help button home button Am J Pathol International Conference on Pathology of Chest Diseases
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, H.
Right arrow Articles by Tsou, H. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, H.
Right arrow Articles by Tsou, H. C.
(American Journal of Pathology. 2001;158:381-385.)
© 2001 American Society for Investigative Pathology

Short Communication

Role of PTCH and p53 Genes in Early-Onset Basal Cell Carcinoma

Hong Zhang*, Xiao Li Ping*, Patricia K. Lee*, Xiu Li Wu*, Ya Juan Yao*, Ming Jian Zhang*, David N. Silvers*, Désirée Ratner*, Rajwant Malhotra{dagger}, Monica Peacocke* and Hui C. Tsou*{dagger}

From the Department of Dermatology,*
Columbia University, College of Physicians and Surgeons, New York, New York; and the Department of Pathology,{dagger}
University of Massachusetts Medical School, Worcester, Massachusetts


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 References
 
Basal cell carcinoma (BCC) is the most common skin cancer in the Western world. Ultraviolet (UV) exposure, race, age, gender, and decreased DNA repair capacity are known risk factors for the development of BCC. Of these, UVB irradiation from sunlight is the most significant risk factor. The incidence of sporadic BCC increases in individuals older than age 55, with the greatest incidence reported in individuals who are older than 70, and is rare in individuals who are younger than 30. In this study, we analyzed 24 BCC samples from individuals who had BCC diagnosed by the age of 30. Fifteen single-stranded conformation polymorphism variants in the PTCH gene were identified in 13 BCC samples. Sequence analysis of these single-stranded conformation polymorphism variants revealed 13 single nucleotide changes, one AT insertion, and one 15-bp deletion. Most of these nucleotide changes (nine of 15) were predicted to result in truncated PTCH proteins. Fifteen p53 mutations were also found in 11 of the 24 BCC samples. Thirty-three percent (five of 15) and 60% (nine of 15) of the nucleotide changes in the PTCH and p53 genes, respectively, were UV-specific C->T and CC->TT nucleotide changes. Our data demonstrate that the p53 and PTCH genes are both implicated in the development of early-onset BCC. The identification of UV-specific nucleotide changes in both tumor suppressor genes suggests that UV exposure is an important risk factor in early onset of BCC.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 References
 
Basal cell carcinoma (BCC) is the most common skin cancer in the Western world.1,2 It is classified, together with squamous cell carcinoma, as nonmelanoma skin cancer. BCC represents 75% of all nonmelanoma skin cancer. Known risk factors that contribute to the development of BCC include ultraviolet (UV) exposure, race, age, gender, and DNA repair capacity.3 UVB irradiation, from sunlight, is thought to be the major factor responsible for the development of BCCs, producing DNA damage at those sites where the pyrimidine of the base pair is part of a dipyrimidine sequence. C->T transitional changes at pyrimidine sites, including CC->TT double-base changes, are the most frequent form of nucleotide base substitution at the UVB-damaged dipyrimidine sites.

p53 mutations have been shown in 30 to 50% of BCCs studied, and more than half of these mutations were UV-specific C->T or CC->TT changes.4-9 These UV-specific changes in the p53 gene have also been detected in DNA from normal, sun-exposed skin.10,11 The human homologue of the Drosophila patched gene, PTCH, was first isolated by two independent groups during their search for the gene responsible for Nevoid basal cell carcinoma syndrome.12,13 Sequence analysis of DNA from Nevoid basal cell carcinoma syndrome individuals showed a series of germline mutations in the PTCH gene. Subsequently, somatic mutations in the PTCH gene were identified in 20 to 30% of the sporadic BCCs studied.12-18 Mutations detected in the PTCH genes from sporadic BCCs also contained UV-specific C->T and CC->TT nucleotide changes. Most of the PTCH mutations detected have been nonsense mutations, deletions, and insertions that lead to a premature termination of PTCH proteins.17

An individual’s DNA repair ability is thought to play a role in the development of BCC. A rare inherited disorder, xeroderma pigmentosum (XP), provides a human model that underscores the important role of DNA repair in preventing human cancers. Individuals with XP are unable to repair DNA damage19 and are 2,000 times more likely than normal individuals to develop sunlight-related BCC at an early age.20 Recently, a group studying 22 BCCs from patients with XP identified high levels (>60%) of UV-specific mutations in the PTCH gene.21 Epidemiological studies have also shown that individuals with BCC have a decreased ability to repair UV-induced DNA damage compared to control individuals without BCC.22

