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From the Department of Pathology and Molecular Medicine* and the Division of Nephrology, McMaster University, Hamilton; and the School of Optometry, University of Waterloo, Waterloo, Ontario, Canada
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Abstract |
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The lens is a relatively simple tissue composed of two cell types: epithelial cells and fiber cells. In the embryo, the lens consists of a highly proliferative monolayer of lens epithelial cells (LECs) that cover the anterior half of the lens.2 At the lens equator, these cells are stimulated to terminally differentiate into fiber cells by a gradient concentration of growth factors within the ocular media.3 In adults, lens proliferation and differentiation occurs near the lens equator, albeit at a slower rate than in the embryo. However, in a pathological situation such as occurs after ocular trauma, surgery, or systemic diseases like atopic dermatitis and retinitis pigmentosa, the anterior LECs can be triggered to proliferate and multilayer beneath the lens capsule.3,4 A proportion of these cells transform into plaques of large "spindle shaped" cells, or myofibroblasts, by a phenomenon known as epithelial-to-mesenchymal transition (EMT).5-7
The cytokine transforming growth factor-ß (TGFß) has been shown to play a role in lens disease and to induce these aberrant changes in LECs, including their conversion to myofibroblasts.3
The resultant myofibroblasts express contractile elements, like 
-smooth muscle actin (-SMA), and begin to secrete an abnormal accumulation of type I and III collagen.8
Additional extracellular matrix proteins are deposited, including tenascin and fibronectin, and as a result, fibrous anterior subcapsular cataract (ASC) plaques form that develop into distinct opacities in the lens. Similar to ASC, in secondary cataract, also known as posterior capsular opacification, LECs that remain within the capsule after cataract surgery are triggered to proliferate and migrate to the posterior lens capsule, where they frequently transform into myofibroblasts.9,10
Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that act as key regulators of tissue remodeling11
and have been shown to participate in a number of ocular diseases, including retinal disease, glaucoma, and corneal disorders.12
MMPs and tissue inhibitors of matrix metalloproteinases have also been examined in the normal and cataractous lens. Although some MMPs, such as MMP-9 and membrane-type 1-MMP, have been found to be constitutively expressed in the lens,13-15
other MMPs, such as MMP-2, are typically expressed after treatment with growth factors or during cataract formation. For example, Seomun et al16
reported that TGFß triggers MMP-2 mRNA expression in a human lens epithelial cell line and induces immunoreactivity for both MMP-2 and -SMA in the subcapsular plaques of rat lenses. TGFß has also been shown to stimulate secretion of MMP-2 and MMP-9 in cultured annular pad cells of the chick lens17
and in human capsular bags.18
Induction of the proforms of both MMP-2 and MMP-9 has also been reported during hydrogen peroxide-induced cataract formation and after sham cataract surgery.19
Multiple MMPs and tissue inhibitors of matrix metalloproteinases have also been detected in the extracellular matrix and LECs from human capsules derived from post-cataract/intraocular lens surgery tissue, whereas normal anterior lens capsules did not.20
Evidence that MMP-2 may have an active role in mediating the EMT that occurs in ASC has been provided by findings in which overexpression of MMP-2 in the human HLE B-3 lens cell line caused a conversion of the cells into a myofibroblastic phenotype.16 The ability of other MMPs to induce LEC conversion, however, has not been investigated. In addition, the requirement for MMPs in mediating the formation of the TGFß-induced ASC plaques in the ex vivo lens and the mechanism(s) by which MMPs participate in ASC formation are not known. Investigations in other systems, including cancer, have shown that MMPs promote EMT by altering the E-cadherin/ß-catenin pathway through a phenomenon known as E-cadherin shedding.21-24 Specifically, the association between E-cadherin and ß-catenin is vulnerable to enzymatic attack by multiple MMPs, including MMP-9 and MMP-2, resulting in the formation of E-cadherin extracellular domain fragments with reported sizes ranging from 50 to 84 kd.24-26 Induction of MMPs by TGFß in the lens may lead to the EMT of LECs through a specific disruption in E-cadherin.
