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From the Roy and Ann Foss Interventional Cardiology Research Program, * Terrence Donnelly Heart Centre, St. Michael’s Hospital and University Health Network, University of Toronto, Toronto, Ontario, Canada; the Cardiovascular Gene Therapy Program, Yale University, New Haven, Connecticut; and the Experimental Cardiology Laboratory, University Medical Center, Utrecht, The Netherlands
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Abstract |
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50% at 10 weeks compared to ß-galactosidase-transfected or balloon-injured, non-transfected controls. This study shows that decorin binds to PDGF and inhibits its stimulatory activity on SMCs by preventing PDGFR phosphorylation. Decorin overexpression reduces intimal hyperplasia and collagen content after arterial injury. Decorin may be an effective therapy for the prevention of intimal hyperplasia after balloon angioplasty.
Decorin is a small proteoglycan that consists of a single glycosaminoglycan side chain linked to a core protein containing leucine-rich repeats of about 24 amino acids.8,9 It is found in the ECM of a variety of tissues and cell types.8,9 Decorin interacts with a variety of proteins that are involved in matrix assembly,10 and in the regulation of fundamental biological functions such as cell attachment,11 migration,12 and proliferation.13,14 Decorin inhibits cell proliferation in several cell types, including cancer cells.13 It mediates its regulatory effects either directly by up-regulating cyclin-dependent kinase inhibitors such as p21 and p27, or through its ability to interact with growth factors.15,16 It is well established that decorin binds to TGF-ß17 and inhibits its biological activity in a number of cell types, including rat aortic SMCs.16 However, it is not known whether decorin may also exert its regulatory effects by interacting with and modulating the activity of PDGF, another prominent growth factor involved in arterial repair. Therefore, the objectives of this study were to assess decorin-PDGF and decorin-PDGF receptor (PDGFR) interactions, the effects of decorin on PDGFR phosphorylation and PDGF-stimulated SMC functions in vitro, and effects of decorin overexpression on arterial repair in a rabbit carotid balloon-injury model that is characterized by PDGF up-regulation.6,7
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Materials and Methods |
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Solid-solid-phase binding and affinity cross-linking assays were performed to assess PDGF and decorin interactions.
Solid-Phase Radioligand-Binding of 125I-PDGF to Decorin
Bovine articular cartilage decorin (Sigma, St. Louis, MO) was coated (1 µg/well) onto the surface of a 96-well plate (Nalge Nunc International, Denmark).17 Nonspecific binding was blocked with 1% bovine serum albumin (BSA) in binding buffer for 3 hours at 37°C. Immobilized decorin was then allowed to bind to 4 x 104 cpm of 125I-PDGF-BB (Amersham Biosciences Corp., Piscataway, NJ) in the presence or absence of increasing concentrations of chondroitin sulfate (Sigma), BSA, or unlabeled PDGF-BB (R & D Systems Inc., Minneapolis, MN) as competitors. After a 6-hour incubation at 37°C, bound 125I-PDGF was solubilized in 0.3 mol/L NaOH, 1% Triton X-100 for 30 minutes and the radioactivity of each well was measured using a gamma counter (WALLAC, 1470 WIZARD). Nonspecific binding was determined by adding 100-fold excess unlabeled PDGF to the incubation mixture.
