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From the Gastrointestinal Research Group,* University of Calgary, Calgary, Canada; and the Gastrointestinal Unit,* Center for Study of Inflammatory Bowel Disease, Massachusetts General Hospital, Boston, Massachusetts
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These complexities have made the definition of the role of TGF-ß in the intestinal mucosa, and specifically, the intestinal epithelial cell especially challenging. The intestinal epithelium is a highly specialized cell population undergoing continuous rapid turnover. Most importantly, it is vulnerable to injury from many different processes including, drugs, radiation therapy, infectious agents, and inflammation associated with Crohn’s disease and ulcerative colitis. When injury occurs, rapid restoration of the continuity of the epithelial barrier and ultimately normal mucosal architecture is essential. Indeed TGF-ß has been found, in vitro, to exert potent effects on the intestinal epithelium, which appear to modulate these responses.7,8 However, some of these effects could be counterbalanced by opposing functional actions.4,9 TGF-ß is a potent inhibitor of intestinal epithelial cell proliferation. Indeed, inactivation of TGF-ß receptor II signaling has been described in many colorectal cancers and presumably contributes to lack of regulation of control of cell proliferation.10-12 In addition, TGF-ß stimulates production of extracellular matrix components including collagen, which may also facilitate repair but can ultimately contribute to fibrosis, a nonphysiological outcome of healing.4,13-15 Furthermore, TGF-ß has potent effects on the many other cell populations present in the intestinal mucosa that could dominate the overall outcome of mucosal injury including lymphocytes, macrophages, fibroblasts, and when present, neutrophils.
To determine the functional significance of TGF-ß in the intestinal epithelial cell compartment, we have selectively expressed a truncated TGF-ß RII in this cell compartment. Truncation of the intracytoplasmic tail results in a receptor that binds ligand but is unable to signal. The truncated receptor acts in a dominant-negative (DN) manner and thus renders the cell unable to respond to TGF-ß irrespective of the multiple potential cellular sources of production.16-22 Selective overexpression of a TGF-ß RII-DN in the intestinal epithelium permits assessment of the functional role in the epithelial compartment without the confounding effects of altered function of other cell types, and also ensures intestinal epithelial resistance to TGF-ß despite both autocrine and multiple paracrine sources of TGF-ß that could act on the intestinal epithelial cell.
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Materials and Methods |
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Establishment of Stable Cell Lines Expressing TGF-ß RII DN
Stable cell lines were established by transfecting the TGF-ß RII-DN construct into a rat nontransformed intestinal epithelial cell line (IEC)-6 using Lipofectamine Plus (Life Technologies, Gibco BRL, Boston, MA) per the manufacturer’s directions. Clones were grown in G418 selection media and were screened with primers from 5' location in the c-myc tag and 3' in the TGF-ß RII-DN and Western blotting.
Assessment of Stable Cell Lines Expressing TGF-ß RII DN
Cell Proliferation
Stable cell lines expressing either the TGF-ß RII-DN construct or empty vector were studied. To assess baseline cell proliferation, cells were plated at the same cell density on 100-mm plates in regular media (Dulbecco’s Modified Eagle Medium (DMEM) 10%, fetal calf serum, G418). The number of days to reach confluence was determined. These initial studies were correlated with thymidine incorporation assessed by seeding 12-well plates to 50% confluence followed by the addition of 0.4 or 40 pmol/L of TGF-ß for 24 hours. [3H]-Thymidine (NEN Life Science Products Inc., Boston, MA) was added to each well and after 4 hours the cells were rinsed in cold phosphate-buffered saline (PBS) two times, rinsed with trichloroacetic acid (TCA) (10%), and then 1 ml of 10% cold TCA was added to each well. TCA was removed and 1 ml of 0.1 mol/L NaOH was added to the well and incubated on ice for 10 minutes. After pipetting up and down, the suspension was added to vials containing 5 ml of scintillation fluid and counted. All experiments used a minimum of five plates/well/group and were repeated a minimum of three times.
Basal cell proliferation was also assessed by plating equal numbers of either the TGF-ß RII-DN or empty vector cell lines and determination of the duration of time it took to reach confluency. Eight 10-cm plates per group were used in each study and the study was repeated three times. All plates were assessed in a blinded manner every 12 hours from cell plating.