In the current study we directly tested the requirement of MMP activity in TGFß-induced ASC formation using a well-established ex vivo rat lens model27 in conjunction with the two different MMP inhibitors (MMPIs), the broad spectrum inhibitor GM6001 and a MMP-2/9-specific inhibitor (MMPI2/9). Additional experiments examined the capacity of these MMPIs to prevent decreases in optical quality of the lens induced by TGFß and to affect the cell adhesion molecule E-cadherin. Together, the findings of this study demonstrate that co-culture of lenses with two different MMPIs effectively suppressed TGFß-induced ASC formation and suggest attenuation of E-cadherin as a possible mechanism.
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Materials and Methods |
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The previously established TGFß-induced rat cataract model was used for these studies.27 Briefly, lenses were obtained from adult male Wistar rats and cultured overnight in 3.5 ml of serum-free M199 medium supplemented with 50 IU/ml penicillin, 50 µg/ml streptomycin, and 2.5 µg/ml fungizone (Invitrogen, Burlington, ON, Canada). The following day, lenses were either left untreated or treated with TGFß-2 (R&D Systems, Minneapolis, MN) at a final concentration of 1 or 2 ng/ml. Some lenses were co-treated with TGFß-2 and the broad spectrum MMP inhibitor GM6001 (Ilomastat) (Chemicon International, Temecula, CA) at concentrations ranging from 10 to 25 µmol/L or the MMP-2/9 inhibitor ((2R)-[(4-biphe-nylylsulfonyl)amino]-N-hydroxyl-3-phenylpropionamide) (Chemicon) at concentrations of 10 or 25 µmol/L. The GM6001 negative control (N-t-butoxycarbonyl-L-leucyl-L-tryptophan methylamide) (25 µmol/L) (Calbiochem, San Diego, CA) was also used. Lenses were subjected to optical analysis at subsequent time points of 2, 4, or 6 days and photographed using a digital camera mounted to a dissecting scope. The lenses were then fixed for histology and immunofluorescence or subjected to laser capture microdissection (LCM). The conditioned media were also collected from each treatment group for zymography and Western blot analysis.
Optical Analysis
Lens optical qualities (the average back vertex distance [BVD]) and sharpness of focus (BVD error) were assessed using the automated laser scanning system that was developed at the University of Waterloo.28-30
This system consists of a single collimated scanning helium-neon laser source that projects a thin (0.05 mm) laser beam onto a plain mirror mounted at 45° on a carriage assembly. A digital camera captures the actual position and slope of the laser beam at each step. When all steps have been made (20 beam positions across each lens), the captured data for each step position are used to calculate the back vertex distance. BVD (in millimeters) is defined as the measurement of the distance from the surface of the lens to the focal point where the laser beam crosses the optical axis of the lens being scanned. When portrayed graphically, the average BVD for the lenses is plotted for each eccentric position (Figure 1)
. With less spherical aberration, the data points line up as a straight line. The poorer the quality of the lens, the greater the variation in BVD (BVD error) is with eccentricity, as shown for lenses treated with TGFß (Figure 1)
. Because BVD error is a more sensitive measure of lens damage, the results are expressed in terms of BVD error.28-30
Repeated-measures analysis of variance was determined using SPSS 11.0 statistical software and was used to assess treatment, concentration of TGFß, and temporal effects on the back vertex variability for the first experiment in Figure 2
. One-way analysis of variance was used for analyzing data in the second experiment in Figure 3
, which examines the dosage effect of GM6001 on TGFß-induced BVD error at a single time point. A probability value (P) 
0.05, indicating a 95% confidence interval, was considered significant.
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Lenses were collected from different treatment groups and fixed overnight in an acetic acid:ethanol solution (1:99), dehydrated, embedded in paraffin, and processed for routine histology. Immunofluorescence was performed on 5-µm-thick paraffin-embedded sections. Sections were incubated with primary antibody specific for -SMA (1:100; Sigma, Oakville, ON, Canada) for 1 hour at room temperature, and bound primary antibodies were visualized with a fluorescein-isothiocyanate anti-mouse secondary antibody (1:50; Jackson ImmunoResearch Laboratories, West Grove, PA). All sections were mounted in Vectashield mounting medium with 4',6-diaminodino-2-phenylindol (Vector Laboratories, Burlington, ON, Canada) to visualize the nuclei.
