibody 10D5, Athena Neurosciences, South San Francisco, CA). Sections were also stained with the antibodies R163 and R165, which recognize Aß peptides ending in residues 40 and 42, respectively. Paraffin-embedded muscle tissues were cut at 8 µm, deparaffinized, and treated with 90% formic acid for 5 minutes. Sections were incubated in primary antibodies at 1:1000 overnight at 4°C and followed by a biotinylated secondary antibody and an avidin-biotin-peroxidase complex (ABC, Vector Laboratories, Burlingame, CA) using 3,3'-diaminobenzidine as the substrate, as previously described.22 Control sections were treated identically except for the omission or preabsorption of the primary antibody with 100 µg/ml synthetic Aß 1–40. A section of frontal cortex from an AD case was used as a positive control.

Statistical Analyses

The two-tailed Student’s t-test was applied when variable means were compared between AD and control subjects. Multiple stepwise (backward) regression was used to analyze the relationships among the specified dependent variables and independent variables in the combined control and AD populations (n = 43). At each step the variable that contributed least to the result was removed, as determined by t-tests of the regression coefficients. The regression was stopped at the point in which all of the probabilities of the variables were smaller than 0.05. The apoE genotypes were assigned the following numerical scale: 1 = no apoE {epsilon}4; 2 = apoE {epsilon}2/{epsilon}4 or apoE {epsilon}3/{epsilon}4; 3 = apoE {epsilon}4/{epsilon}4. Neuritic plaque densities were converted to numerical scores as follows: none = 0; sparse = 1; sparse/moderate = 2; moderate = 3; moderate/frequent = 4; frequent = 5.


   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 References
 
Neuropathological examination established that, in this study, 21 individuals met the CERAD criteria for AD (Table 1) . Two cases (19 and 20) were, as far as we could investigate, nondemented, but met the neuropathological criteria for AD and therefore were included in the CERAD-positive group. These two cases probably belong to the cohort of high-pathology individuals, who, despite having considerable numbers of neuritic plaques and neurofibrillary tangles, do not exhibit the symptoms of dementia.23 Within the CERAD-positive group, 11 subjects presented supplementary pathological changes such as multiple infarcts, Lewy bodies, hippocampal sclerosis, or signs of progressive supranuclear palsy. In addition, three more patients had the neuropathological and clinical symptoms of Parkinson’s disease. In the control nondemented group, none of the 20 individuals exhibited symptoms of dementia or met the CERAD standards for AD, with the exception of four cases that had borderline CERAD values and therefore could be considered as possible AD. In four cases no pathological changes were observed. Parkinson’s disease was present in seven individuals. In addition, three cases had hippocampal sclerosis, three cases had progressive supranuclear palsy, and one case each exhibited Lewy body disease and argyrophilic grain disease (Table 1) . The allele frequencies for Apo {epsilon}2, {epsilon}3, and {epsilon}4 in the AD group were 4%, 74%, and 22%, respectively, whereas in the nondemented group they represented 5%, 87%, and 8%, respectively. The AD group had a significantly higher {epsilon}4 allele frequency than the nondemented control group ({chi}2 = 7.69, P = 0.006).

The chromatographic separation of the 4.5-kd fractions containing the Aß peptides, under denaturing conditions, allows for the partition of Aß peptides from other molecules with higher and lower Mr that interact with Aß (Figure 1) . Western blotting of the 4.5-kd chromatographic fractions from AD and control temporalis muscles demonstrated the presence of three Aß-immunopositive bands that may represent monomers, dimers, trimers, or tetramers of Aß (Figure 2) . The quantification of the Aß peptides by EuIA in the brains of AD and control individuals clearly demonstrated that, on average, the levels of these peptides were much higher in AD (Table 2) . The amounts of Aß40 in the AD and control brains were 608 ng/g and 209 ng/g, respectively (P = 0.022). The levels of Aß42 in the AD and control brains were 6096 ng/g in the former and 784 ng/g in the latter (P < 0.001). The differences in Aß40 and Aß42 in the AD brain approximate those observed in chemically purified AD plaque cores, in which about 90% of the Aß ends at residue 42.24



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  		Figure 1. Chromatographic profile of the temporalis muscle proteins. The solid trace depicts the chromatographic contour of the muscle proteins produced by a size exclusion Superose 12 column (1 x 30 cm) developed with 80% glass-distilled formic acid. The separation was carried out using a FPLC apparatus (Pharmacia Biotechnology) at a flow rate of 15 ml per hour at room temperature and monitored at 280 nm. Fractions were automatically collected every 2 minutes. To define the retention time of the Aß-containing fractions in the muscle preparation, an unused column was calibrated with synthetic Aß reverse amino acid sequence 40–1 (molecular mass, 4331) which is indicated by the hyphened trace. After this calibration, extreme care was taken to decontaminate the injection system by multiple formic acid injections until, in a mock run, there were no traces of Aß in the eluant as detected by EuIA. The Aß eluted between 56 and 62 minutes was collected, pooled, and mixed with betaine (see Materials and Methods). The addition of this zwitterion prevents undesirable adsorption of Aß to the glass during the drying process. The arrows indicate the elution times of calibration markers separated under the same denaturing conditions (in order from left to right): bovine serum albumin, ovalbumin, chymotrypsinogen, cytochrome C, Aß dimer, Aß monomer, and bacitracin.