Migration and Wounding Assay
An intestinal epithelial cell wound-healing model was used.8
In brief, IEC-6 were plated and allowed to reach confluency in normal growth media. A wound was then made in the epithelial cell monolayer with a razor blade and the wound edge marked. Migration of cells from the wound edge at various times after wound induction and the distance of cell migration from the wound edge were measured using a standard cell culture microscope equipped with an ocular micrometer. Stable cell lines expressing the TGF-ß RII-DN under regulation by a CMV promoter, or an empty vector control were studied. Cells were plated at 
80% confluency and wounding performed after confluent. TGF-ß was added on wounding at a dose of 40 pmol/L and cell migration from the wound edge was determined on coded plates in a blinded manner at 24 hours. Each study consisted of a minimum of 12 plates per group and was repeated three times.
TGF-ß RII-DN-Expressing Transgenic Mice
The construct expressing TGF-ß RII-DN under the control of the LFABP-PTS4 promoter construct was used in the transgenic studies. The construct was prepared by cloning the LFABP into the Spe and HindIII sites and downstream of a c-myc tag (EQKLISEEDL) the TGF-ß RII-DN was cloned, in-frame, into NcoI and NotI sites of the CDM 7 vector that contained a SV40 polyadenylation sequence. The linearized construct was purified by agarose gel electrophoresis followed by extraction using QIAquick gel extraction kits (Qiagen Inc., Santa Clarita, CA). After dialysis against injection buffer (5 mmol/L Tris, pH 7.4, 5 mmol/L NaCl, and 0.1 mmol/L ethylenediaminetetraacetic acid, pH 8.0), the DNA was used for pronuclear injection of 129/SvJ mouse oocytes that were transferred to pseudopregnant mice using standard techniques. Mice were screened by a PCR-based approach as well as by Southern blotting. Three lines of TGF-ß RII-DN mice were established on a DBA background and negative littermates bred as controls.
Fertility, growth, and survival were determined for all lines of animals. Mice were sacrificed at 2, 4, 12, 24, and 52 weeks of age. All organs were examined including the intestine, liver, and kidneys. Tissue was processed for histology in a routine manner after hematoxylin and eosin (H&E) staining and examined by light microscopy using coded slides in a blinded manner.
Characterization of the TGF-ß RII-DN Transgenic Mice
Myeloperoxidase (MPO) Assay
MPO was determined to assess colonic granulocyte infiltration in all animals sacrificed at the above time points. Tissue samples from the proximal and distal colon were removed, rinsed in saline, and then immediately snap-frozen on dry ice and processed as described previously.22 An enzyme-linked immunosorbent assay plate reader was used to assess absorbance and was set at 460 nm and absorbance determined for three separate 30-second intervals. One unit of MPO activity was defined as 1 µmol of H2O2 broken down to H2O and O (resulting in a change in absorbance of 1.13 x 102).
Aberrant Crypt Foci and Cell Proliferation
Wild-type and TGF-ß RII-DN mice were sacrificed at 3 and 12 months of age for assessment of crypt foci and epithelial cell proliferation (a minimum of five animals per group). For assessment of aberrant crypt foci the entire small bowel and colon was removed and rinsed with ice-cold saline. The bowel was fixed by filling it with 10% phosphate-buffered formalin (pH 7.4) and ligating both ends. After 1 hour the bowel was incised and fixed for a further 24 hours after by staining with 0.2% methylene blue in 0.9% saline. The number of aberrant crypt foci in the small intestine and colon was determined by an observer blinded to the animal group and age using a dissecting microscope at x40 magnification as previously described.23
Cell proliferation was determined by bromo-2-deoxyuridine (BrdU) (Sigma Chemical Co., St. Louis, MO) staining. Mice, 3 and 12 months of age, were assessed with a minimum of five animals per group (wild type versus TGF-ß RII-DN). BrdU (50 mg/kg) was administered intraperitoneally 1 hour before sacrifice and tissue was processed and stained with anti-BrdU antibodies as described previously.24 One unit in a BrdU-labeling index was defined as the number of positive staining cells x 100/total number of cells.24 A total of 10 complete colonic crypts were read per animal, 5 from the proximal colon and 5 from the distal colon. Similarly, five crypts from the proximal small intestine (jejunum) and five from the distal small intestine (ileum) were assessed for the small intestine.