The incidence of sporadic BCCs increases in individuals older than age 55, with the greatest incidence reported in individuals who are older than 70 years old. BCC is rare in individuals who are younger than 30 years old. In a study conducted in a defined population in a city in southern Sweden, only 12 of 249 (4.8%) BCC cases were from individuals younger than 30 years old.23 Although some of these patients may have had a history of significant UV exposure, the development of BCCs at this early age is unusual. We postulated that mutations in the PTCH and p53 genes might have contributed to the development of skin cancers in this young population. To test that notion, we retrieved 24 paraffin blocks from 24 early onset BCC cases. Genomic DNA from these BCC samples was subjected to mutation analysis of the tumor suppressor genes PTCH and p53.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 References
 
Study Participants

Biopsy specimens with a confirmed pathological diagnosis of BCC from individuals who were younger than 30 years old at the time of biopsy were obtained from the Dermatopathology Laboratory at Columbia University. Each individual’s age, ethnic background, and medical history were reviewed by their dermatologist and none of these individuals has Nevoid basal cell carcinoma syndrome. The study was approved by the Columbia-Presbyterian Medical Center Institutional Review Board.

DNA Extraction

Paraffin sections containing >50% tumor tissue were placed into a 1.5-ml microcentrifuge tube and washed with xylene (three times for 30 minutes each). The sections were then digested in a buffer with proteinase K (provided in the QiAamp tissue kit; Qiagen, Valencia, CA) at 55°C overnight. The genomic DNA was then extracted following the instructions and columns provided by the QiAamp Tissue Kit from Qiagen. Genomic DNA of the peripheral blood from 20 normal individuals was used as normal control.

Single-Stranded Conformation Polymorphism (SSCP) Analysis

SSCP-polymerase chain reaction (PCR) reaction mixtures containing 25 ng of each primer, 22.5 µl of platinum PCR supermix (Life Technologies, Inc., Rockville, MD) and 0.5 µl of [33P]dCTP (Dupont-NEN, Boston, MA) were subjected to 30 cycles of PCR amplification. After thermal cycling, 1 µl of PCR product was added to 10 µl of stop solution (95% formamide, 10 mmol/L NaOH, 0.25% bromophenol blue, and 0.25% xylene cyanol). The mixtures were heated to 94°C for 3 minutes and placed on ice immediately. Three microliters of the denatured mixtures were loaded onto a 0.5x mutation detection enhancement gel (FMC Bioproducts, Rockland, ME) with 10% glycerol and run at 10 W for 20 hours at room temperature. PCR products with SSCP variants were sequenced using a BigDye terminator cycle sequencing kit (ABI) and were then run on an Applied Biosystems (Foster City, CA) 310 automated sequencing system.

Mutation Screening for the PTCH Gene

A set of 20 pairs of primers flanking exon 3 to exon 23 of the PTCH gene, as previously described,13,24 was used to amplify tumor genomic DNA. These PCR amplicons were then subjected to a SSCP-PCR reaction with the nested primers. All mutations were confirmed by new PCR and sequence reactions starting from DNA.

Mutation Screening for the p53 Gene

Every tumor sample was screened for mutations in the exon 4 to exon 8 of the p53 gene by direct sequencing analysis. Genomic DNA from each case was subjected to PCR amplification with primers flanking each exon of the p53 gene. The resulting amplicons were then sequenced and analyzed on an Applied Biosystems model 310 DNA sequencer. Table 1 lists the p53 primers used. Mutations were confirmed by new PCR and sequence reactions.


View this table:
[in this window]
[in a new window]
  		Table 1. Primers for the p53 Gene

 

   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 References
 
In this study, we examined 24 cases of BCC from individuals with a confirmed diagnosis of BCC before the age of 30. All individuals were Caucasians and ranged in age from 16 to 29 years (Table 2). Fifty-four percent (13 of 24) of the BCC samples were from the face and neck (the most common sites for sporadic BCC), and the remaining samples were from the trunk.