Affinity Cross-Linking of Decorin with PDGF and Immunoprecipitation Using Decorin Antibody
Decorin (1 µg) was mixed with PDGF-BB (0.01, 0.1, or 1.0 µg) in 200 µl of binding buffer18 and incubated at 37°C for 3 hours. The proteins were then cross-linked with 0.3 mmol/L disuccinimidyl suberate (DSS; Pierce, Rockford, IL, USA) at 37°C for 30 minutes and the reaction was terminated by adding TBS buffer (10 mmol/L Tris-base buffer, pH 8.0, 150 mmol/L NaCl). Cross-linked proteins were immunoprecipitated with anti-decorin monoclonal antibody 6D619 (generous gift from Dr. Paul G. Scott, University of Alberta, Edmonton, Alberta) using protein G sepharose (Amersham Biosciences Corp) at room temperature for 1 hour. The suspension was centrifuged and beads were washed four times with ice-cold TBS buffer. To recover specifically bound proteins, beads were suspended in 50 µl of 1X SDS-PAGE sample-loading buffer, mixed for 30 minutes at room temperature, and centrifuged. Proteins in 25 µl of supernatant were analyzed by 4 to 20% SDS and electrotransferred onto a nitrocellulose membrane (Bio-Rad, Hercules, CA, USA). Decorin-PDGF complex was detected with anti-decorin and anti-PDGF antibodies as described below.
Immunoblotting of Decorin-PDGF Complex Using Decorin and PDGF Antibodies
The membrane was immunoblotted with anti-decorin monoclonal antibody 6D6.19 Anti-mouse IgG-HRP (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) was used for detection of primary antibody. The membrane was stripped and re-immunoblotted with goat anti-PDGF antibody (R & D Systems). Anti-goat IgG-HRP (Santa Cruz) was used for detection of primary antibody. Detection was performed using a chemiluminescence peroxidase detection system (Sigma) and exposure to BioMax film (Kodak).
In Vitro Studies
Cell Culture
Rabbit aortic SMC from passages 5 and 6 were cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 2% penicillin-streptomycin and 10% fetal calf serum. For DNA synthesis and cell proliferation assays, cells were initially seeded at 5 x 104 per well into 24-well plates and at 104 per well into 96-well plates, respectively. For all other experiments, cells were grown to confluency. In each assay, cells were serum-starved for 48 hours and then pretreated for 30 minutes with the indicated concentration of decorin followed by treatment with PDGF-BB (10 ng/ml) for 24 hours.
PDGFR Expression
Confluent monolayers of cells were incubated in serum-free medium containing PDGF-BB in the presence or absence of decorin. After 24 hours of incubation period, cells were washed with PBS and lysed in 1X SDS-PAGE sample-loading buffer. Samples containing 50 µg of proteins were separated by 4 to 20% SDS-PAGE and electrotransferred onto a nitrocellulose membrane. Membranes were immunoblotted with goat anti-human PDGFR-
or -ß antibodies (R&D Systems). Anti-goat IgG-HRP (Santa Cruz) was used for detection of primary antibody and revealed using chemiluminescence detection system followed by autoradiography.
PDGFR ß Phosphorylation
Confluent monolayers of cells were incubated in serum-free medium containing PDGF-BB in the presence or absence of decorin for 1 hour at 4°C. Cells were extracted as previously described.20 Lysates were incubated at 4°C for 6 hours with 2 µg of goat anti-human PDGFR-ß antibody and then 30 µl protein of G Sepharose (Pharmacia Biotech) was added and mixed at 4°C overnight. Immunoprecipitated receptors were released from protein G pellets by boiling for 5 minutes in 1X SDS-PAGE sample-loading buffer containing 2-mercaptoethanol and separated by 8% SDS-PAGE. Proteins were electrotransferred onto a nitrocellulose membrane and immunoblotted with anti-phosphotyrosine 4G10 monoclonal antibody (Upstate Biotechnology, Lake Placid, NY). Goat anti-mouse secondary antibody was used for detection of primary antibody. The membrane was stripped and re-immunoblotted with goat anti-human PDGFR-ß antibody. Detection was performed using chemiluminescence detection system followed by autoradiography.