Western Blotting for c-myc-Tagged TGF-ß RII-DN
The colon and small bowel were removed, rinsed in ice-cold 1x PBS, and suspended in ice-cold lysis buffer (600 µl/100 mg tissue) containing 1x PBS, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 0.7 mmol/L ethylenediaminetetraacetic acid, and Roche Molecular Biochemicals complete Mini protease inhibitor cocktail tablets (Roche Molecular Biochemicals, Indianapolis, IN). Colonic and small intestinal mucosal isolates were prepared using the edge of a clean microscope slide to scrape the epithelium to submucosa from the muscularis in the presence of lysis buffer. These isolates were homogenized for 15 seconds using a Polytron tissue homogenizer (Brinkmann Instruments Inc., Westbury, NY), and centrifuged at 16,000 x g for 20 minutes at 4°C. The samples were heated in loading buffer for 2 minutes at 85°C, and loaded into a 10 to 20% Tricine gel (Novex, San Diego, CA) and transferred onto polyvinylidene difluoride membrane (Millipore Corp., Bedford, MA). Blots were blocked for 1 hour at 23°C in a blocking solution containing 5% dry milk, 0.1% bovine serum albumin, and 0.05% Tween-20 in 1x PBS, and then incubated in mouse monoclonal anti-c-myc antibody 10 µg/ml (clone 9E10, Ab-1; Calbiochem, Cambridge, MA) in the above blocking solution. Blots were then washed in 1x PBS and 0.05% Tween-20 (three washes of 20 minutes each) and incubated with horseradish peroxidase-linked goat anti-mouse antibody (Amersham Life Sciences, Arlington Heights, IL) at 1:10,000 in the same blocking solution for 60 minutes at 23°C. Antibody detection was performed using Renaissance chemiluminescent reagents (NEN Life Science Products Inc., Boston, MA), according to the manufacturer’s instructions.
Immunohistochemical Localization of TGF-ß RII-DN
Serial 6- to 10-µm frozen sections were rehydrated and treated for 10 minutes at 90°C in TUF reagent (Cedarlane Laboratories Ltd.). The sections were rinsed in PBS, blocked for 2 hours at room temperature with 15% normal goat serum in PBST (PBS containing 0.1% Triton X-100), washed in PBST, and incubated with 1:500 anti c-myc antibody (clone 9E10, Ab-1; Calbiochem) in 15% normal goat serum/PBST, overnight at 4°C. Sections were washed and incubated with the secondary antibody (goat anti-mouse IgG conjugated to CY3; Jackson ImmunoResearch Laboratories, West Grove, PA). After washing, slides were mounted in Fluorsave mounting media (Calbiochem). Anti-nuclear staining was done with a DNA-specific dye Hoechst 33258 (Sigma Chemical Co.) as per the manufacturer’s guidelines.
Induction and Assessment of Colitis
All mice were of a DBA background and matched by age, sex, and body weight. Animals were ear-tagged in a blinded manner so investigators assessing mice daily were also blinded to group. Animal experiments were performed in accordance with National Institutes of Health guidelines and protocols approved by the Subcommittee on Research Animal Care at the Massachusetts General Hospital and Harvard Medical School.
Colitis was induced by addition of dextran sodium sulfate (DSS) (molecular weight, 40,000, lot no. 3073B; ICN Biomedical, Aurora, OH) to drinking water (2.5% or 7.5% w/v in distilled water) as described previously.25 Animals were assessed daily and mean DDS/water consumption and body weights were recorded. The severity of diarrhea was assessed daily using a 0 to 3 scale; 0 = normal, 1 = soft, 2 = very soft but formed, 3 = liquid stool. Fecal blood was assessed by resuspending a fecal pellet in 400 µl of H2O. After brief centrifugation 40 µl of supernatant was added to a 0.5 x 0.5-cm piece of SENSA paper (SmithKline Diagnostics Inc., San Jose, CA), allowed to air-dry and developed with one drop of SENSA developer solution. The presence of blood results in a white to blue change that is proportional to the amount of blood presence in the sample. The intensity of the SENSA color change was scored by observers blinded to animal group and treatment on a 0 to 4 scale; 0 = nil, 1 = faintly blue, 2 = moderately blue, 3 = dark blue, and fecal blood visible to the eye was scored as a 4. The reproducibility of both the diarrhea and fecal blood scoring systems has been reported previously.25 Adhesion score was defined as: 0 = no adhesions to the colon, 1 = one area of adhesion to the colon, 2 = two areas of adhesion to the colon, 3 = three or more areas of adhesion to the colon. Macroscopic bowel thickening was assessed and measured and presented as the percentage of colon length thickened. All assessments were done in a blinded manner on coded animals.
On sacrifice the colon was removed, opened along the mesenteric border, and fecal material removed. Tissue was removed, fixed in 10% neutral buffered formalin, embedded in paraffin, sectioned, and stained with H&E in standard manner. MPO assay was also performed at various time points as an index of colonic inflammation as noted above.