Genomic DNA isolated from each BCC sample was first subjected to a PCR-SSCP screening for mutations in the PTCH gene. A total of 15 SSCP variants were detected in 13 BCC samples. Sequence analysis of PCR products containing the SSCP variants revealed 15 sequence alternations spanning the entire PTCH gene. Twelve of the 15 sequence alternations were single nucleotide changes, resulting in six nonsense mutations, five missense mutations, and one silent mutation. In addition, two frameshift mutations and one in-frame mutation were detected. These consisted of a splice site mutation in exon 13, an AT insertion in exon 17, and a 15-bp deletion in exon 15, respectively. These three mutations, together with the six nonsense mutations, give rise to truncated PTCH proteins (Table 2).

Five missense mutations were detected spanning exons 4 to 21 of the coding sequences of the PTCH gene (Table 2 and Figure 1 ). Four of these five missense mutations were in or near transmembrane domains. Eleven of the 12 single nucleotide changes detected occurred at the dipyrimidine sites. Of 12 single nucleotide changes, five C->T and five G->A transitional changes were detected. They were consistent with UV-induced sunlight damage. We also detected a C->T transitional change at nucleotide 2004 in exon 14, resulting in a silent mutation (tyrosine to tyrosine substitution at codon 668). All five missense mutations and the one silent mutation were not detected in 40 normal control alleles, suggesting that they were not sequence polymorphisms.



View larger version (34K):
[in this window]
[in a new window]
  		Figure 1. Identification of missense mutations in the PTCH and p53 genes from BCC11 (no. 44). SSCP analysis showed a shifted band in PCR product encoding exon 5 of the PTCH gene from sample no. 44 (BCC11). Sequence analysis of this PCR product revealed a G->A change at nucleotide 709 that resulted in a glutamic acid to lysine substitution at codon 237. Direct sequence analysis of the p53 gene in the same BCC sample revealed an UV-specific, C->T transitional change at nucleotide 292 that resulted in a proline to serine substitution at codon 98. NL, wild-type sequence from normal tissue.

 
Sequence analysis from exon 4 to exon 8 of the p53 gene, was then performed on DNA from the same 24 BCC samples. Fifteen sequence alterations were identified in the coding sequences of the p53 gene from 11 BCC samples. Three BCC samples had two p53 mutations each. Twelve of the 15 sequence alterations were single and tandem nucleotide changes that resulted in 11 missense mutations and one nonsense mutation. Nine of the 12 single nucleotide changes were UV-specific C->T and CC->TT changes (Table 2 and Figure 1 ). Two 8-bp deletions in exon 6 and a 1-bp deletion in exon 8 of the p53 gene were detected in three BCC samples (Table 2). Nine of the 11 BCC samples with p53 mutations also contained PTCH mutations and all nine of these BCC samples contained UV-specific nucleotide changes in one or both tumor suppressor genes (Table 2).


   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
References
 
Sporadic BCC has the highest incidence in white males between the ages of 65 and 80. In this study, we evaluated a group of individuals who had their first BCC diagnosed before the age of 30. Because the incidence of sporadic BCC in this age group is very low, few studies have focused on this patient population. We screened 24 BCC samples from 24 individuals for mutations in the PTCH and p53 genes. All 24 individuals were Caucasians between the ages of 16 to 29. The site distribution of the BCC was 54% on the face and neck, and 46% on the trunk. This group of younger individuals has a lower frequency of BCC of the face and neck than the older individuals; >80% of the BCCs reported in the 65 to 80 age group are located on the face and neck.25 Moreover, >79% (19 of 24) of individuals in our early-onset group were female, in contrast to the older age group where the gender distribution is approximately equal.26

Fifty-four percent (13 of 24) of the BCC samples in this study had mutations in the PTCH gene, compared to an average PTCH mutation rate of 35% in BCC samples from older populations.13-15,17,18 The difference is statistically significant by chi-square test (P < 0.05). Of the 15 nucleotide changes in the PTCH gene, 80% (12 of 15) were point mutations. Our results differ from those reported in published studies on sporadic BCC in older age groups,14 where 47% (nine of 19) of the nucleotide changes were point mutations. Our data are similar to that of a recent study in BCC from individuals with XP. In that study, 73% (16 of 22) of BCC samples contained PTCH mutations, and 89% of them were point mutations.21 It has been suggested that decreased DNA repair ability in XP individuals contributes to the high frequency of PTCH mutations and high level of point mutations.21 Based on our findings, we speculate that early onset BCC results from a reduced ability to repair DNA damage. Using individuals without BCC as normal controls, Wei and colleagues22 showed that a combination of reduced DNA repair ability and exposure to UV irradiation was associated with an increased risk of BCC.