Binding of 125I-PDGF to Cells or PDGF Receptors
Cell binding assay and affinity cross-linking were performed as described previously.18 Briefly, monolayers of confluent cells were washed three times with cold binding buffer and incubated in binding buffer for 30 minutes at 4°C. Confluent cells were pretreated for 30 minutes with decorin (30 µg/ml) in binding buffer, followed by adding 125I-PDGF (0.1 nmol/L). 125I-PDGF was also added to two other groups that were not pretreated with decorin. One group was given unlabeled PDGF (10 nmol/L) as a competitor to show specificity of the binding while the other group (control group) did not receive unlabeled PDGF. All groups were incubated at 4°C for 4 hours. Cells were then washed four times with binding buffer. For binding assay, cells were solubilized with 1X SDS-PAGE sample- loading buffer. The radioactivity of aliquots of soluble fractions was measured using a gamma counter. For affinity cross-linking assay, cells were cross-linked with DSS (0.3 mmol/L) at 4°C for 15 minutes and lysed in 1X SDS-PAGE sample-loading buffer. Soluble fractions containing 50 µg protein were analyzed by 4 to 12% SDS-PAGE. The gel was dried followed by autoradiography using BioMax film.
Cell Necrosis and Apoptosis
Cells were incubated in serum-free medium containing 30 µg/ml of decorin for 24 hours. For cell necrosis, lactate dehydrogenase (LDH) was measured in the medium using CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Promega Corporation, Madison, WI) according to the manufacturer’s instructions.
For cell apoptosis, cells were washed with PBS, lysed in 1X SDS-PAGE sample-loading buffer and centrifuged. Supernatants containing 50 µg protein were analyzed by 4 to 20% SDS-PAGE and electroblotted onto a nitrocellulose membrane. The membrane was immunoblotted with rabbit polyclonal anti-caspase-3 (CPP32) Ab-4 (NeoMarker, Fremont, CA). Anti-rabbit IgG-HRP (Santa Cruz) was used for detection of primary antibody. The membrane was then stripped and re-immunoblotted with anti-mouse monoclonal anti-ß actin antibody (Sigma). Goat anti-mouse secondary antibody (Sigma) was used. Detection was performed using chemiluminescence detection system (Sigma) followed by autoradiography.
Growth Assays
For DNA synthesis, 1.0 µCi/ml of 3H-thymidine (Amersham) was added to the cultures 24 hours after treating cells with PDGF-BB in the presence or absence of decorin and incubated at 37°C for 6 hours. DNA synthesis was measured as previously described.16 For cell proliferation, cells were incubated with PDGF-BB (10 ng/ml) in the presence and absence of decorin (20 µg/ml) for the indicated time periods and cell density was determined as described by Hou et al21 Briefly, cells were fixed with 4% paraformaldehyde and stained with 0.5% toluidine blue in 4% paraformaldehyde for 5 minutes. Cells were rinsed in water twice and solubilized with 100 µl of 1% SDS. Absorbance was measured in a microtiter plate reader (Molecular Devices) at 595 nm.
Migration Assay
Neuro Probe 48-well microchemotaxis chambers (Costar, Corning Inc., Corning, NY) with PVP-free polycarbonate filter (8.0 µm pore size) were used. The bottom well of the Boyden chamber was filled with 250 µl of serum-free medium containing PDGF-BB (10 ng/ml). Quiescent cells were trypsinized and resuspended in serum-free medium with or without decorin and incubated for 30 minutes at 37°C. Cells (2 x 104) were then added to the upper well of the microchemotaxis chamber and incubated for 5 hours at 37°C. Cells that migrated to the lower side of the filters were fixed and stained with the Diff-Quick staining kit (VWR Laboratory). The filters were mounted on glass slides and counted by light microscopy using x100 magnification. The results were expressed as number of migrated cells per high power field (HPF).