 


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  		Figure 2. Western blot of the chromatographically separated 4.5-kd fraction from AD and control temporalis muscles. In each case, six chromatographic separations were pooled, totally dried by vacuum centrifugation, dissolved in tricine-SDS sample buffer (Novex), and separated in a 10% to 20% tricine gel. The peptides were transferred onto a PVDF membrane (BioRad) and reacted with a mixture of Aß antibodies 4G8 and 6E10. The membranes were developed as previously published.21 The numbers on the right margin indicate the position of molecular weight markers.

 

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  		Table 2. Muscle and Brain Aß Concentrations in AD and Control Subjects

 
The presence of formic acid may alter Aß structure in such a way that, in the immunoassay, the binding of antibodies is enhanced. To investigate this possibility, the Aß1–40 and Aß1–42 standard curves for the EuIA were constructed using either DMSO or formic acid (80%) as initial dissolving agents. Before dilution with TTBS, the formic acid was neutralized using the same procedure as used for the tissue samples. No statistically significant differences in the amount of Aß detected were found between the two procedures: Aß40, r = 0.960; Aß42, r = 0.968.

In the temporalis muscles of the AD and nondemented control groups, the average values for Aß42 were 15.7 ng/g and 10.2 ng/g (P = 0.010), and for Aß40 were 37.8 ng/g and 29.8 ng/g (P = 0.067), respectively. The amounts of Aß were distributed in a wide range: Aß40, AD 18.6–80.4 ng/g, versus control, 15.1–52.3 ng/g; Aß42, AD 8.2–30.1 ng/g, versus control, 0.8–24.4 ng/g (Table 2) .

Several multiple regressions were used to analyze the relationships among the variables shown in Tables 1 and 2 . When apoE was chosen as a dependent variable, the resulting regression demonstrated that neuritic plaque density score (P = 0.024) and brain Aß40 (P < 0.001) were significantly correlated at the 95% confidence level (r = 0.608). Likewise, there was a strong positive interaction (r = 0.843) between the Braak stage and neuritic plaque density score (P < 0.001), brain Aß40 (P = 0.006), and a somewhat less pronounced effect correlated with the muscle Aß42 (P = 0.015). When muscle Aß42 was chosen as the dependent variable, muscle Aß40 and Braak stage showed significant positive correlations (P < 0.001 and P = 0.042, respectively). It is interesting that the regression analysis revealed that there were positive correlations between age and neuritic plaque density score (P < 0.001) and age and muscle Aß40 (P = 0.036). There were no apparent associations between gender and the muscle or brain Aß levels in the AD and control populations.

Mass spectrometry of the isolated Aß from the temporalis muscles produced a serendipitous finding: the presence of Aß peptides ending at residues 44, 45, and 46 (Aß notation) in both the nondemented control cases and AD individuals (Table 3) . It is unlikely, given the specificity of our antibodies, that these Aß forms were detected in our immunoassays. Quantification of these longer Aß peptides awaits further investigation because the relative intensities of the molecular ion signals in the mass spectrum of a single sample do not necessarily reflect their relative abundance. In addition, mass spectrometry revealed the presence of Aß peptides beginning at positions 6, 7, 9, 10, or 11.


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  		Table 3. Mass Spectrometry of Aß Peptides from Skeletal Muscle

 
The determination of muscle and brain relative levels of AßPP in both populations demonstrated no significant differences between the two cohorts in terms of relative units of density (Figure 3) . Last, temporalis muscle sections from AD and nondemented cases, stained for Aß using three different specific antibodies (10D5, R163, and R165), revealed no differences between the two groups.



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  		Figure 3. The relative levels of AßPP in the temporalis muscle and brain. A: A representative Western blot of AßPP stained by 22C11 from muscle obtained from control (C) and Alzheimer (A) tissues is shown. B and C: Histograms showing the relative levels of the 100- and 110-kd AßPP in the temporalis muscle (B) and the brain (C). The Western blots were densitometrically scanned, and the relative band intensities of the AD and control groups were averaged. There was no statistically significant difference between the two cohorts.

