Mouse Models of Arteriosclerosis: From Arterial Injuries to Vascular Grafts
Am J Pathol Xu
165: 1
Supplemental Movie for Am J Pathol 2004; 165:1-10
Files in this Data Supplement:
Supplemental Movie
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Vein graft - cuff technique
(3-minute QuickTime movie, File Size: 13,411 KB)
Brief 3-minute movie which would be helpful for the investigators who wish
to use this model. When you watch the movie, you see that the right common carotid artery was mobilized free from the bifurcation at the distal end toward the proximal, cut in the middle, and a cuff placed at the end. The cuff was made of an autoclavable nylon tube 0.63 mm in diameter outside and 0.5 mm inside. The artery was turned inside out over the cuff and ligated. The vessel segment of either vena cava or jugular vein from donor was grafted between the two ends of the carotid artery by sleeving the ends of the vein over the artery-cuff and ligating them together with the 8-0 suture. As displayed in the movie, pulsation can be seen after removing the clamps. The complete grafting procedure required 30-40 min.
