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From the Neuro-Gastroenterology and Nutrition Unit,* Institut National de la Recherche Agronomique, Toulouse, France; the Department of Pharmacology and Therapeutics and Physiology and Biophysics,¶ Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada; the Department of Surgery and Physiology, University of California at San Francisco, San Francisco, California; and the R. W. Johnson Pharmaceutical Research Institute, Spring House, Pennsylvania
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Abstract |
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The discovery of this novel receptor family has highlighted a new role for proteinases, not only as degradative enzymes, but also as signaling molecules that can affect tissue functions via the PARs. This new role for proteinases and their receptors has been recently investigated in the area of inflammation.3 When injected in vivo into the rat paw, PAR-2 agonists produce all of the classical hallmarks of inflammation: pain, swelling, heat, and redness.6 Other studies have shown additional proinflammatory effects for PAR-2 activation,3,7 including vasodilatation;8 hypotension;8,9 edema;6,7 leukocyte rolling, adherence and extravasation;10 and cytokine production.11 Conversely, several studies also suggest a protective role for PAR-2 in the airways,12 the gastric or colonic mucosa,13 or after myocardial ischemia-reperfusion injury.14
PAR-2 is highly expressed in the gastrointestinal tract, where it is found in endothelial cells, colonic myocytes, enterocytes (both on basolateral and apical membranes), enteric neurons, terminals of mesenteric afferent nerves, and immune cells.15,16 However, it is unclear whether PAR-2 serves a proinflammatory or anti-inflammatory role in the gastrointestinal tract. Because proteinases able to activate PAR-2 are normally present in the intestinal lumen, and because their levels are elevated in inflammatory bowel diseases, we evaluated whether luminal proteinases can induce inflammation. We also evaluated the effects of luminal PAR-2 agonists on epithelial permeability and bacterial translocation. Because PAR-2 antagonists are not yet available, a key feature of our study was in the use of PAR-2-deficient mice (PAR-2-/-), in which the actions of PAR-2 agonists could be compared with their effects in wild-type (PAR-2+/+) animals. Here we describe, for the first time, a proinflammatory role for acute PAR-2 activation in the colon of mice, which is accompanied by increased epithelial permeability and resulting bacterial translocation. Moreover, we provide evidence that trypsin and tryptase in the lumen of mouse colon are able to induce inflammation.
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Materials and Methods |
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Male Swiss 3T3 and C57BL6 mice were obtained respectively from Harlan (Grannat, France) and Charles River Laboratories (Quebec, Canada). PAR-2-/- mice were provided by the Johnson & Johnson Pharmaceutical Research Institute (Spring House, PA). All of the animals were housed in a temperature-controlled room; food and water were provided ad libitum. The local Animal Care and Ethic Committees approved all experimental protocols.
Chemicals
Peptides (the selective PAR-2-AP SLIGRL-NH2 and the control peptide LRGILS-NH2 inactive on PAR-2), prepared by solid-phase synthesis, were obtained from the peptide synthesis facility of the Faculty of Medicine, University of Calgary. The composition and the purity of peptides were confirmed by high-pressure liquid chromatography analysis; mass spectrometry and amino acid analysis were used to verify peptide concentration. The PAR-2-AP SLIGRL-NH2 has been shown to be a selective PAR-2 agonist.17 Peptides were dissolved in 10% ethanol, 10% Tween 80, and 80% saline (0.9% NaCl). Trypsin from porcine pancreas (type IX, 16,700 U/mg of protein) was from Sigma Chemical Co. (St. Louis, MO) and St. Quentin (Fallavier, France). Tryptase (2.5 mU/µg protein) was purified from human lung.18 Trypsin and tryptase were diluted in saline.
Intracolonic Injections
Mice were fasted for 12 hours. Under light halothane anesthesia, a polyethylene catheter was inserted intrarectally to 3 to 4 cm from the anus. All compounds were administrated into the distal colon through the catheter at a maximum volume of 100 µl.
Assessment of Inflammation
At different times after the intracolonic administrations, mice were killed and distal colonic tissues were excised to assess macroscopic damage using the criteria listed in Table 1
(adapted from previously described scoring systems).19
The bowel wall thickness was measured with a caliper at 1-cm from the anus. Myeloperoxidase (MPO) activity, an index of tissue granulocyte infiltration, was assayed in tissues as described.20,21
Other tissues from adjacent sites were fixed in neutral buffered formalin and processed by routine techniques for histological evaluation of microscopic signs of inflammation. Samples of colonic tissues were used for RNA isolation and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis.