Zymography
Conditioned media from all treatment groups were concentrated using 3.5-ml 10K Microsep concentrating devices (Viva Sciences, Hanover, Germany). Before concentration, refrigerated media were warmed to 37°C. The media were centrifuged at 1000 x g (at room temperature) for 5 minutes to pellet any debris before loading. Each device was loaded with an equal volume of supernatant, and the concentration was performed by centrifugation at 25°C for 30 minutes at 3000 x g. An equal volume of each concentrate was electrophoresed on 10% SDS-polyacrylamide gels containing 0.1% gelatin as the substrate. After electrophoresis, the gels were developed as described previously31 and stained in 0.5% Coomassie brilliant blue for 1 hour followed by destaining with 10% isopropanol. Sites of gelatinase activity were detected as clear bands against a background of uniform staining, which was digitally photographed.
Western Blot Analysis
Concentrated samples derived from the conditioned media were also examined by Western blot analysis. Equal volumes of sample were electrophoresed on a 10% SDS-polyacrylamide gel. The resolved bands were electro-transferred onto a nitrocellulose membrane (Pall Corporation, Pensacola, FL). Membranes were blocked with 5% skimmed milk powder in Tris-buffered saline (50 mmol/L Tris base and NaCl [pH 8.5]) and 0.1% Tween-20 and then incubated overnight at 4°C with primary antibodies generated against MMP-9 (1:500; Chemicon International), MMP-2 (1:500; Chemicon), or E-cadherin (1:1500; BD Transduction Laboratories). After this incubation, membranes were probed with an horseradish peroxidase-conjugated secondary antibody (1:7500; Amersham Biosciences, Little Chalfont, Buckinghamshire, UK) and ECL detection reagents (Amersham Biosciences). The Western blots were scanned and analyzed by image quantification software (ImageJ; National Institutes of Health, Bethesda, MD). The relative density versus control ratio was estimated using GraphPad Prism Software (GraphPad). Quantitative data were analyzed statistically using a Student’s t-test and expressed as mean ± SEM. A value of P < 0.05 was considered significant.
Laser Capture Microdissection, RNA Extraction, and cDNA Synthesis
After treatment, lenses were placed in a cryostat mold containing Tissue-Tek OCT (Sakura Finetek, Torrance, CA), frozen on dry ice, and then stored at –70°C. The frozen tissue was then sectioned at 5 to 8 µm in a cryostat, mounted on noncoated clean glass slides, and stored again at –70°C. Immediately before LCM, the frozen sections were thawed for 10 seconds and then stained with HistoGene LCM Frozen Section Staining kit (Arcturus, Mountain View, CA) using the protocol provided, with strict adherence to RNase-free conditions. LCM was then performed using the PixCell II (Arcturus), as described by others.32,33 The HistoGene stain allowed for the identification of the general morphology of the epithelium. Cells from the epithelial region of the lens were then captured on CapSure Macro LCM Caps (Arcturus) using the PixCell II LCM Microscope (Arcturus) with a minimal beam diameter of 7.5 µm. Total cellular RNA was then extracted from lifted cells using the PicoPure RNA Isolation kit (Arcturus). Purified RNA was analyzed both qualitatively and quantitatively using an Agilent 2100 Bioanalyzer (Agilent Technologies, Foster City, CA) and subjected to standard reverse transcription reactions (SuperScript II; Life Technologies).
Quantification of Gene Expression Using Real Time Quantitative Polymerase Chain Reaction (RT-QPCR)
E-cadherin and -SMA gene expression from recovered cDNA was analyzed with RT-PCR using a 96-well TaqMan optical reaction plate format on an ABI Prism 7700 sequence detection system (Applied Biosystems, Foster City, CA). RNA was normalized to 18S and GAPDH for each reaction. Each 25-µl PCR reaction (including controls) contained TaqMan Universal Master Mix (Applied Biosystems), gene-specific forward and reverse primers (Mobix, Hamilton, ON, Canada), and probes for target and endogenous control genes (Applied Biosystems). Serial dilutions (one- to fivefold) of standard samples were prepared in separate wells in duplicate for endogenous control genes (18S and GAPDH), E-cadherin, and -SMA gene targets. Standard and unknown samples were added in a volume of 5 µl. Thermal cycling parameters consisted of the following: 2 minutes at 50°C, 10 minutes at 95°C followed by 40 cycles of 15 seconds at 95°C, and 1 minute at 60°C. The number of target gene copies was calculated from a standard curve generated in parallel with each batch of samples. A linear relationship was detected over at least 5 orders of magnitude. In all experiments, the correlation coefficient was between 0.997 and 0.986. The normalization of samples was performed by dividing the number of copies of both E-cadherin and -SMA by the number of copies of 18S and GAPDH. PCR reactions for E-cadherin and -SMA cDNA quantification were performed using standard cDNA dilution curves. Quantitative data were analyzed statistically using a Student’s t-test and expressed as means ± SEM. A value of P < 0.05 was considered significant.