Collagen Synthesis Assay
Ascorbic acid (50 µg/ml) and 14C-proline (5 µCi/ml) were added to the cultures 24 hours after treating cells with PDGF-BB in the presence or absence of decorin and incubated at 37°C for 6 hours. Collagen synthesis was measured in the culture medium using a bacterial collagenase digestion method.22
In Vivo Effects of Decorin Overexpression in Balloon-Injured Arteries
Carotid Artery Model
The animal experiments were performed in accordance with guidelines set out by the University of Toronto and approved by the St. Michael’s Hospital Animal Care Committee. A double-injury carotid artery model was done in 48 normolipemic male New Zealand White rabbits weighing 3.5 to 4.0 kg as previously described.23 Both carotid arteries were injured with a 3.5-mm diameter balloon angioplasty catheter. At 3 weeks, a second balloon injury was performed. Immediately after second injury, the arteries were surgically exposed and the balloon-injured segments were temporarily isolated with ligatures. Gene transfer to these arterial segments was achieved using recombinant adenovirus vectors prepared by homologous recombination as previously described.24 Briefly, a 1.5-kb rabbit decorin cDNA was cloned into the shuttle vector pCMVPLPA in which expression of the cDNA is driven by the CMV promoter. Recombination between this shuttle vector and the adenovirus 5 genomic vector pJM17 produced recombinant adenovirus encoding the decorin expression cassette. A control ß-galactosidase encoding adenovirus was prepared similarly. In vivo gene transfer was achieved by injecting 109 pfu of adenovirus in a 100-µl volume into the vessel lumen to distend the balloon-injured arterial segment. Ligatures were positioned on either side of a 20-mm segment to prevent drainage and the volume was allowed to dwell for 20 minutes before repairing the arterial puncture and restoring perfusion. The contralateral artery was treated in a similar fashion. A third group underwent the two serial balloon injuries without gene transfection to serve as balloon-injured, non-transfected controls. The animals were sacrificed at three time points: 24 hours for transgene detection, 1 week for decorin immunostaining and cell proliferation, and 10 weeks for measurement of collagen content, intimal hyperplasia, and immunohistochemistry. At sacrifice, treated arteries were divided into two (15 mm and 5 mm) segments. The 15-mm segments were used for collagen measurement and the 5-mm segments were fixed in 10% buffered formalin. Arteries removed at 24 hours were snap-frozen in liquid nitrogen and stored at -80°C until use.
Decorin Transgene Detection by PCR
DNA was extracted from rabbit carotid arteries using DNeasy Tissue kit (Qiagen Inc.). The polymerase chain reaction (PCR) mixture contained 35 pmol each of sense primer (GTA CGG TGG GAG GTC TAT AT) from the CMV promoter and antisense primer (CCA CTG AAC ACA GAT T) from the rabbit decorin gene. The inclusion of the CMV promoter sequence in the sense primer ensured specificity for identifying exogenous decorin cDNA. PCR was started by adding Master Mix (TaqPCR Master Mix kit, Qiagen, Inc) and carried out for 42 cycles with a denaturation step of 60 seconds at 94°C, an annealing step of 60 seconds at 57°C, and extension step of 105 seconds at 72°C. An aliquot of each sample was separated by 2.0% agarose gel electrophoresis and visualized by ethidium bromide staining.
Cell Proliferation
Proliferating cells in arteries at 1 week following balloon injury were identified by immunohistochemistry using anti-Mib-1 antibody25 (1:500 dilution, DAKO Diagnostics). Mib-1-positive cells were counted in the intima, media, and adventitia of injured arteries using an image analysis system (Scion Image, Scion Corp) under x20 magnification. Proliferation rates were expressed as total Mib-1-positive cells/arterial cross-section.
Assessment of Inflammation
Arterial cross-sections from both 1-week and 10-week animals were stained for the presence of neutrophils and macrophages. Mouse monoclonal antibodies against rabbit neutrophils (1:50 dilution, Serotec Inc., NC) and rabbit macrophages, RAM11 (1:50 dilution, Dako, CA) were used on paraffin-embedded sections and examined under light microscopy. The total number of neutrophils and macrophages were counted at x40 magnification in two to three representative sections of each artery. The sections with the maximum number of neutrophils or macrophages were used for analysis.