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For PAR-2 RT-PCR, total RNA from mouse colonic tissues was isolated using the Trizol method (Gibco Canada). RNA (2 µg) was reverse-transcribed and DNA was amplified according to the following cycle conditions: dissociation of nucleic strands at 94°C for 1 minute, annealing at 55°C for 30 seconds, and extension for 1 minute at 72°C. The primer sequences for PAR-2 were: 5'-CAA GGT GCT CAT TGG CTT TT, 3'-CAG AGG GCG ACA AGG TAG AG, and for GADPH: 5'-CGG AGT CAA CGG ATT TGG TCG TAT, 3'-AGC CTT CTC CAT GGT GGT GAA GAC. Twenty-seven cycles were performed for PAR-2 and 23 cycles for GADPH. PCR products were then separated on a 1% agarose gel with ethidium bromide, the gel was scanned under UV light and bands quantified using a gel-doc system. For RT-PCR detection of cytokine mRNA, total mRNA (5 to 10 µg) was reverse-transcripted into complementary DNA (cDNA) using Superscript II RNase H- RT (Gibco-BRL, Cergy Pontoise, France). The primers used were ß-actin: 5'-GGG TCA GAA GGA TTC CTA TG-3' and 5'-GGT CTC AAA CAT GAT CTG GG-3'; interleukin (IL)-10: 5'-ATG CAG GAC TTT AAG GGT TACT TG-3' and 5'-AGA CAG CTT GGT CTT GGA GCT TA-3'; IL-4: 5'-TCG ACA TTT TGA ACG AGG TC-3' and 5'-GAA AAG CCC GAA AGA GTC TC-3'; interferon-
5'-GCT CTG AGA CAA TGA ACG CT-3' and 5'-AAA GAG ATA ATC TGG CTC TGC-3'; tumor necrosis factor-
5'-TCT CAT CAG TTC TAT GGC CC-3' and 5'-GGG AGT AGA CAA GGT ACA AC-3'; IL-1-ß 5'-AGA AGG TGC TCA TGT CCT CAT-3' and 5'-TTG ACG GAC CCC AAA AGA TG-3'. Competitive PCR analysis was performed using linearized plasmids: pQB3 for the competition with ß-actin, and pMus3 for the competition with interferon-, IL-2, IL-4, IL-5, IL-10, and tumor necrosis factor-.22,23
Amplification was performed for 40 cycles consisting of denaturation for 1 minute at 94°C, primer annealing for 1 minute at 52 to 55°C, and primer extension for 1.5 minutes at 72°C. DNA products were then separated on a 3% agarose gel with ethidium bromide and the ratio between amplified molecules for the target cDNA and the competitor, ie, log (DNA competitor/target cDNA), was calculated for each graded concentration of the competitor, using an image analyzer (QuantityOne software; Biorad, Amersham Pharmacia, Orsay, France). A curve of the ratios was established according to the competitor concentrations. This allowed the calculation of the equivalence point, at which the amount of amplified target mRNA and DNA competitor are equal [log (DNA competitor/target cDNA) = 0]. This value corresponds to the concentration of the target cDNA present in the initial sample.22,23
To quantify more accurately cytokines in samples, the number of cytokine molecules was expressed as compared with the number of cDNA molecules of the internal control ß-actin in the same sample.
Assessment of in Vivo Colonic Paracellular Intestinal Permeability
Anesthetized mice were treated intracolonically with SLIGRL-NH2 or LRGILS-NH2 (100 µg/mouse) and were then perfused intracolonically with51 Cr-ethylenediaminetetraacetic acid (EDTA) at 2.106 cpm/hour for 3 hours starting either immediately after PAR-2-AP (0 to 3 hours) or 3 hours after PAR-2-AP (3 to 6 hours) (in a total volume of 75 µl). After the 3-hour intracolonic perfusion period, blood was collected by cardiac puncture, and then measured for counts using a gamma counter.
Bacterial Translocation
Twenty-four hours after the intracolonic administrations, mice were killed by cervical dislocation and their organs were tested for translocated bacteria as previously described.24 Briefly, using sterile techniques, blood was obtained by cardiac puncture, and mesenteric lymph nodes, spleen, and liver were removed and weighed. The organs were homogenized and serial dilutions of aliquots (0.1 ml) were plated onto blood agar to enumerate total aerobic and facultative bacteria and plated onto MacConkey’s agar to enumerate aerobic and facultative gram-negative enteric bacilli. The plates were incubated for 24 and 48 hours at 37°C in aerobic conditions and the number of bacterial colonies was recorded.