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Results |
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In the following experiments, the broad spectrum MMP inhibitor GM6001 was used in the rat subcapsular cataract model to determine whether it could effectively suppress TGFß-induced subcapsular cataract formation. To perform these experiments, excised rat lenses were treated with exogenous TGFß2 for a period of 6 days. The TGFß-treated lenses exhibited multiple, distinct opacities on the anterior surface of the lens, as previously described27,34
(Figure 4B)
, whereas the untreated control lenses remained transparent and devoid of opacities (Figure 4A)
. In comparison with lenses treated with TGFß alone, lenses co-cultured with TGFß and GM6001 (25 µmol/L) for the 6-day period did not exhibit visible subcapsular cataracts (Figure 4C)
but resembled the control lenses. Histological cross-sections of the lenses treated with TGFß revealed the presence of numerous plaques consisting of a multilayering of cells beneath the lens capsule (Figure 4F)
in contrast to the simple cuboidal monolayer of epithelial cells observed in the control lens (Figure 4E)
. Strong immunoreactivity of -SMA was observed in a proportion of the cells of the subcapsular plaques in the TGFß-treated lenses (Figure 4F)
. In comparison, the lenses co-cultured with TGFß and GM6001 (25 µmol/L) did not exhibit multilayering of the lens epithelium and no -SMA immunoreactivity was observed under the same immunolocalization conditions as outlined above (Figure 4G)
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-SMA expression. Lenses co-treated with TGFß and the MMP-2/9-specific inhibitor (10 µmol/L) exhibited slight multilayering of the epithelium (two layers observed) in some regions of the lens with very faint -SMA immunoreactivity (data not shown). However, lenses co-treated with TGFß and the MMP-2/9 inhibitor (25 µmol/L) resembled control lenses and did not exhibit -SMA immunoreactivity (Figure 4, D and H)
. Note that lenses treated with each of the MMPIs, in the absence of TGFß, resembled the control lenses (not shown).
A laser scanning system (ScanTox) was next used to determine quantitative differences in the optical quality of the lenses from three different treatment groups (control, TGFß, and TGFß plus 25 µmol/L GM6001) at three time points: days 2, 4, and 6. Analyses of BVD errors showed that there was both a treatment and temporal effect of TGFß on the cultured rat lens (Figure 2)
. Although the BVD errors for the control lenses did not change significantly from day 0 to 6, treatment with either 1 or 2 ng/ml of TGFß caused a significant increase in BVD error by day 6 of the time course. In contrast, the groups of lenses co-treated with TGFß (at 1 and 2 ng/ml) and GM6001 (25 µmol/L) did not exhibit a significant change in BVD error over the 6-day period. Statistical differences between the treatment groups at each time point were also determined. At days 0, 2, and 4, no significant difference in BVD error was observed between all groups (Figure 2)
. However, at day 6, both of the TGFß-treated groups of lenses (1 and 2 ng/ml) exhibited a significantly larger BVD error versus the control group and the GM6001 co-treated lenses (Figure 2)
. Lenses cultured with the inhibitor alone did not exhibit changes in BVD error relative to untreated lenses (not shown).
A dose-dependent effect of the broad spectrum inhibitor GM6001 in preventing the TGFß-induced cataracts was also observed. Similar to earlier experiments, treatment with TGFß for 6 days resulted in BVD errors that were statistically greater than control lenses. Similarly, those lenses co-treated with TGFß and GM6001 (10 or 15 µmol/L) significantly differed from controls and exhibited cataracts. In contrast, lenses co-treated with higher concentrations of GM6001, such as 20 and 25 µmol/L, exhibited BVD errors that were very similar to the controls, further demonstrating the dramatic suppression in cataract formation (Figure 3)
. Treatment with an analog of GM6001, with no MMP inhibitory activity (negative control), exhibited a similar BVD error to the TGFß-treated lens, indicating that the effect of GM6001 on maintaining lens optical quality was related to its MMP inhibitory activity.