Immunostaining for Decorin
Cross-sections from 1-week post-angioplasty arteries were immunostained with the anti-decorin antibody, at a dilution of 1:100.
Immunostaining for Fibronectin
Identification of fibronectin in 10-week post-angioplasty arteries was performed with a mouse monoclonal anti-human cellular fibronectin antibody (Chemicon International, Temecula, CA, 1:50 dilution). Paraffin-embedded, formalin-fixed arterial cross-sections were treated by microwave heating for 10 minutes in citrate buffer and endogenous peroxidase was blocked with 0.6% hydrogen peroxide for 45 minutes. Large bowel was used as a positive control. Negative controls for the arterial sections were done with omission of the primary antibody. All slides were reviewed by one of the authors (J.B.) without knowledge of the treatment group. Each layer of the vessel wall was semi-quantitatively scored using a 0 (no staining) to 4 (intense staining) scale.
Morphometric Analysis
Serial cross-sections (four to five per vessel) were obtained from the treated arteries and stained with Movat-pentachrome stain. Computerized morphometry (Scion Image, Scion Corp) was performed to determine intimal cross-sectional area (CSA) and intimal thickness.
Collagen Content and Density Measurement
Collagen content was determined biochemically using a hydroxyproline assay as previously described.2 Quantification of collagen density was performed in intima, media, and adventitia using picro sirius red staining and digital image microscopy with circularly polarized light as described by Sierevogel et al26 In short, a picro sirius red image was converted into a gray value image. Regions of interest (ROI) were drawn to select the different arterial layers. The total amount of gray values in each layer was determined for collagen content. Collagen density, expressed as gray-values (arbitrary unit)/µm2, was calculated by dividing the collagen content in each layer by the cross-sectional area of this layer.
Statistical Analysis
All measurements are expressed as mean ± SD. Student’s t-test was used for comparison between two groups while analysis of variance (ANOVA) was performed for multiple comparisons. All statistical analyses were performed using SPSS software (SPSS Inc., Chicago, IL). Statistical significance was defined as P < 0.05.
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In solid-phase binding assay, binding of 125I-PDGF to decorin was competed by chondroitin sulfate, BSA, and unlabeled PDGF (Figure 1A)
. Unlabeled PDGF competed with labeled PDGF in a concentration-dependent manner, yielding half-maximal inhibitory concentration of about 0.37 µg/ml and displaced more than 95% of labeled PDGF at 0.75 µg/ml. In contrast, chondroitin sulfate or BSA did not reach half-maximal inhibition and at 0.75 µg/ml they displaced 20% and 40% of labeled PDGF, respectively. The higher binding affinity of PDGF for decorin indicates specificity of decorin binding to PDGF.
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or decorin (Figure 1C)
antibody confirmed decorin-PDGF complex formation. In Vitro Studies
Effect of Decorin on PDGFR Expression
PDGFR- was not detected in SMCs. PDGF down-regulated PDGFR-ß expression in the presence or absence of decorin. Decorin did not affect PDGFR-ß expression (Figure 2A)
.
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Binding of 125I-PDGF to SMCs was similar in the presence (1081 ± 161 cpm/well) or absence (1015 ± 48 cpm/well) of decorin. Unlabeled PDGF that was used as a positive control competed with 125I-PDGF and reduced its binding (454 ± 64 cpm/well) to the cells. Similar results were obtained for cross-linking assay (Figure 2B)
where decorin did not prevent 125I-PDGF binding to receptors whereas unlabeled PDGF competed with labeled PDGF and reduced its binding to receptors. These results suggest that decorin does not prevent PDGF binding to cells or receptors.
PDGFR-ß Phosphorylation
PDGF stimulated PDGFR-ß phosphorylation which was almost completely inhibited by decorin (Figure 2C)
.
Effect of Decorin on Cell Necrosis and Apoptosis
LDH levels were similar in culture medium of decorin-treated cells and control non-treated or PDGF-treated cells (data not shown). There was also no evidence of active caspase-3 in decorin-treated, control non-treated or PDGF-treated cells in the cell apoptosis assay (Figure 3)
.
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Cells treated with PDGF showed a sixfold increase in DNA synthesis compared to control non-treated cells. Decorin inhibited PDGF-stimulated DNA synthesis in a dose-dependent manner (Figure 4A)
. To determine whether decorin had an effect on cell proliferation, the growth of SMCs were determined at 24 and 48 hours. Decorin significantly inhibited PDGF-stimulated cell proliferation at both time points (Figure 4B)
.
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An approximately twofold increase was observed in cell migration for the PDGF-treated cells compared to control non-treated cells. Decorin caused a dose-dependent inhibition of PDGF-stimulated cell migration (Figure 4C)
.
Effect of Decorin on Collagen Synthesis
A fourfold increase was observed in the rate of collagen synthesis for the PDGF-treated cells compared to control non-treated cells. Decorin caused a dose-dependent inhibition of PDGF-stimulated collagen synthesis (Figure 4D)
.
In Vivo Studies
Detection of Decorin Transgene
At 24 hours following injury and gene transfection, a 870-bp decorin cDNA fragment was identified in the decorin-transfected arteries but not detected in injured ß-gal-transfected, non-transfected injured or uninjured arteries (Figure 5A)
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At 1 week after balloon injury, decorin was detected in the media of decorin-transfected arteries (Figure 5B)
, but not in ß-gal-transfected, non-transfected injured or uninjured arteries.
Intimal Hyperplasia
The intimal CSA was significantly reduced by 50% in decorin-transfected arteries (Figures 6 and 7)
compared to ß-gal-transfected and non-transfected injured control arteries (P < 0.04). Similarly, the maximum intimal thickness was also reduced by 50% in decorin-transfected (0.09 ± 0.03 mm) compared to ß-gal-transfected (0.19 ± 0.15 mm) and non-transfected injured controls (0.17 ± 0.10 mm) (P < 0.04).
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The cell proliferation rates at 1 week post-angioplasty were not significantly different between the three groups (decorin-transfected arteries: 10 ± 8; ß-gal-transfected 14 ± 7; non-transfected injured: 9 ± 5 Mib-1-positive cells/arterial cross-section). In addition, the cellular proliferation rates were also similar for the three (intima, media, and adventitia) layers of the vessel wall between groups.
Inflammation and Cellular Infiltrate
Lack of active inflammation at both time points (1 week and 10 weeks) was evident by an absence of neutrophils or macrophages in the majority of treated arteries. In only one ß-gal-transfected artery at 10 weeks, six macrophages were present. Four neutrophils were detected in one decorin-transfected artery at 1 week.
Collagen Content and Density
At 10 weeks, there was a significant decrease in collagen content in decorin-transfected arteries compared to ß-gal-transfected and injured, non-transfected arteries, (228 ± 98 versus 343 ± 109 and 411 ± 331 µg hydroxyproline/arterial segment, respectively, P < 0.03). Based on the relationship between collagen content and intimal area, the decrease in collagen content in the decorin-treated arteries appeared to be proportional to the reduction in intimal area. This was supported by the collagen density data. There was no statistically significant difference in collagen density between the three groups in the adventitia (decorin: 101 ± 30 gray values/µm2; injured, non-transfected: 107 ± 23 gray values/µm2; ß-gal: 97 ± 21 gray values/µm2) or the intima (decorin: 5.9 ± 1.9 gray values/µm2; injured, non-transfected: 5.7 ± 4.2 gray values/µm2; ß-gal 7.7 ± 5.4 gray values/µm2).
Fibronectin Immunostaining
At 10 weeks, there was intense staining in the adventitia in all three groups with no or mild intimal staining in all three groups. There were no statistical differences in the intimal staining (decorin: 0.33±.55; injured, non-transfected 0.78±.83; ß-gal: 0.92±.80).
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Discussion |
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The inhibitory effect of decorin on PDGF-stimulated SMC functions was not due to cell apoptosis or necrosis. The novel mechanisms of decorin interactions with PDGF in our study were direct decorin binding to PDGF and acting as a potent inhibitor of PDGF receptor phosphorylation in response to PDGF. There was no effect on PDGF receptor-ß expression. The specificity of decorin binding to PDGF was demonstrated by a solid-phase binding assay in which unlabeled PDGF competed with 125I-PDGF for binding to decorin. This was also confirmed by affinity cross-linking using both PDGF and decorin antibodies which indicated PDGF binding to decorin in a concentration-dependent manner.
Despite important regulatory effects on ECM deposition, decorin expression is not up-regulated in human coronary restenosis specimens31 or after balloon injury in animal models,32 a finding confirmed in the present study in the ß-gal-transfected and the injured, non-transfected arteries. In decorin-transfected arteries, there was decorin expression evident at 1 week after transfection and a marked reduction in intimal cross-sectional area at 10 weeks. Since intimal area was reduced without a change in cell proliferation, reduced collagen content (50% compared to ß-gal-transfected and 80% compared to injured non-transfected arteries) appears to be a mechanism responsible for the inhibition of intimal thickening by decorin. This conclusion is also supported by in vitro results that showed complete inhibition of collagen synthesis in SMCs. An anti-fibrotic effect of decorin has been previously demonstrated in experimental lung33 and glomerulonephritis models.34 This is the first report of an anti-fibrotic effect of decorin overexpression in a vascular-injury model. In a rat carotid balloon-injury model, Fischer et al32 showed that cell-mediated decorin transfection decreased neointimal volume and increased intimal collagen density, based only on immunostaining. In contrast, our studies using a quantitative measurement of collagen content and collagen density showed decreased collagen content with no significant differences in collagen density in the intima or the adventitia. Moreover, we did not find any significant differences in fibronectin immunostaining in the intima between the three groups. This suggests that the intimal extracellular matrix is not qualitatively different between the decorin-transfected and the two control groups.
The mechanism by which decorin regulates ECM and exerts its anti-fibrotic effect is not clearly known. Previous studies have shown that decorin binds to TGF-ß17 and inhibits its activity in vitro16 and in vivo.33,35 Decorin-PDGF binding and inhibition of PDGFR phosphorylation by decorin reported in this study indicate an additional mechanism of ECM regulation by decorin. Decorin is also known to bind to collagen36-38 and inhibit collagen fibrillogenesis.39 These diverse effects of decorin make it an attractive therapeutic agent for several pathological arterial conditions characterized by intimal hyperplasia.
In conclusion, our study shows a novel interaction of decorin with PDGF, based on decorin-PDGF complex formation and inhibition of PDGF-stimulated PDGFR phosphorylation and SMC activities. Decorin overexpression significantly reduced both intimal hyperplasia and collagen content after balloon injury, demonstrating a potent beneficial anti-fibrotic effect for decorin.
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Footnotes |
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Supported by the Heart and Stroke Foundation of Ontario and dedicated to the memory of Robyn Strauss Albert.
N. N. and A. N. C. contributed equally to the manuscript.
Accepted for publication May 12, 2003.
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and transforming growth factor-ß1 during the development of accelerated graft vascular disease after clinical heart transplantation. Transpl Immunol 1999, 7:201-205[Medline]
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0.02 versus PDGF-treated cells.
X174): DNA standards. In the decorin-transfected arteries, a 870-bp fragment was identified by using primers for the CMV promotor sequence and the rabbit decorin gene. B: Decorin (brown staining) demonstrated in medial layer by immunohistochemistry at 1 week after angioplasty and transfection. Arrowhead, external elastic lamina; arrow, internal elastic lamina; l, lumen; m, media; a, adventitia.