To assess recovery after induction of colitis, DSS-colitis was induced in TGF-ß RII-DN and age- and sex-matched control mice by adding low-dose DSS (2.5% w/v) to their drinking water. Mice were followed as above for a period of 15 days at which time DSS was discontinued. Mice from both groups were assessed as above and the remainder of the animals in both groups was followed for a further 10 days after discontinuation of DSS exposure. Mice were then sacrificed and assessed as above.
Statistical Analysis
Data are presented as the mean ± SEM. Parametric data were analyzed using a one-way analysis of variance followed by a Dunnett multiple comparisons posttest. Nonparametric data (scoring) were analyzed using a Kruskal-Wallis test (nonparametric analysis of variance) followed by a Dunn’s multiple comparisons posttest. An associated probability (P value) of <0.05 was considered significant. Survival curves were created using the Kaplan-Meier method and survival comparisons were performed using the log-rank or Mantel-Haenszel test that generate a two-tailed P value. All statistical analysis was performed with Graph Pad Instat and Prism 3.0 programs (San Diego, CA).
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Results |
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. Cell lines expressing TGF-ß RII-DN were markedly resistant to TGF-ß-induced inhibition of cell proliferation at both 4 and 40 pmol of TGF-ß (Figure 1b)
confirming the functional effect of the construct expression in blocking response to TGF-ß. Cell lines expressing TGF-ß RII-DN also had more rapid basal proliferation as noted by a shorter time to grow to a confluent monolayer after a 5:1 split (an equal number of cells plated per group) with the TGF-ß RII-DN cells reaching confluency by 3.50 ± 0.27 days and control cells by 5.13 ± 0.23 days (eight 10-cm plates assessed per group, P = 0.0004). These effects are consistent with concurrent blockade of endogenous TGF-ß known to be produced by these cells.
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Wounds were established in cultured intestinal epithelial cell monolayers to determine and reconfirm the importance of TGF-ß in repair processes in vitro suggested by earlier studies. After wound induction, the baseline migration on average was 45% slower in the stable cell lines expressing the TGF-ß RII-DN compared to cells with empty vector (mean migration at 24 hours, 9.49 ± 2.19 mm versus 17.20 ± 2.36 mm, respectively; P < 0.001), as shown in Figure 2
. When TGF-ß-induced wound healing was assessed, 40 pmol/L of TGF-ß failed to enhance wound healing in the cell lines with TGF-ß RII-DN, whereas there was a marked increased rate of migration in the empty vector control cell lines (P < 0.001) [data presented as TGF-ß-induced change in migration as a percentage of baseline (no TGF-ß) migration; Figure 2, a and b
].
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After confirmation of effectiveness of the TGF-ß RII-DN as a tool to blockade TGF-ß response by intestinal epithelial cells in vitro, transgenic mice expressing TGF-ß RII-DN restricted to intestinal epithelial cells via the PTS4 modification of the LFABP promoter (LFABP-PTS4) were established. Expression of the TGF-ß RII-DN was assessed by reverse transcriptase-PCR after DNase treatment and was found to be restricted to the colon, ileum, and jejunum as anticipated (Figure 3)
. No significant expression was detected in the stomach, duodenum, heart, kidney, liver, and skeletal muscle. Colonic expression of the encoded protein was confirmed by Western blot analysis and immunohistochemistry (Figures 4 and 5, a and b)
. Immunohistochemistry demonstrated greater expression in epithelial cells that were closer to the luminal surface compared to epithelial cells located near the crypts (Figure 5, a and b)
. Two of the three lines established had variable levels of expression of the RII-DN via Western blotting, Northern blotting, and reverse transcriptase-PCR. Immunohistochemical localization studies showed patchy expression of the c-myc-tagged RII-DN in these two lines, thus these lines likely represented mosaics that have been commonly described using this promoter.26,27
Thus, for all subsequent studies only the line with uniform intestinal epithelial cell expression of the RII-DN was used.
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). Furthermore, no tumors or aberrant crypt foci were noted in any of the animals examined up to 52 weeks of age and there were no differences in intestinal epithelial cell proliferation assessed by BrdU labeling at 12- and 52-week time points (Figure 6)
. The lack of alteration in cell proliferation is somewhat surprising given that TGF-ß is thought to act as a brake in cell proliferation. The finding that cell proliferation was seemingly unaffected suggests that in vivo other factors play either a dominant or compensatory role. In addition, no differences in epithelial apoptosis in the small and large intestine as assessed by Apoptag staining were present in the TGF-ß RII-DN compared to wild-type mice.