The PTCH gene encodes two large extracellular loops and 12 transmembrane domains that binds to sonic hehedgehog, a member of the hedgehog gene family.27 The two large extracellular domains of the PTCH gene are required for this binding.29 In this study, four of the six sequence alterations that led to five missense mutations and one silent mutation, were located in the transmembrane domains. The remaining nine nucleotide changes that we observed are predicted to result in a truncated PTCH protein. The mutations seen in our population differ from those seen in Nevoid basal cell carcinoma syndrome individuals, in whom most of the PTCH germline mutations reported were insertions and deletions.28

In our group of BCC patients, we found 15 p53 mutations in 11 BCC samples. Sixty percent (nine of 15) of these mutations were UV-specific C->T and CC->TT changes. The increased incidence of point mutations in PTCH coupled with UV-specific mutations in p53 suggests that the young individuals in our patient population may have a decreased ability to repair UV-induced DNA damage. Overall, we showed that 37% (nine of 24) of our BCC samples had mutations in both PTCH and p53 genes. Three BCC samples had only PTCH mutations, and two BCC samples had only p53 mutations.

In conclusion, we have found a series of mutations in the PTCH and p53 genes in BCC samples from a group of individuals with early onset BCC. We have shown that similar to previous studies in older age population, UV irradiation plays a major role in the development of BCC in a young population. We speculate that these young individuals have decreased DNA repair ability that renders them more susceptible to UV-induced DNA damage and, therefore, prone to develop BCC at a younger age. Further studies are needed to assess DNA repair ability in this population and to evaluate the role of other risk factors in the development of BCC in young individuals.


View this table:
[in this window]
[in a new window]
  		Table 2A. PTCH and p53 Mutations Identified in BCC Samples

 

View this table:
[in this window]
[in a new window]
  		Table 2B. BCC Samples with Wild-Type PTCH and p53 Genes

 

   Acknowledgements
 
We thank Dr. Michael J. Hutzler for his expert help on statistical analysis.


   Footnotes
 
Address reprint requests to Hui C. Tsou, M.D., Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, H2-468, Worcester, MA 01655. E-mail: hct5{at}columbia.edu

Supported by grants from the National Institute of Arthritis and Musculoskeletal and Skin Diseases (P&F project, PO-30 AR44535, to H. C. T.) and the National Institute of Aging (AG00760 to H. C. T. and AG00694 to M. P.).

Accepted for publication October 26, 2000.