Immunohistochemistry
Mice were infused intracolonically with PAR-2-AP, the control peptide (100 µg/mouse each), or their vehicle, and 4, 6, and 10 hours later, tissues were harvested and processed for immunohistochemistry.25
Frozen sections were washed with phosphate-buffered saline (PBS) containing 1% normal goat serum and 0.3% Triton X-100 for 10 minutes, and were preblocked with PBS containing 5% normal goat serum and 0.3% Triton X-100 for 30 minutes. Sections were incubated with primary antibody in PBS with 5% normal goat serum and 0.3% Triton X-100 for 24 hours at 4°C. Primary antibody B5 was raised to rat PAR-2 (30GPNSKGR
SLIGRLDT46PYGGC, = cleavage site) conjugated to keyhole limpet hemocyanin, and was used at a 1:250 to 1:500 dilution. In control experiments, the B5 antibody was preincubated for 24 to 48 hours at 4°C with 1 or 10 µmol/L of the peptide used for immunization before staining, or was omitted. Slides were washed and incubated with secondary antibody conjugated to fluorescein isothiocyanate (1:200 dilution; Jackson ImmunoResearch, West Grove, PA) for 2 hours at room temperature. Tissue sections were examined using an MRC 1000 laser-scanning confocal microscope (Bio-Rad, Hercules, CA) equipped with a krypton/argon laser and attached to a Zeiss Axiovert microscope. Images were processed using Adobe Photoshop.
Bacterial Translocation
Twenty-four hours after the intracolonic administration of the PAR-2-AP or the control peptide (100 µg/mouse each), mice were killed by cervical dislocation and their organs were tested for translocated bacteria as previously described.24 Briefly, using sterile techniques, blood was obtained by cardiac puncture, and mesenteric lymph nodes, spleen, and liver were removed and weighed. The organs were homogenized, and serial dilutions of aliquots (0.1 ml) were plated onto blood agar to enumerate total aerobic and facultative bacteria and plated onto MacConkey’s agar to enumerate aerobic and facultative gram-negative enteric bacilli. The plates were incubated for 24 and 48 hours at 37°C in aerobic conditions and the number of bacterial colonies was recorded.
Data Analysis
For all groups, significance was estimated using the appropriate version of Student’s t-test. Group data are expressed as mean ± SE, and a P value <0.05 was required to reject the null hypothesis.
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Results |
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A PCR product of a predicted size of 549 bp was amplified from RNA prepared from the colons of mice, showing PAR-2 presence in those tissues. Compared to the level of expression of PAR-2 after intracolonic injection of the control peptide LRGILS-NH2 (100 µg/mouse), administration of the selective PAR-2 agonist SLIGRL-NH2 (100 µg/mouse) caused a significant increase in PAR-2 mRNA expression relative to the GAPDH RT-PCR signal (size, 306 bp) 10 hours after the peptide injection (Figure 1, A and B)
. PAR-2 immunoreactivity was prominently localized to colonocytes where it was detected at the membrane and in intracellular compartments, as previously described.25,26
Four hours after SLIGRL-NH2 injection, PAR-2 immunoreactivity markedly diminished in both villi and crypts, while 6 and 10 hours later, PAR-2 was up-regulated and was found present prominently in intracellular compartments in crypts. Staining was abolished by preabsorption of antibody with the receptor fragments or omission of primary antibody (Figure 1C)
. These results show that PAR-2 activation results in down-regulation of PAR-2, possibly because of endocytosis and degradation, followed by up-regulation of mRNA and protein reflecting increase of exocytosis.
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PAR-2-AP
The intracolonic administration of SLIGRL-NH2 (100 µg/mouse) caused a significant increase in macroscopic damage score, wall thickness, and MPO activity in colonic tissues from C57BL6 mice (Figure 2; A, B, and C)
. A maximal effect was observed from 4 to 6 hours after SLIGRL-NH2 dosing (Figure 2; A, B, and C)
. At all of the observed time points, the same dose of the control peptide LRGILS-NH2 had no effect on the observed inflammatory parameters. Histological examination revealed edema in the submucosa (Figure 3C
, arrowheads) and erosion of the epithelium (Figure 3C
, arrows) after SLIGRL-NH2 in wild-type mice but not in PAR-2-/- mice (Figure 3D)
, whereas LRGILS-NH2 did not cause significant damage compared to saline (Figure 3, B and A)
. In another mouse strain (Swiss 3T3 mice), SLIGRL-NH2 also caused a significant increase in MPO activity and microscopic damage score, with a maximum effect 4 hours after peptide intracolonic administration (Figure 2, D and E)
. As per the C57BL strain, the effect of SLIGRL-NH2 on MPO activity and microscopic damage score in Swiss 3T3 mice was dose-dependent from 10 µg to 100 µg/mouse, and the control peptide had no effect (Figure 2, F and G)
.
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Trypsin (400 U/mouse) or human mast cell tryptase (1 µg/mouse) caused increased macroscopic damage score (70 to 90%), wall thickness (32 to 50%), and MPO activity (65 to 84%) in colonic tissues from C57BL6 mice 6 hours after their intracolonic administration (Figure 4; A, B, and C)
. The maximum effect of trypsin and tryptase was observed between 4 and 6 hours (time course not shown), whereas the inactive enzymes (boiled for 10 minutes) had no effect (Figure 4; A, B, and C)
at all time points. In Swiss 3T3 mice, trypsin also increased MPO activity and microscopic damage score (not shown), in a dose-dependent manner, from 10 to 400 U/mouse, 4 hours after its colonic administration (Figure 4D)
. These results show that luminal administration of PAR-2 agonists (peptides and proteinases) induces inflammation in the colon of mice.
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Four hours after its intracolonic administration, SLIGRL-NH2 (100 µg/mouse), but not the control peptide LRGILS-NH2, caused a significant increase in tumor necrosis factor-, IL-1ß, and interferon- mRNA expression in Swiss 3T3 mouse colonic tissues, whereas the expression of IL-4 and IL-10 remained unchanged (Figure 5)
. Thus PAR-2 agonists induce elevation of T-helper cell type 1 (Th1) cytokines in the mouse colon.
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Intracolonic administration of SLIGRL-NH2, but not LRGILS-NH2 (100 µg/mouse each), significantly increased the passage of the permeability marker 51Cr-EDTA from the gut lumen to the vasculature. Permeability was unchanged between 0 and 3 hours after the peptide intracolonic administration, but was substantially increased between 3 and 6 hours after SLIGRL-NH2 dosing (Figure 6)
. In contrast to LRGILS-NH2-injected mice, whose organs were sterile 24 hours after peptide treatment, bacteria translocated from the gut to the mesenteric lymph nodes, but also to the liver, spleen, and blood, after the intracolonic administration of SLIGRL-NH2 (100 µg/mouse) (Table 2)
. Thus, PAR-2 agonists induce increased colonic paracellular permeability and bacterial translocation.
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To investigate whether the PAR-2 agonist-induced colonic inflammation was because of PAR-2 activation, we used mice deficient for the PAR-2 gene, compared to the C57BL wild-type mice. When injected into the colon of PAR-2-/- mice, SLIGRL-NH2 did not cause increased macroscopic damage score, wall thickness, or MPO activity, as observed in C57BL wild-type mice (Figure 7; A, B, and C)
. Further, no elevated mRNA levels of Th1 cytokine interferon- or inflammatory cytokine tumor necrosis factor- were observed in PAR-2-deficient mice compared with C57BL wild-type mice after the intracolonic administration of SLIGRL-NH2 (Figure 7D)
. Finally, SLIGRL-NH2 did not cause bacterial translocation (Table 2)
or increased permeability to 51Cr-EDTA in PAR-2-deficient mice, compared to wild-type mice (Figure 7E)
. The intracolonic administration of trypsin or tryptase in PAR-2-deficient mice did not cause changes in macroscopic damage score, wall thickness, or MPO activity (Figure 8; A, B, and C)
, confirming that the trypsin and tryptase-induced inflammatory parameters were because of PAR-2 activation. Thus, the PAR-2 agonists SLIGRL-NH2, trypsin and tryptase, induce intestinal inflammation and cause increased intestinal permeability by activation of PAR-2.
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SLIGRL-NH2 (100 µg/mouse), when injected intraperitoneally, did not induce change in both microscopic damage scores and MPO activities at 2, 4, 6, 8, or 10 hours after the peptide injection (Table 3A)
. Higher intraperitoneal doses of SLIGRL-NH2 (up to 400 µg/mice) did not cause significant changes in MPO activity and microscopic damage 4 hours after its injection (time point corresponding to the maximum effect of SLIGRL-NH2 intracolonic administration) (Table 3B)
. This result shows that systemic administration of PAR-2-AP did not induce colon inflammation.
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Discussion |
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Luminal PAR-2 Agonists Induce Colonic Inflammation and Loss of Barrier Function
Our results showed, in two different strains of mice, that PAR-2 agonists present in the colonic lumen can provoke an inflammatory reaction characterized by granulocyte infiltration (elevated levels of MPO activity), tissue damage, elevated cytokine expression, and also caused changes in intestinal permeability and subsequent bacterial translocation to peritoneal organs. We confirmed that the proinflammatory effects of PAR-2 agonists were mediated by PAR-2 and not by a related receptor, observing the lack of effects of PAR-2-AP in mice lacking the gene that encodes PAR-2 (Figure 7
and Table 2
). The elevated expression of PAR-2 mRNA and the internalization of the receptor in colonic tissues 10 hours after exposure to SLIGRL-NH2 support further the hypothesis that PAR-2 is a key factor in the pathophysiology of the changes observed (Figure 1)
. The deleterious effects we observed for PAR-2 activation in the mouse colon constitute a first body of evidence to suggest an active role for PAR-2 in the pathophysiological changes observed in intestinal inflammatory diseases.
Cells Involved in PAR-2 Agonist-Induced Inflammation and Permeability Breakage
Considering the increased intestinal permeability after PAR-2-AP intracolonic administration, it is possible that the peptide reaches the circulation and could potentially influence other organ systems. However, the lack of proinflammatory effects in the mouse colon after SLIGRL-NH2 intraperitoneal injection (Table 3)
supports a direct involvement of cells located on the colonic wall to induce intestinal inflammation. It has been shown that PAR-2 is expressed at the apical and basolateral membrane of rat and human enterocytes and our results confirmed the same presence of PAR-2 on mouse enterocytes (Figure 1B)
. The internalization of the receptor in enterocytes after exposure to SLIGRL-NH2 (Figure 1B)
, suggested that these cells are responsible, at least in part, for the PAR-2 agonist-induced intestinal changes. Moreover, it has been shown that PAR-2 agonists can signal to enterocytes, provoking the release of prostaglandin E2, arachidonic acid, and inositol 1,4,5-trisphosphate.26
It can be hypothesized that PAR-2-mediated intestinal barrier breakdown constitutes an initial event, which then allows pathogen penetration to cause further inflammation. However, the fact that inflammation preceded paracellular permeability in our study does not support this hypothesis and might actually favor the hypothesis that inflammation might be responsible for increased intestinal permeability as previously described.32
We cannot rule out a possible concomitant direct activation of PAR-2 on other cells present in the colon. PAR-2 expression has been described in cells implicated in inflammation such as neutrophils, eosinophils, endothelial cells, neurons, and fibroblasts, where its activation leads to proinflammatory signals (eg, cytokine expression, leukocyte rolling and adhesion, increased vascular permeability).3
Thus, it is also possible that SLIGRL-NH2-induced colonic inflammation might be mediated by a direct activation of PAR-2 on inflammation-related cells and the subsequent release of inflammatory mediators. These inflammatory mediators (ie, cytokines) might then modify paracellular permeability and junctional protein expression as it was observed in enterocytes exposed to interferon-.33
Clinical studies of inflammatory bowel disease patients suggest that the down-regulation of junctional molecules observed in ulcerative colitis34
or Crohn’s disease patients35
does not likely represent a primary phenomenon.36
These studies support the view that alterations in tight junction proteins and subsequent changes in paracellular permeability may be the consequence rather than the triggering factor of colonic mucosa inflammation.
Trypsin and Tryptase Are Responsible for PAR-2 Colonic Activation
One of the major obstacles in defining a physiological role for PAR-2 resides in the question of the endogenous proteinase responsible for its activation. Under physiological conditions, trypsin is present in the intestinal lumen after feeding, but mainly in the upper gastrointestinal tract. In the colon, trypsin does not exert any digestive functions and its activity is constantly counterbalanced by the presence of trypsin inhibitors. Although trypsin is not released in the intestine on inflammation, studies have shown that the abundance of trypsin inhibitors is substantially reduced in the tissues of patients with ulcerative colitis or Crohn’s disease37
and that trypsin proteolytic activity is increased in the intestinal lumen of these patients38
or in animal models of colitis.39
Tryptase, another proteinase that can activate PAR-2, constitutes the major protein released upon mucosal mast cell degranulation in humans, and is released in the setting of inflammation and allergic response into the intestinal lumen and vasculature.40
Thus, trypsin and tryptase constitute good candidates to activate PAR-2 in the intestinal lumen in pathophysiological conditions. Our study showed that trypsin and tryptase reproduced the proinflammatory effects of the selective PAR-2-AP when injected directly into the colon. Experiments using PAR-2-deficient mice have further implicated trypsin and tryptase as possible endogenous proteinases responsible for PAR-2 activation showing that the proinflammatory effects of trypsin and tryptase were entirely mediated by PAR-2 (Figure 8)
. Although our results showed that human tryptase induced colonic inflammation in mice through the activation of PAR-2, it is important to note that mucosal mast cells that are normally present in the mouse intestine lack tryptase.41
However, it has been shown in mice that jejunal mast cells can alter reversibly the expression of chymases or tryptases according to the inflammatory state after Trichinella spiralis infection.42
In physiological conditions, a constant balance between proteinases and their inhibitors is observed, both in the vasculature and the gut lumen, and this is particularly true for trypsin, which is primarily present in the small intestine. In pathophysiological conditions such as inflammatory bowel disease, allergy, or parasite infection, the physiological balance of proteinase activity in the gut lumen is broken. From our results, it seems that luminal PAR-2 activation rendered possible by an increased tryptic activity in the gut lumen could generate an inflammatory reaction. A very recent study has shown that treatment of ulcerative colitis patients with a tryptase inhibitor significantly improved (49% of the patients) or even caused remission (9% of the patients) of the disease.43
This clearly suggests that tryptase is involved in the generation of inflammatory symptoms associated with ulcerative colitis and from our results, we can think that tryptase involvement in the generation of inflammation might be mediated by the activation of PAR-2.
PAR-2 Pro- or Anti-Inflammatory Agent?
Although all of the classical hallmarks of inflammation, ie, swelling (increased wall thickness), redness (erythema), impaired function, and even pain,44
have been observed after the acute intracolonic administration of a selective PAR-2 agonist, it is also necessary to consider a possible anti-inflammatory role for PAR-2. Several studies have suggested that PAR-2 activation might promote resolution of inflammation.3
In airway epithelia, both PAR-1-AP and PAR-2-AP elicit relaxation through the release of prostaglandin E2, thus causing a powerful bronchodilatation.12
A recent study has shown that systemic treatment with PAR-2-AP inhibits the development of TNBS-induced colitis leading to an increased survival rate, improved macroscopic and histological damage score, and a decrease in the mucosal content of Th1 helper cell type 1 cytokines.13
In that study, the authors also showed that in vivo treatment with a PAR-2-AP directly inhibited trinitrobenzensulfonic acid-induced interferon- secretion and CD44 expression on lamina propria T lymphocytes.13
The apparent discrepancy between our results documenting a proinflammatory role for PAR-2-AP and anti-inflammatory effects of PAR-2-AP observed by Fiorucci and colleagues13
in the TNBS-induced colitis model, may be explained by the nature of inflammation. The TNBS-induced colitis model is a model of chronic intestinal inflammation and the authors have looked at the damage 7 days after the induction of colitis, whereas in our study, an acute exposure to PAR-2-AP provoked an intestinal inflammation that was maximal between 4 and 6 hours after its induction. It is well known that some inflammatory mediators provoke an inflammatory reaction when administered acutely, but can be anti-inflammatory in the setting of chronic inflammation.45-47
Another explanation for proinflammatory versus anti-inflammatory effects of PAR-2 activation is that inflammatory response may result from local activation of PAR-2, whereas systemic activation of PAR-2 may lead to an anti-inflammatory effect. In fact, local activation of PAR-2 (intracolonic administration), but not systemic injection (intraperitoneal) was responsible for the proinflammatory effects in the colon, whereas only a systemic injection of PAR-2 agonists induced resolution of TNBS-induced colitis.13
It has been established that the induction of hypotension can be responsible for a decreased inflammation in models such as carrageenan-induced paw inflammation,48
and systemic administration of PAR-2-AP is known to cause hypotension,8,49,50
it is thus possible that hypotension induced by a chronic and systemic treatment with PAR-2-AP is responsible for the anti-inflammatory effects observed in the model of TNBS-induced colitis. Systemic versus local administration of PAR-2-AP activates different target cells expressing PAR-2 and these cells might be implicated differently in the inflammatory cascade. For example, it has been shown that endothelial activation of PAR-2, which might occur after systemic injection of PAR-2-AP, reduced tissue damage induced by ischemia-reperfusion injuries14
and cardiac inflammation,51
whereas PAR-2 activation on epithelial cells has been shown to induce the release of proinflammatory cytokines such as IL-8 and IL-652
or the release of arachidonic acid, the precursor of lipid inflammatory mediators, from enterocytes.26
In summary, our study shows that a direct local activation of PAR-2 in the mouse colon leads to intestinal inflammation and a severe impairment of intestinal permeability. Trypsin and tryptase, through the activation of PAR-2, also causes colonic inflammation. Considering the large presence of those two proteinases in the gut, and the increased luminal tryptic activity associated with inflammatory bowel disease, it is likely that trypsin and/or tryptase can locally activate PAR-2, and might then participate in the intestinal pathophysiological changes observed in inflammatory bowel disease patients.
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Footnotes |
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Supported by grants from the Canadian Institute of Health Research (to N. V., J. L. W., M. D. H.), NicOx S.A. (to N. V.), the Canadian Association of Gastroenterology (to N. V., C. N.), Johnson & Johnson Focused Giving (to J. L. W., M. D. H., N. W. B.), the UPSA Foundation (to N. C., R. G. V., L. B.), the French Institut National de la Recherche Agronomique (to N. C., R. G. V., L. B.), Pfizer Laboratories Fresnes, France (to A. C.), and the National Institutes of Health (grants DK 43207, 57480, 39957 to N. W. B.).
N. C. is a Ministère de la Recherche (France) studentship recipient, and N. V. is a Canadian Institute of Health Research Scholar.
Accepted for publication August 1, 2002.
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L. Moussa, J. Apostolopoulos, P. Davenport, J. Tchongue, and P. G. Tipping Protease-Activated Receptor-2 Augments Experimental Crescentic Glomerulonephritis Am. J. Pathol., September 1, 2007; 171(3): 800 - 808. [Abstract] [Full Text] [PDF]
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R. Roka, A. Ait-Belgnaoui, C. Salvador-Cartier, R. Garcia-Villar, J. Fioramonti, H. Eutamene, and L. Bueno Dexamethasone prevents visceral hyperalgesia but not colonic permeability increase induced by luminal protease-activated receptor-2 agonist in rats Gut, August 1, 2007; 56(8): 1072 - 1078. [Abstract] [Full Text] [PDF]
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Y. Kida, H. Inoue, T. Shimizu, and K. Kuwano Serratia marcescens Serralysin Induces Inflammatory Responses through Protease-Activated Receptor 2 Infect. Immun., January 1, 2007; 75(1): 164 - 174. [Abstract] [Full Text] [PDF]
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 C A Dinarello and S-H Kim IL-32, a novel cytokine with a possible role in disease Ann Rheum Dis, November 1, 2006; 65(suppl_3): iii61 - iii64. [Abstract] [Full Text] [PDF]
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 S. Amadesi, G. S. Cottrell, L. Divino, K. Chapman, E. F. Grady, F. Bautista, R. Karanjia, C. Barajas-Lopez, S. Vanner, N. Vergnolle, et al. Protease-activated receptor 2 sensitizes TRPV1 by protein kinase C{varepsilon}- and A-dependent mechanisms in rats and mice J. Physiol., September 1, 2006; 575(2): 555 - 571. [Abstract] [Full Text] [PDF]
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F. Cattaruzza, N. Cenac, E. Barocelli, M. Impicciatore, E. Hyun, N. Vergnolle, and C. Sternini Protective Effect of Proteinase-Activated Receptor 2 Activation on Motility Impairment and Tissue Damage Induced by Intestinal Ischemia/Reperfusion in Rodents Am. J. Pathol., July 1, 2006; 169(1): 177 - 188. [Abstract] [Full Text] [PDF]
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J Demaude, C Salvador-Cartier, J Fioramonti, L Ferrier, and L Bueno Phenotypic changes in colonocytes following acute stress or activation of mast cells in mice: implications for delayed epithelial barrier dysfunction Gut, May 1, 2006; 55(5): 655 - 661. [Abstract] [Full Text] [PDF]
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 M. Chignard and D. Pidard Neutrophil and Pathogen Proteinases versus Proteinase-Activated Receptor-2 Lung Epithelial Cells: More Terminators than Activators. Am. J. Respir. Cell Mol. Biol., April 1, 2006; 34(4): 394 - 398. [Abstract] [Full Text] [PDF]
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M. Holzhausen, L. C. Spolidorio, R. P. Ellen, M.-C. Jobin, M. Steinhoff, P. Andrade-Gordon, and N. Vergnolle Protease-Activated Receptor-2 Activation: A Major Role in the Pathogenesis of Porphyromonas gingivalis Infection Am. J. Pathol., April 1, 2006; 168(4): 1189 - 1199. [Abstract] [Full Text] [PDF]
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 D. Novick, M. Rubinstein, T. Azam, A. Rabinkov, C. A. Dinarello, and S.-H. Kim Proteinase 3 is an IL-32 binding protein PNAS, February 28, 2006; 103(9): 3316 - 3321. [Abstract] [Full Text] [PDF]
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 F. Noorbakhsh, S. Tsutsui, N. Vergnolle, L. A. Boven, N. Shariat, M. Vodjgani, K. G. Warren, P. Andrade-Gordon, M. D. Hollenberg, and C. Power Proteinase-activated receptor 2 modulates neuroinflammation in experimental autoimmune encephalomyelitis and multiple sclerosis J. Exp. Med., February 21, 2006; 203(2): 425 - 435. [Abstract] [Full Text] [PDF]
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S. Swift, A. J. Leger, J. Talavera, L. Zhang, A. Bohm, and A. Kuliopulos Role of the PAR1 Receptor 8th Helix in Signaling: THE 7-8-1 RECEPTOR ACTIVATION MECHANISM J. Biol. Chem., February 17, 2006; 281(7): 4109 - 4116. [Abstract] [Full Text] [PDF]
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C. Jacob, P.-C. Yang, D. Darmoul, S. Amadesi, T. Saito, G. S. Cottrell, A.-M. Coelho, P. Singh, E. F. Grady, M. Perdue, et al. Mast Cell Tryptase Controls Paracellular Permeability of the Intestine: ROLE OF PROTEASE-ACTIVATED RECEPTOR 2 AND {beta}-ARRESTINS J. Biol. Chem., September 9, 2005; 280(36): 31936 - 31948. [Abstract] [Full Text] [PDF]
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K. K. Hansen, P. M. Sherman, L. Cellars, P. Andrade-Gordon, Z. Pan, A. Baruch, J. L. Wallace, M. D. Hollenberg, and N. Vergnolle A major role for proteolytic activity and proteinase-activated receptor-2 in the pathogenesis of infectious colitis PNAS, June 7, 2005; 102(23): 8363 - 8368. [Abstract] [Full Text] [PDF]
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N Vergnolle CLINICAL RELEVANCE OF PROTEINASE ACTIVATED RECEPTORS (PARS) IN THE GUT Gut, June 1, 2005; 54(6): 867 - 874. [Full Text] [PDF]
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 F. Noorbakhsh, N. Vergnolle, J. C. McArthur, C. Silva, M. Vodjgani, P. Andrade-Gordon, M. D. Hollenberg, and C. Power Proteinase-Activated Receptor-2 Induction by Neuroinflammation Prevents Neuronal Death during HIV Infection J. Immunol., June 1, 2005; 174(11): 7320 - 7329. [Abstract] [Full Text] [PDF]
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 M. Holzhausen, L.C. Spolidorio, and N. Vergnolle Proteinase-activated Receptor-2 (PAR2) Agonist Causes Periodontitis in Rats Journal of Dental Research, February 1, 2005; 84(2): 154 - 159. [Abstract] [Full Text] [PDF]
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 M. Steinhoff, J. Buddenkotte, V. Shpacovitch, A. Rattenholl, C. Moormann, N. Vergnolle, T. A. Luger, and M. D. Hollenberg Proteinase-Activated Receptors: Transducers of Proteinase-Mediated Signaling in Inflammation and Immune Response Endocr. Rev., February 1, 2005; 26(1): 1 - 43. [Abstract] [Full Text] [PDF]
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 A. Masamune, K. Kikuta, M. Satoh, N. Suzuki, and T. Shimosegawa Protease-Activated Receptor-2-Mediated Proliferation and Collagen Production of Rat Pancreatic Stellate Cells J. Pharmacol. Exp. Ther., February 1, 2005; 312(2): 651 - 658. [Abstract] [Full Text] [PDF]
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A. K Chan, N. Vergnolle, M. D Hollenberg, and P.-Y. von der Weid Proteinase-activated receptor 2 activation modulates guinea-pig mesenteric lymphatic vessel pacemaker potential and contractile activity J. Physiol., October 15, 2004; 560(2): 563 - 576. [Abstract] [Full Text] [PDF]
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N. Cenac, A. C. Chin, R. Garcia-Villar, C. Salvador-Cartier, L. Ferrier, N. Vergnolle, A. G. Buret, J. Fioramonti, and L. Bueno PAR2 activation alters colonic paracellular permeability in mice via IFN-{gamma}-dependent and -independent pathways J. Physiol., August 1, 2004; 558(3): 913 - 925. [Abstract] [Full Text] [PDF]
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A. Kawabata, T. Kanke, D. Yonezawa, T. Ishiki, M. Saka, M. Kabeya, F. Sekiguchi, S. Kubo, R. Kuroda, M. Iwaki, et al. Potent and Metabolically Stable Agonists for Protease-Activated Receptor-2: Evaluation of Activity in Multiple Assay Systems in Vitro and in Vivo J. Pharmacol. Exp. Ther., June 1, 2004; 309(3): 1098 - 1107. [Abstract] [Full Text] [PDF]
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 S. Amadesi, J. Nie, N. Vergnolle, G. S. Cottrell, E. F. Grady, M. Trevisani, C. Manni, P. Geppetti, J. A. McRoberts, H. Ennes, et al. Protease-Activated Receptor 2 Sensitizes the Capsaicin Receptor Transient Receptor Potential Vanilloid Receptor 1 to Induce Hyperalgesia J. Neurosci., May 5, 2004; 24(18): 4300 - 4312. [Abstract] [Full Text] [PDF]
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G. S. Cottrell, S. Amadesi, E. F. Grady, and N. W. Bunnett Trypsin IV, a Novel Agonist of Protease-activated Receptors 2 and 4 J. Biol. Chem., April 2, 2004; 279(14): 13532 - 13539. [Abstract] [Full Text] [PDF]
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 V. S. OSSOVSKAYA and N. W. BUNNETT Protease-Activated Receptors: Contribution to Physiology and Disease Physiol Rev, April 1, 2004; 84(2): 579 - 621. [Abstract] [Full Text] [PDF]
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A. Zhao and T. Shea-Donohue PAR-2 agonists induce contraction of murine small intestine through neurokinin receptors Am J Physiol Gastrointest Liver Physiol, October 1, 2003; 285(4): G696 - G703. [Abstract] [Full Text] [PDF]
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A. C. Chin, N. Vergnolle, W. K. MacNaughton, J. L. Wallace, M. D. Hollenberg, and A. G. Buret Proteinase-activated receptor 1 activation induces epithelial apoptosis and increases intestinal permeability PNAS, September 16, 2003; 100(19): 11104 - 11109. [Abstract] [Full Text] [PDF]
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N. Cenac, R. Garcia-Villar, L. Ferrier, M. Larauche, N. Vergnolle, N. W. Bunnett, A.-M. Coelho, J. Fioramonti, and L. Bueno Proteinase-Activated Receptor-2-Induced Colonic Inflammation in Mice: Possible Involvement of Afferent Neurons, Nitric Oxide, and Paracellular Permeability J. Immunol., April 15, 2003; 170(8): 4296 - 4300. [Abstract] [Full Text] [PDF]
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