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Colitis was induced by administration of DSS in drinking water. Transgenic TGF-ß RII-DN mice and littermate control animals consumed the same amount of DSS. The TGF-ß RII-DN mice were more susceptible to DSS-induced colitis as shown by a greater reduction in basal body weight and more fecal blood loss (Figure 7, a and b)
. Although diarrhea scores were equivalent, histological assessment of the surviving animals revealed markedly more severe colitis in the TGF-ß RII-DN mice than littermate control mice (Figure 8, a and b)
. The increased severity of DSS-induced colitis resulted in a marked reduction in survival of the mice expressing the TGF-ß RII-DN given the same amount of DSS (Figure 9)
.
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After 15 days, DSS was removed from the drinking water. During this recovery phase the wild-type animals rapidly improved, returning to their basal body weights, by day 20. In contrast, the basal body weights of TGF-ß RII-DN failed to return to baseline even by day 25, 10 days after stopping the DSS (Figure 10a)
. By day 25 the TGF-ß RII-DN mice had significantly more fecal blood loss (P = 0.002), diarrhea (P = 0.045), and after sacrifice more adhesions (P = 0.003) compared to similarly treated wild-type animals (Figure 10b)
. Macroscopically the extent of thickening of colon was also markedly less in the wild-type animals with only 2.8% (±1.5%) colonic bowel wall appearing thickened versus 38% (±5.2%) in the TGF-ß RII-DN mice (P < 0.001). Comparable differences were also present on histological examination (Figure 10, c and d)
and correlated with greater levels of colonic MPO activity in the TGF-ß RII-DN mice (P < 0.001, Figure 10e
). The failure of the TGF-ß RII-DN mice to recover from the DSS exposure resulted in increased mortality in these animals compared to wild-type mice (Figure 10f)
.
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TGF-ß has been proposed to play a key role in regulating intestinal epithelial cell migration and wound repair. Previous studies using an in vitro wounding assay demonstrated that stimulation of IEC-6 migration by interleukin-1ß, interferon-
, epidermal growth factor, and TGF-
is TGF-ß-dependent.7,8
Consistent with these findings in the present study stable cell lines expressing TGF-ß RII-DN had impaired wound repair in the same in vitro wounding assay. Because epithelial cell migration from the lesion edge resulting in re-epithelialization, is one of the initial responses after gastrointestinal injury, disrupting the normal epithelial cell migration conceptually could result in increased susceptibility to injury. This inference was confirmed in the transgenic TGF-ß RII-DN mice in vivo. Continued exposure to DSS results in epithelial injury and severe disease transmural inflammation. Histological assessment at varying time points during DSS exposure showed both injury and repair with clear re-epithelialization of denuded ulcerated areas. Exposure to high enough concentrations of DSS results in injury that outweighs repair and produces severe extensive ulceration and inflammation.
Intestinal wound repair was indirectly assessed by recovery experiments. In these studies, continued exposure to 2.5% DSS resulted in injury that appeared to be similar in severity in the TGF-ß RII-DN and wild-type control mice. When the concentration of DSS was increased (to 7.5%) the TGF-ß RII-DN mice clearly were more susceptible to DSS-induced injury. In the recovery study, wild-type animals rapidly recovered from the injury with almost normal appearing mucosa 10 days after cessation of DSS. Conversely, many of the TGF-ß RII-DN animals failed to recover and the disease process continued leading to death. Histological assessment showed continued active inflammation in these mice and numerous ulcerated areas failed to become completely re-epithelialized. These in vivo phenotypic characteristics closely mirrored the effects seen in vitro. Collectively these findings suggest that despite the multiplicity of regulatory peptides found to promote restitution in vitro, TGF-ß is absolutely required.
The important role of TGF-ß signaling in the pathogenesis of inflammatory bowel disease has been further delineated by the studies of Monteleone and colleagues,31 which showed that patients with inflammatory bowel disease have unregulated SMAD-7 expression. SMAD-7 inhibits TGF-ß signaling by preventing phosphorylation of SMAD 2/3 which in turn prevents the formation of the SMAD 2/3/4 complex and target gene induction.31,32 An anti-sense approach was used to block SMAD7, which restored TGF-ß signaling in mononuclear cells isolated from patients with Crohn’s disease.31 Thus it appears that TGF-ß plays a key role in regulating intestinal inflammation and altering normal TGF-ß signaling either by targeted disruption of TGF-ß, altering the members of the SMAD family of proteins involved in signal transduction or by disrupting signaling via a DN-RII approach, as was done in this study, results in more severe intestinal inflammation.
The role of TGF-ß in tumorigenesis has been well documented. The majority of tumors associated with hereditary nonpolyposis colorectal cancer syndrome and ulcerative colitis-associated colonic adenocarcinoma have mutations resulting in down-regulation of TGF-ß responsiveness.10-12 Remarkably, the specific defects in these tumors in humans result in a similar condition that was induced in the TGF-ß RII-DN mice with a mutation resulting in the loss of downstream signaling domain of the TGF-ß receptor RII.11,12 The exact mechanism by which such TGF-ß RII mutations promote tumorigenesis are unclear but TGF-ß RII in concert with TGF-ß RI normally induce signal transduction to the nucleus via members of the SMAD family of proteins. The importance of TGF-ß in tumor suppression was highlighted by a study by Mikhailowski and colleagues33 that showed that exogenous TGF-ß1 marked reduced colonic tumor formation in a rat model of colon cancer and by the study of MacKay and colleagues that showed transfection of native TGF-ß RII to colon cancer lines resulted in inhibition of cell growth and a reduction in the malignant phenotype of the transfected cells.34 In a study by Zhu and colleagues35 SMAD-3-deficient mice frequently developed metastatic colonic adenocarcinoma at an early age, however, a study by Yang and colleagues36 found that only 1 of 30 mice followed for 6 months developed colon cancer but the mice frequently has intestinal inflammation. The reason for the discrepancy between these studies on the disruption of SMAD-3 is unclear but the targeting vectors were different, as were the murine strains.
Notwithstanding these previous observations in colonic tumors, TGF-ß does not seem to be essential for the normal development and regulation of intestinal homeostasis because the down-regulation of TGF-ß responsiveness in the present study did not result in abnormal intestinal development or baseline intestinal inflammation in a nonsterile environment. No differences in intestinal epithelial cell proliferation or apoptosis could be detected in the TGF-ß RII-DN mice suggesting that, at baseline conditions, TGF-ß may not be the key regulator of these epithelial cell responses. Furthermore, down-regulation of epithelial cell TGF-ß responsiveness did not lead to an increase in dysplasia or neoplasia again suggesting that intestinal epithelial cell proliferation and differentiation are not solely regulated by TGF-ß. Alternatively, some residual response may be present in the intestinal epithelial cells of the TGF-ß RII-DN mice that was sufficient to regulate basal function but inadequate to respond to a significant insult such as DSS exposure. Another possible explanation for the lack of changes in cell proliferation, apoptosis, and tumorigenesis is that LFABP-PTS4 promoter-directed expression of the TGF-ß RII-DN appeared to be less in replicating cells at the base of the crypts but increased dramatically as cells moved up the crypts toward the luminal surface so that the down-regulation TGF-ß responsiveness was more marked in the nonreplicating cells closer to the luminal surface.
The role of intestinal goblet cell TGF-ß responsiveness was recently assessed by Hahm and colleagues37 using a different DN TGF-ß RII approach. In their study the expression of the TGF-ß RII-DN was driven by the intestinal trefoil promoter, which should limit expression to intestinal goblet cells. In a nongerm-free environment the intestinal trefoil-TGF-ß RII-DN developed spontaneous colitis whereas in a germ-free environment the animals were free of colitis but more susceptible to DSS-induced intestinal injury.37 The role of TGF-ß in regulating goblet cell function has not been previously assessed and thus the mechanism by which such a goblet cell-specific construct results in intestinal injury in a nongerm-free setting and increased susceptibility to injury is unclear and was not provided in the study. However, this is consistent with earlier studies from this laboratory demonstrating a role for goblet cells distinct from columnar epithelial cells in determining susceptibility or resistance to colitis.38 The role of TGF-ß has been assessed in intestinal epithelial cells in vitro and our study presented here clearly shows that overexpression of TGF-ß RII-DN in intestinal epithelial cells affects wound healing in vitro and down-regulation of epithelial cell TGF-ß responsiveness results in increased susceptibility to intestinal injury and delayed wound healing in vivo.
In summary, the present studies have allowed the delineation of the functional importance of TGF-ß within the epithelial cell compartment without the confounding effects of TGF-ß in lamina propria immune cells and other cell populations.
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Supported by National Institutes of Health grants DK43352, DK41557, and DK53304 as well as a grant from Alberta Heritage Foundation for Medical Research.
Accepted for publication November 6, 2002.
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