 

   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
References
 
A milestone in understanding the pathophysiology of AD was the discovery of Aß40 and Aß42 peptides in the brain and biological fluids of normal individuals.25-27 For AD, these observations unseated the dogma that Aß accumulation in this dementia was the result of an anomalous AßPP proteolytic degradation. This insight also ushered in alternative explanations for the pathogenic mechanisms of amyloidosis, such as Aß overproduction, Aß conformational misfolding, and defective Aß clearance. In addition, it reopened the door for an earlier view postulating that a hematogenous source of Aß could serve as a substrate for the vascular and parenchymal amyloid deposits seen in the AD brain.28,29

The current results indicate that in AD there is a significant elevation of Aß in the temporalis muscles relative to that in nondemented individuals. In the former, the mean value for total Aß was 53.5 ng/g, whereas in the latter it was only 40.0 ng/g. This observation leads to some interesting prospects, the most striking of which is that the peripheral tissue metabolism of AßPP may be disturbed as reflected in the elevated muscle Aß levels present in AD patients. This is an unprecedented finding because it provides an indication that AD may be a systemic disease rather than exclusively a disease of the central nervous system. The increased levels of plasma Aß30 might result from skeletal muscle abnormalities, much as serum Aß levels are elevated in transgenic mice that overexpressed the AßPP C-99 transgene only in peripheral tissues.10

Our study reveals for the first time that human skeletal muscle tissue possesses measurable amounts of Aß40 and Aß42. Because skeletal muscle represents about one quarter of body weight in humans, the pool of muscle Aß could, over time, contribute to a substantial build up of Aß in plasma and ultimately the brain. Alternatively, Aß produced in the brain could be released into circulation and taken up by skeletal muscle. A series of recent observations supports the contention that peripherally circulating Aß is a potential contributor to the cerebral amyloidosis of AD.31,32 After intravenous injections of Aß in aged primates, small amounts of this peptide have been recovered from the brains of these animals.32 The uptake of peripheral Aß by the brain may be dependent on a compromised BBB, and aging and neurodegenerative diseases may contribute to BBB impairment.33 Disturbances in the BBB also occur in head trauma34 and vascular diseases such as atherosclerosis,35 hypertension,36 and stroke37 all of which have been found to represent risk factors for AD. Interestingly, Aß42 chronically infused into the circulation of rats, in which the BBB was breached, was localized in the brain parenchyma.38

In agreement with previous studies,39,40 a positive relationship between apoE {epsilon}4 and neuritic plaque density was also seen in our multiple regression analysis model. Our results support earlier findings that indicated a strong positive correlation between the concentration of brain Aß40, but not Aß42, and apoE {epsilon}4 allele dosage.41-43 As expected, the Braak stage strongly correlated with the neuritic plaque density. The brain Aß40 levels also correlated with the Braak stage. This trend, which reflects the gravity of the disease, is in agreement with recent observations demonstrating that the higher the level of Aß40 grows, the lower the level of synaptic density falls.23

Our finding of Aß peptides ending in residues 44, 45, and 46 by mass spectrometry in the temporalis muscles suggests that the site of {gamma}-secretase cleavage could be closer to the cytosolic leaflet of the membrane. This possibility was previously suggested by cell culture studies using mutated forms of Aß.44 It also suggests that there are multiple sites for {gamma}-secretase hydrolysis.45 Alternatively, {gamma}-secretase hydrolysis may only occur at residue 46, with the Aß thereafter shortened by carboxypeptidases to peptides ending in residues 45 and 44 and more frequently terminating at residues 42 and 40. The potential significance of the cleavage of Aß at residue 46, which corresponds to residue 717 of the AßPP770, is underscored by mutations at this position that lead to AD.46 The degradation of the C terminus of Aß by carboxypeptidases deserves further investigation in view of the apparent effectiveness by which the N terminus of Aß is degraded by proteolytic enzymes.24

A long-standing question in AD is why Aß does not accumulate in tissues other than the brain, despite the fact that several peripheral tissues express AßPP. Our efforts to immunocytochemically detect deposits of Aß in muscle met with frustration. This is not surprising, because the Aß peptide is promptly sequestered by a large number of proteins such as albumin that are present in high quantities in plasma and in the extracellular spaces of the body. We and others have recently found that albumin readily interacts with soluble Aß, masking its antigenic determinants and, in addition, inhibiting Aß fibrillogenesis.21,47,48 Histological examination of temporalis sections stained by H&E only demonstrated mild nonspecific myopathic changes consistent with normal aging. A more detailed and extensive histological investigation of the temporalis muscle is underway.

In summary, the temporalis muscles of AD individuals on the average contain significantly higher levels of Aß relative to the nondemented group. This suggests that the skeletal muscle may participate as a contributor to the plasma Aß pool as well as to the amyloid deposits of the brain and its vasculature observed in AD. The elevated levels of Aß in the temporalis muscles suggest that alterations in AßPP metabolism might be a systemic problem in AD and not a feature unique to the central nervous system.


   Acknowledgements
 
This paper is dedicated to the memory of our dear colleague W. Harold Civin, MD.


   Footnotes
 
Address reprint requests to Dr. Alex E. Roher, Haldeman Laboratory for Alzheimer Disease Research, Sun Health Research Institute, 10515 West Santa Fe Dr., Sun City, AZ 85351. E-mail: aroher{at}mail.sunhealth.org

Supported in part by the State of Arizona Center for Alzheimer’s Disease Research.

Accepted for publication November 16, 1999.


   References
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 

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