TGFß-Induced ASC Formation Is Accompanied by Enhanced Secretion of MMP-2 and MMP-9
To examine the timing and level of induction of MMPs in the rat lens after TGFß treatment and subcapsular cataract formation, zymography was performed on conditioned media of lenses taken at the 6-day time point from the following treatment groups: control, TGFß (2 ng/ml), TGFß (2 ng/ml) plus GM6001 (25 µmol/L), and TGFß (2 ng/ml) plus the MMP-2/9-specific inhibitor (25 µmol/L). Conditioned media from all treatment groups exhibited distinct bands on gelatin gels, indicating the presence of MMPs with gelatinolytic and/or collagenolytic activity (Figure 5A)
. Conditioned media from control lenses exhibited expression of a 92-kd band, corresponding to the proform of MMP-9.11
In comparison with control lens media, media from lenses treated with TGFß exhibited additional bands of 62, 65, and 72 kd, corresponding to the active and proforms of MMP-2;11
MMP-9 levels were also increased. Media obtained from lenses co-treated with TGFß and either GM6001 or MMP-2/9 inhibitor for 6 days exhibited reduced levels of all gelatinolytic bands relative to that of TGFß-treated lenses (Figure 5A)
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. In comparison, conditioned media from the lenses co-cultured with TGFß plus GM6001 or TGFß plus the MMP-2/9 inhibitor showed undetectable levels of MMP-2 similar to control lenses (Figure 5B)
. Blots probed with the MMP-9-specific antibody revealed a band at 92 kd, corresponding to the proform of MMP-9 (Figure 5B)
. Similar to the zymography results, constitutive MMP-9 protein expression was evident in the conditioned media of control lenses. In comparison, media from lenses treated with TGFß exhibited significantly higher levels of MMP-9 after 2, 4, and 6 days of treatment (Figure 5, B and C)
. Co-treatment with TGFß and GM6001 revealed significant attenuation of MMP-9 in conditioned media relative to TGFß treatment alone at all three time points, whereas conditioned media from co-treatment of TGFß and the specific MMP-2/9 inhibitor showed a significant attenuation of MMP-9 at days 2 and 4 only (Figure 5, B and C)
. Note that lenses treated with either MMPI alone exhibited levels of MMP-9 and MMP-2 similar to that of control lenses (data not shown). MMPIs Attenuate TGFß-Induced E-Cadherin Shedding in the Rat Lens
Because previous studies had shown the ability of MMPs to induce E-cadherin shedding,22-24
the following experiments were designed to determine whether TGFß treatment of the rat lens results in an induction of E-cadherin shedding and whether this can be modulated by the MMPIs. To accomplish this, previously concentrated conditioned media were obtained from the 2-, 4-, and 6-day treatment groups outlined earlier and subjected to Western blot analysis using an antibody specific for the extracellular domain of E-cadherin, which has been used in previous studies to detect the presence of soluble E-cadherin fragments. An E-cadherin fragment of approximately 72 kd was detected in the conditioned media from lenses treated with TGFß for 6 days and was not observed in media from untreated lenses (Figure 6A)
. Media from lenses treated with TGFß for shorter time periods (days 2 and 4) did not exhibit detectable levels of the fragment (not shown). Importantly, a significant twofold reduction in the levels of this fragment was detected in media from lenses co-treated with TGFß and the broad spectrum inhibitor GM6001, whereas levels were undetectable in media from lenses co-treated with TGFß and the MMP-2/9 inhibitor (Figure 6A)
. Lenses treated with either of the MMPIs alone also exhibited undetectable levels of the E-cadherin fragment (data not shown). Thus, co-culture of lenses with either of the MMPIs tested resulted in attenuated levels of TGFß-induced E-cadherin fragment.
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, its expression, relative to that of GAPDH, was suppressed nearly fourfold in the plaque tissue of TGFß-treated lenses. In comparison, E-cadherin levels in cells from the epithelial region of lenses co-treated with TGFß and GM6001 (25 µmol/L) were significantly higher (6.9-fold) than those treated with TGFß alone and significantly greater than that of controls. Treatment with 25 µmol/L GM6001 alone also produced a significant increase in E-cadherin when compared with control lenses (P < 0.01) (2.5-fold). When these experiments were performed using the 18S housekeeping gene, data exhibited the same trends seen with GAPDH (not shown). Thus, treatment of lenses with GM6001 prevented the attenuation of E-cadherin mRNA induced by TGFß and further increased the constitutive levels of E-cadherin mRNA.