   References
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 

  1. Miller SJ: Biology of basal cell carcinoma (part I). J Am Acad Dermatol 1991, 24:1-13[Medline]
  2. Gloster HM, Jr, Brodland DG: The epidemiology of skin cancer. Dermatol Surg 1996, 22:217-226[Medline]
  3. Preston DS, Stern RS: Nonmelanoma cancers of the skin. N Engl J Med 1992, 327:1649-1662[Medline]
  4. Rady P, Scinicariello F, Wagner RF Jr, Tyring SK: p53 mutations in basal cell carcinomas. Cancer Res 1992, 52:3804–3806
  5. Moles JP, Moyret C, Guillot B, Jeanteur P, Guilhou JJ, Theillet C, Basset-Seguin N: p53 gene mutations in human epithelial skin cancers. Oncogene 1993, 8:583-588[Medline]
  6. Ziegler A, Leffell DJ, Kunala S, Sharma HW, Gailani M, Simon JA, Halperin AJ, Baden HP, Shapiro PE, Bale AE, Brash DE: Mutation hotspots due to sunlight in the p53 gene of nonmelanoma skin cancers. Proc Natl Acad Sci USA 1993, 90:4216-4220[Abstract/Free Full Text]
  7. van der Riet P, Karp D, Farmer E, Wei Q, Grossman L, Tokino K, Ruppert JM, Sidransky D: Progression of basal cell carcinoma through loss of chromosome 9q and inactivation of a single p53 allele. Cancer Res 1994, 54:25-27[Abstract/Free Full Text]
  8. Kubo Y, Urano Y, Yoshimoto K, Iwahana H, Fukuhara K, Arase S, Itakura M: p53 gene mutations in human skin cancers and precancerous lesions: comparison with immunohistochemical analysis. J Invest Dermatol 1994, 102:440-444[Medline]
  9. D’Errico M, Calcagnile AS, Corona R, Fucci M, Annessi G, Baliva G, Tosti ME, Pasquini P, Dogliotti E: p53 mutations and chromosome instability in basal cell carcinomas developed at an early or late age. Cancer Res 1997, 57:747-752[Abstract/Free Full Text]
  10. Kanjilal S, Strom SS, Clayman GL, Weber RS, el-Naggar AK, Kapur V, Cummings KK, Hill LA, Spitz MR, Kripke ML, Anauthaswamy HN: p53 mutations in nonmelanoma skin cancer of the head and neck: molecular evidence for field cancerization. Cancer Res 1995, 55:3604-3609[Abstract/Free Full Text]
  11. Ponten F, Berg C, Ahmadian A, Ren ZP, Nister M, Lundeberg J, Uhlen M, Ponten J: Molecular pathology in basal cell cancer with p53 as a genetic marker. Oncogene 1997, 15:1059-1067[Medline]
  12. Johnson RL, Rothman AL, Xie J, Goodrich LV, Bare JW, Bonifas JM, Quinn AG, Myers RM, Cox DR, Epstein EH, Jr, Scott MP: Human homolog of patched, a candidate gene for the basal cell nevus syndrome. Science 1996, 272:1668-1671[Abstract]
  13. Hahn H, Wicking C, Zaphiropoulous PG, Gailani MR, Shanley S, Chidambaram A, Vorechovsky I, Holmberg E, Unden AB, Gillies S, Negus K, Smyth I, Pressman C, Leffell DJ, Gerrard B, Goldstein AM, Dean M, Toftgard R, Chenevix-Trench G, Wainwright B, Bale AE: Mutations of the human homolog of Drosophila patched in the nevoid basal cell carcinoma syndrome. Cell 1996, 85:841-851[Medline]
  14. Gailani MR, Stahle-Backdahl M, Leffell DJ, Glynn M, Zaphiropoulos PG, Pressman C, Unden AB, Dean M, Brash DE, Bale AE, Toftgard R: The role of the human homologue of Drosophila patched in sporadic basal cell carcinomas. Nat Genet 1996, 14:78-81[Medline]
  15. Unden AB, Holmberg E, Lundh-Rozell B, Stahle-Backdahl M, Zaphiropoulos PG, Toftgard R, Vorechovsky I: Mutations in the human homologue of Drosophila "patched" (PTCH) in basal cell carcinomas and the Gorlin syndrome: different in vivo mechanisms of PTCH inactivation. Cancer Res 1996, 56:4562-4565[Abstract/Free Full Text]
  16. Chidambaram A, Goldstein AM, Gailani MR, Gerrard B, Bale SJ, DiGiovanna JJ, Bale AE, Dean M: Mutations in the human homologue of the Drosophila patched gene in Caucasian and African-American nevoid basal cell carcinoma syndrome patients. Cancer Res 1996, 56:4599-4601[Abstract/Free Full Text]
  17. Wolter M, Reifenberger J, Sommer C, Ruzicka T, Reifenberger G: Mutations in the human homologue of the Drosophila segment polarity gene patched (PTCH) in sporadic basal cell carcinomas of the skin and primitive neuroectodermal tumors of the central nervous system. Cancer Res 1997, 57:2581-2585[Abstract/Free Full Text]
  18. Aszterbaum M, Rothman A, Johnson RL, Fisher M, Xie J, Bonifas JM, Zhang X, Scott MP, Epstein EH, Jr: Identification of mutations in the human PATCHED gene in sporadic basal cell carcinomas and in patients with the basal cell nevus syndrome. J Invest Dermatol 1998, 110:885-888[Medline]
  19. Pawsey SA, Magnus IA, Ramsay CA, Benson PF, Giannelli F: Clinical, genetic and DNA repair studies on a consecutive series of patients with xeroderma pigmentosum. Q J Med 1979, 48:179-210[Abstract/Free Full Text]
  20. Robbins JH: Xeroderma pigmentosum. Defective DNA repair causes skin cancer and neurodegeneration [clinical conference]. JAMA 1988, 260:384-388[Medline]
  21. Bodak N, Queille S, Avril MF, Bouadjar B, Drougard C, Sarasin A, Daya-Grosjean L: High levels of patched gene mutations in basal-cell carcinomas from patients with xeroderma pigmentosum. Proc Natl Acad Sci USA 1999, 96:5117-5122[Abstract/Free Full Text]
  22. Wei Q, Matanoski GM, Farmer ER, Hedayati MA, Grossman L: DNA repair capacity for ultraviolet light-induced damage is reduced in peripheral lymphocytes from patients with basal cell carcinoma [see comments]. J Invest Dermatol 1995, 104:933-936[Medline]
  23. Dahl E, Aberg M, Rausing A, Rausing EL: Basal cell carcinoma. An epidemiologic study in a defined population. Cancer 1992, 70:104-108[Medline]
  24. Xie J, Johnson RL, Zhang X, Bare JW, Waldman FM, Cogen PH, Menon AG, Warren RS, Chen LC, Scott MP, Epstein EH, Jr: Mutations of the PATCHED gene in several types of sporadic extracutaneous tumors. Cancer Res 1997, 57:2369-2372[Abstract/Free Full Text]
  25. Franceschi S, Levi F, Randimbison L, La Vecchia C: Site distribution of different types of skin cancer: new aetiological clues. Int J Cancer 1996, 67:24-28[Medline]
  26. Bastiaens MT, Hoefnagel JJ, Bruijn JA, Westendorp RG, Vermeer BJ, Bouwes Bavinck JN: Differences in age, site distribution, and sex between nodular and superficial basal cell carcinoma indicate different types of tumors. J Invest Dermatol 1998, 110:880-884[Medline]
  27. Ingham PW: Transducing Hedgehog: the story so far. EMBO J 1998, 17:3505-3511[Medline]
  28. Wicking C, Shanley S, Smyth I, Gillies S, Negus K, Graham S, Suthers G, Haites N, Edwards M, Wainwright B, Chenevix-Trench G: Most germ-line mutations in the nevoid basal cell carcinoma syndrome lead to a premature termination of the PATCHED protein, and no genotype-phenotype correlations are evident. Am J Hum Genet 1997, 60:21-26[Medline]
  29. Marigo V, Davey RA, Zuo Y, Cunningham JM, Tabin CJ: Biochemical evidence that patched is the Hedgehog receptor. Nature 1996, 384:176-179[Medline]