For comparison, we also examined the levels of -SMA mRNA in the same tissues used to perform the E-cadherinstudies (Figure 6C)
. These experiments revealed little to no expression of -SMA mRNA in the cells obtained from the epithelial region of control lenses whereas its expression, relative to that of GAPDH, was significantly induced in the plaque tissue of TGFß-treated lenses. This corresponds with our earlier experiments, which showed increased -SMA protein immunoreactivity in the subcapsular plaques (Figure 4F)
. -SMA mRNA levels in the LECs co-treated with the MMPI were substantially reduced compared with the plaque cells of TGFß-treated lenses, demonstrating that co-culture with the MMPI resulted in suppression in induction of -SMA by TGFß. Addition of GM6001 (25 µmol/L) alone to lenses did not alter -SMA mRNA levels when compared with those of control lenses (Figure 6C)
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Discussion |
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TopAbstractMaterials and MethodsResultsDiscussionReferences |
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-SMA-expressing cells indicative of the EMT of LECs. Further evidence is provided to show that treatment of the rat lens with TGFß resulted in appearance of proteolytic fragments of the cell-cell adhesion molecule E-cadherin, an event that was attenuated by co-treatment with either of the two MMPIs tested. Together, these data suggest that the suppression of TGFß-mediated E-cadherin shedding and degradation is a possible mechanism by which MMPIs reduce the appearance of ASC plaques.
Earlier work has shown that MMP-2 expression is induced in the rat lens after treatment with TGFß.16
Furthermore, overexpression of MMP-2 via stable transfection results in conversion of cultured human LECs into -SMA-expressing cells.16
Similar to these findings, we also report induced secretion of MMP-2 in the rat lens after treatment with TGFß, accompanied by enhanced secretion of MMP-9. A recent study revealed that MMP-9 and MMP-2 mRNA are expressed in the normal rat lens epithelium.35
This suggests that constitutive mRNA expression of these MMPs does not result in the EMT of LECs and cataract formation. However, induced levels of MMP-2 and/or MMP-9, above those of constitutive expression, and accompanying secretion of MMPs could result in EMT in the lens. Induced levels of MMP-2 and membrane-type 1-MMP have been correlated with EMT in the embryonic heart, and overexpression of MMP-3 has been shown to cause conversion of mammary epithelial cells into mesenchymal cells.21,36
The fact that co-treatment with GM6001 and the specific MMP2/9 inhibitor resulted in suppression of TGFß-induced -SMA expression in the rat lens, further corroborates these earlier findings and suggests that MMPs are important for mediating the EMT of LECs. We further show that treatment with MMPIs led to a substantial reduction in subsequent plaque formation in the ex vivo lens and maintenance of lens optical quality. Because MMP-2 and MMP-9 were induced after TGFß treatment and the MMPIs used have inhibitory activity against both, it cannot be discerned whether one or both of these MMPs participate in ASC formation. Future studies that target the individual expression of MMP-2 or MMP-9, such as through gene knockdown experiments or the use of specific MMP-2 and MMP-9 knockout mouse models, will help to further determine whether one or both of these MMPs is critical for ASC formation.
MMPs are principally known for their role in extracellular matrix (ECM) remodeling.11 However, additional roles for MMPs have emerged, including their ability to regulate cell migration, invasion, and EMT.21,36 It has been suggested that MMPs may contribute to EMT by participating in the separation of epithelial cells from their basement membrane,37,38 thereby promoting their migration during cellular transformation.7,37,38 However, more recent findings suggest that MMPs participate in the initial activation stages of EMT through dissociation of the E-cadherin/ß-catenin complex.22-24 Proteolytic cleavage of the N-terminal extracellular domain of E-cadherin by MMPs, referred to as "E-cadherin shedding," results in the formation of an E-cadherin extracellular domain fragment with reported sizes ranging from 50 to 84 kd, compared with the intact 120-kd protein.39 Here, we report the appearance of a 72-kd E-cadherin fragment in the conditioned media of lenses treated with TGFß that was not detected in the media from untreated lenses. To the best of our knowledge, this is the first report of E-cadherin shedding in a cataract model. The appearance of the E-cadherin fragment in the TGFß-treated rat lenses was also shown to coincide with enhanced levels of secreted MMP-2 and MMP-9, MMPs that have been implicated in E-cadherin shedding and degradation in other systems.24,40 We further demonstrated that the TGFß-induced levels of the E-cadherin fragment were attenuated by co-treatment with either MMPI, broad spectrum GM6001, or MMP-2/9 inhibitor, suggesting that TGFß-induced fragmentation of E-cadherin is mediated by MMP activity. Interestingly, the ability of GM6001 to suppress E-cadherin shedding has been shown in nitric oxide-treated murine colonic epithelial cells, and this led to further stabilization of E-cadherin junctions.22,24
Along with E-cadherin shedding, we also reported a significant decrease in the E-cadherin mRNA levels in the subcapsular plaque tissue of TGFß-treated lenses. Decreased E-cadherin expression has also been reported after dexamethasone-induced cataract formation in the cultured rat lens; however, evidence of E-cadherin shedding in this cataract model was not investigated.41
Cadherins are known to act as cell signaling receptors by controlling the localization of ß-catenin. Proteolytic shedding of E-cadherin causes dissociation of ß-catenin from membrane-bound E-cadherin, resulting in increases in the levels of free ß-catenin in the cytoplasm.24
ß-Catenin can then localize to the nucleus where it associates with the T-cell factor/lymphoid enhancer factor(s) to activate or repress target gene expression.22
Thus, the changes in E-cadherin and -SMA mRNA levels that we observed may be associated with changes in ß-catenin localization. The levels of E-cadherin mRNA in lenses co-treated with TGFß and GM6001 were found to be well above that of control lenses, and treatment of lenses with GM6001 alone also resulted in a substantial elevation in the constitutive amount of E-cadherin mRNA. These findings suggest that E-cadherin mRNA expression was either stimulated or stabilized by GM6001.
The findings of the current study also revealed that treatment with the broad spectrum inhibitor GM6001 suppressed TGFß-induced levels of both MMP-2- and MMP-9-secreted protein, whereas the MMP-2/9 inhibitor specifically blocked MMP-2 induced levels. The primary function of both MMPIs is to inhibit MMP enzymatic activity. However, MMPIs such as GM6001 have been shown to have an inhibitory effect on MMP expression in other systems, yet this has remained unexplained.42,43
Previous work has shown that the myofibroblast-like cells in the subcapuslar plaques of rat lenses treated with TGFß exhibited immunoreactivity for both -SMA and MMP-2.16
Thus, the absence of MMP-2-secreted protein in the media from lenses co-treated with either of the two MMPIs may simply be due to the fact that both MMPIs suppress the appearance of myofibroblasts, the cell type that expresses MMP-2.
In summary, the findings of the current study have shown that treatment with the broad spectrum MMP inhibitor GM6001 and the specific MMP2/9 inhibitor significantly suppressed the formation of TGFß-induced ASC plaques in the rat lens. This suppression was associated with decreased -SMA expression and a significant decrease in the 72-kd soluble E-cadherin fragment, indicative of E-cadherin shedding. Together, these data suggest the novel finding that MMPIs suppress the EMT of LECs in ASC formation through inhibition in MMP-mediated disruption of E-cadherin. Based on these findings, two possible therapeutic strategies for the prevention of ASC formation can be proposed: inhibition of MMP activity and/or attenuation of aberrant E-cadherin shedding.
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Acknowledgements |
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Footnotes |
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Supported by research grants from the National Institutes of Health project grants (EY013845-01 and EY015006-01 to J.A.W.-M.), Canada Foundation for Innovation (to J.A.W.-M.), and Natural Sciences and Engineering Research Council of Canada (to J.G.S.). J.A.W.-M. was a Research to Prevent Blindness Career Development Awardee (2000–2003).
D.J.D. and G.P. contributed equally to this study.
Accepted for publication August 30, 2005.
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A. Banh, P. A. Deschamps, J. Gauldie, P. A. Overbeek, J. G. Sivak, and J. A. West-Mays Lens-Specific Expression of TGF-{beta} Induces Anterior Subcapsular Cataract Formation in the Absence of Smad3. Invest. Ophthalmol. Vis. Sci., August 1, 2006; 47(8): 3450 - 3460. [Abstract] [Full Text] [PDF]
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