This article has been cited by other articles:


Home page
 FASEB J.Home page
A. Besaratinia, S.-i. Kim, and G. P. Pfeifer
Rapid repair of UVA-induced oxidized purines and persistence of UVB-induced dipyrimidine lesions determine the mutagenicity of sunlight in mouse cells
FASEB J, July 1, 2008; 22(7): 2379 - 2392.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. J. Taverna, J.-L. Selam, and G. Slama
Association between a Protein Polymorphism in the Start Codon of the Vitamin D Receptor Gene and Severe Diabetic Retinopathy in C-Peptide-Negative Type 1 Diabetes
J. Clin. Endocrinol. Metab., August 1, 2005; 90(8): 4803 - 4808.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Mancuso, S. Pazzaglia, M. Tanori, H. Hahn, P. Merola, S. Rebessi, M. J. Atkinson, V. Di Majo, V. Covelli, and A. Saran
Basal Cell Carcinoma and Its Development: Insights from Radiation-Induced Tumors in Ptch1-Deficient Mice
Cancer Res., February 1, 2004; 64(3): 934 - 941.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
S. Ramachandran, A. A. Fryer, T. Lovatt, A. Smith, J. Lear, P. W. Jones, and R. C. Strange
The rate of increase in the numbers of primary sporadic basal cell carcinomas during follow up is associated with age at first presentation
Carcinogenesis, December 1, 2002; 23(12): 2051 - 2054.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, H.
Right arrow Articles by Tsou, H. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, H.
Right arrow Articles by Tsou, H. C.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS