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Published online before print October 2, 2008
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Biological Perspectives |
From the Mucosal Biology Research Center and Center for Celiac Research, University of Maryland School of Medicine, Baltimore, Maryland
Abstract
The anatomical and functional arrangement of the gastrointestinal tract suggests that this organ, beside its digestive and absorptive functions, regulates the trafficking of macromolecules between the environment and the host through a barrier mechanism. Under physiological circumstances, this trafficking is safeguarded by the competency of intercellular tight junctions, structures whose physiological modulation is mediated by, among others, the recently described protein zonulin. To prevent harm and minimize inflammation, the same paracellular pathway, in concert with the gut-associated lymphoid tissue and the neuroendocrine network, controls the equilibrium between tolerance and immunity to nonself antigens. The zonulin pathway has been exploited to deliver drugs, macromolecules, or vaccines that normally would not be absorbed through the gastrointestinal mucosal barrier. However, if the tightly regulated trafficking of macromolecules is jeopardized secondary to prolonged zonulin up-regulation, the excessive flow of nonself antigens in the intestinal submucosa can cause both intestinal and extraintestinal autoimmune disorders in genetically susceptible individuals. This new paradigm subverts traditional theories underlying the development of autoimmunity, which are based on molecular mimicry and/or the bystander effect, and suggests that the autoimmune process can be arrested if the interplay between genes and environmental triggers is prevented by re-establishing intestinal barrier competency. Understanding the role of zonulin-dependent intestinal barrier dysfunction in the pathogenesis of autoimmune diseases is an area of translational research that encompasses many fields.
3.5 kDa) will be excluded from this uptake route. Macromolecule trafficking is dictated mainly by intestinal paracellular permeability, the regulation of which depends on the modulation of intercellular tight junctions (TJs). A century ago, TJs were conceptualized as a secreted extracellular cement forming an absolute and unregulated barrier within the paracellular space. The contribution of the paracellular pathway of the GI tract to the general economy of molecule trafficking between environment and host, therefore, was judged to be negligible. It is now apparent that TJs are extremely dynamic structures involved in several key functions of the intestinal epithelium both under physiological and pathological circumstances. TJ modulation has also been exploited for the delivery of drugs and macromolecules normally not absorbed through the GI tract. Given the structural and functional characteristics of intercellular TJs and the protean nature of the intestinal content, the gut mucosa represent the battlefield where friends (ie, nutrients and enteric microflora) and foes (ie, pathogenic microorganisms and their toxins) must be selectively recognized to reach an ideal balance between tolerance and immune response to nonself antigens. Antigen trafficking through TJs and their sampling by the gut-associated lymphoid tissue (GALT) are among the key strategies applied to reach this balance. This review is mainly focused on the role of zonulin, a modulator of intercellular TJs, on the pathophysiology of intestinal permeability and its use for therapeutic applications. For a more complete overview on the topic, the reader is referred to an excellent review article recently published in The American Journal of Pathology.1
Structural Composition of Intercellular TJs
The intercellular TJs are complex structures composed by a multiprotein complex anatomically and functionally connected with the underlying apical actomyosin ring (Figure 1)
. Among the TJ proteins identified so far are the transmembrane proteins occludin, the claudins, and junctional adhesion molecules (JAMs), and cytoplasmic plaque proteins ZO-1, ZO-2, ZO-3, cingulin, and 7H6.2
In vitro, the electrical resistance is a measure of charge flow across the membrane and thus reflects the permeability of the paracellular shunt pathway and the tightness of the intercellular TJ. TJ assembly is the result of a complex cascade of biochemical events that ultimately lead to the formation of an organized network of TJ elements, the composition of which has been only partially characterized. An in depth discussion of the complexity of TJ composition, the protein-protein interactions that govern their assembly and competency, and the multiple signaling pathways involved in the orchestration of all these events goes beyond the scope of this review. Therefore, only a general overview of the TJ structural composition will be covered herein to provide proper background and rationale to the main focus of the review.
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To date, multiple proteins that make up the TJ strands have been identified and include occludin3 and members of the claudin family,4 a group of at least 20 tissue-specific proteins. The JAMs, proteins that belong to the Ig superfamily, have been described as additional components of the TJ fibrils.5 JAMs reportedly bind to ZO-1, aiding its localization to the junctional complex.6
Occludin is a transmembrane TJ phosphoprotein of 65 kDa.7
It is expressed in TJs of both epithelial and endothelial cells. The proposed folding topology places both NH2 and COOH terminals within the cytoplasm, allowing the polypeptide to pass out and back inside twice within the NH2 terminal half.7
Occludin has recently been shown to function as a cell-cell adhesion molecule and appears to participate in maintaining the intramembrane diffusion barrier.8
Occludin was previously considered to be involved in establishing the seal at the sites of junctional strands.9
However, discrepancies between the presence of fibrils and occludin expression in several tissues, including human testis and endothelial cells, led to the discovery of the claudins as integral membrane TJ proteins.4
The claudins are 20- to 25-kDa tetraspan membrane proteins that each contain two extracellular loops with variably charged amino acid residues and short intracellular tails.9 The claudins determine the barrier properties of the paracellular pathway because they can completely tighten the intercellular space to solutes, and they can form paracellular ion pores. It is assumed that the extracellular loops specify these claudin functions.
JAMs are characterized by the molecular structure composed of two extracellular immunoglobulin loops, a single transmembrane domain, and a cytoplasmic domain with a PDZ-binding motif.5 JAMs interact with several ligands in both homophilic and heterophilic manners by the extracellular domain and also bind to various PDZ domain-containing proteins at the C terminus. Through these protein-protein interactions, JAMs are implicated in diverse biological functions at the TJ, including cell-cell adhesion, junctional assembly, regulation of paracellular permeability, and leukocyte transmigration.10,11
Cytoplasmic TJ Proteins
The cytoplasmic plaque of the TJ includes multiple proteins that have been defined at the molecular level as well as several others that await further characterization. Through interactions with each other and with cytoskeletal proteins, these scaffold elements (ZO-1, ZO-2, ZO3, and ZO-1-associated protein kinase or ZAK) functionally couple integral membrane TJ proteins to actin microfilaments.12
TJ plaque proteins also appear to be direct targets and effectors of different signaling pathways. Additional cytoplasmic plaque proteins have been localized to the TJ, including 7H6,9
Rab 13 and 3b,13
G
i-2,14
PKC,15
symplekin (126.5 kDa),16
ZA-1TJ, and cingulin (140-kDa phosphoprotein).17
GI Mucosal Immune System
The GALT
Paracellular passage of macromolecules, under either physiological or pathological circumstances, is safeguarded by the GALT (Figure 2)
. The GALT serves as a containment system preventing potentially harmful intestinal antigens from reaching the systemic circulation and induces systemic tolerance against luminal antigens by a process that involves polymeric IgA secretion and the induction of regulatory T cells. Another important factor for the intestinal immunological responsiveness is the major histocompatibility complex. Human leukocyte antigen (HLA) class I and class II genes are located in the major histocompatibility complex on chromosome 6. These genes code for glycoproteins, which bind peptides, and this HLA-peptide complex is recognized by certain T-cell receptors in the intestinal mucosa.18,19
Susceptibility to at least 50 diseases is associated with specific HLA class I or class II alleles.
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Innate and Adaptive Immunity and Their Interactions
Recognition of antigens by dendritic cells triggers a family of pattern recognition receptors, Toll-like receptors (TLRs), which change dendritic cell phenotype and function. TLRs are the major receptors involved in the discrimination between self and nonself based on the recognition of conserved bacterial molecular patterns (Figure 3)
. In intestinal epithelial cells, TLRs play a role in normal mucosal homeostasis and are particularly important in the interaction between the mucosa and the luminal flora.21
There are a number of TLRs, all of which are present in the gut and respond to different stimuli resulting in different adaptive immune responses.22-24
There is now evidence for a differential response to stimuli arising from TLRs located at the basolateral versus the apical surface.25
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The Zonulin System
To meet the diverse physiological challenges to which the intestinal epithelial barrier is subjected, TJs must be capable of rapid and coordinated responses. This requires the presence of a complex regulatory system that orchestrates the state of assembly of the TJ multiprotein network. Although knowledge about TJ ultrastructure and intracellular signaling events has progressed significantly during the past decade, relatively little is known about their pathophysiological regulation. The discovery of zonula occludens toxin (Zot), an enterotoxin expressed by Vibrio cholerae that reversibly opens TJs,29
has increased our understanding of the intricate mechanisms that regulate the intestinal epithelial paracellular pathway. Zot is a single polypeptide chain of 44.8 kDa, encoded by the cholera toxin bacteriophage (CTX
) present in toxigenic strains of V. cholerae.29
Zot action is mediated through a cascade of intracellular events that lead to PKC--dependent polymerization of actin microfilaments and subsequent TJ disassembly.30
Immunofluorescence binding studies have shown that Zot binding varies within the intestine, being detectable in the jejunum and distal ileum, but not in the colon, and decreasing along the villous-crypt axis.31
This binding distribution coincides with the differential intestinal epithelial barrier responsiveness and actin reorganization that occurs along the villous axis32,33
as well as with the regional effect of Zot on intestinal permeability.30,31
These combined data demonstrate that Zot regulates TJs in a rapid, reversible, and reproducible manner. Based on these observations, it has been postulated that Zot mimics an immunologically related, endogenous modulator of epithelial TJs. The combination of affinity-purified anti-Zot antibodies and the Ussing chamber assay allowed the identification of a human intestinal Zot homolog that was designated as zonulin (Figure 4)
.34
Affinity-purified zonulin reduced transepithelial electrical resistance compared to the media control in both monkey jejunum and ileum, but not in the colon.34
V. cholerae-derived Zot and human zonulin both act on intestinal TJs29,35,36
and display the same regional barrier responsiveness31
coincident with Zot receptor distribution within the intestine,30,33
These findings suggest that these two molecules could potentially interact with the same receptor(s). Comparison of the primary amino acid structures of Zot and zonulin provided insights into the structural requirements for ligand engagement of the receptor coupled to intestinal TJ regulation. The NH2-termini of zonulin and the Zot active fragment share a common octapeptide motif (GGVLVQPG) that is critical for intestinal receptor binding.34,36
The synthetic octapeptide motif, named AT1001, is an efficient inhibitor of both Zot and zonulin because it binds to the target receptor, but it does not activate the intracellular signaling leading to TJ disassembly and antigen trafficking.34,36
Zonulin cloning and characterization have revealed that it belongs to a family of serine proteases with structure similarities with a series of growth hormones, including epidermal growth factor (A. Fasano, unpublished).
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The physiological role of the zonulin system remains to be established; however, it is likely that this system is involved in several functions, including TJ regulation responsible for the movement of fluid, macromolecules, and leukocytes between body compartments. Another possible physiological role of zonulin is the protection against microorganism colonization.37 In the absence of enteric infections, the mammalian small intestine is virtually sterile. The colonization of the proximal gut by enteric microorganisms (even without apparent mucosal damage or elaboration of specific toxins) may lead to a leaky intestine.38 However, the mechanism(s) by which this disturbed physiological regulation of the intestinal TJ permeability secondary to proximal bacterial contamination occurs remains unclear. It has been reported that both normal enteric bacterial flora isolates (well characterized for not harboring any known pathogenic traits) and pathogenic bacteria induce alteration of the TJ competency as suggested by changes in epithelial resistance and increased passage of inulin.37 These changes were mirrored by the concomitant expression of zonulin in organ culture systems and occurred even when bacteria were killed by gentamicin treatment.37 These results suggest that the presence of enteric microorganisms in the small intestine, but not in the colon, where the zonulin system is not operative,29-31 induces a host-dependent mucosal response that leads to the luminal secretion of zonulin. The fact that the interaction of bacteria with the intestinal mucosa induces zonulin release, irrespective of their pathogenic traits or viability, can be interpreted as a bacteria-independent mechanism of defense of the host that reacts to the abnormal presence of microorganisms on the surface of the small intestine. After the zonulin-induced opening of TJs, water is secreted into the intestinal lumen after hydrostatic pressure gradients31 and bacteria are flushed out of the small intestine.
Pathology of the Zonulin System
Inappropriate activation of the zonulin pathway, both in terms of timing and duration, can lead to an excessive and unregulated paracellular passage of nonself antigens in the lamina propria. Intestinal microorganisms have been long claimed to be possibly involved in autoimmune disease pathogenesis.39
Therefore, it is possible to hypothesize that the same zonulin innate immunity response that protect us against proximal microorganism contamination can cause a loss of intestinal barrier function when this pathway is chronically or exaggeratedly stimulated by enteric bacteria and/or viruses. Nevertheless, the environmental triggers of autoimmunity remain elusive for all autoimmune diseases except celiac disease (CD), for which gluten, the staple protein of wheat, has been identified as the undisputable trigger.40
We have demonstrated that, like bacterial colonization,37
gluten and its toxic component gliadin activate the zonulin pathway by "mistake of evolution."41
Recently we have shown that gliadin binds to CXCR3 on epithelial cells to initiate an increase in intestinal permeability through a MyD88-dependent release of zonulin that enables the paracellular passage of gliadin (and possibly other nonself antigens) from the intestinal lumen to the gut mucosa.42
In genetically predisposed individuals, gliadin may attract and stimulate other CXCR3-expressing cells, including 
T cells, CD3+CD8+β T cells, and NK cells, leading to the early activation of the innate immune arm of the CD inflammatory response.42
Role of Zonulin in Autoimmune Diseases
Role of Intestinal Permeability in Autoimmune Disease Pathogenesis
Intestinal TJ dysfunction occurs in a variety of clinical conditions, including food allergies, infections of the GI tract, autoimmune diseases, and inflammatory bowel disease (IBD).43 Healthy, mature gut mucosa with its intact TJ serves as the main barrier to the passage of macromolecules. During the healthy state, small quantities of immunologically active antigens cross the gut host barrier. These antigens are absorbed across the mucosa through at least two pathways. The vast majority of absorbed proteins (up to 90%) crosses the intestinal barrier via the transcellular pathway, followed by lysosomal degradation that converts proteins into smaller, nonimmunogenic peptides. These residual peptides are transported as intact proteins, through the paracellular pathway; it involves a subtle but sophisticated regulation of intercellular TJ that leads to antigen tolerance. When the integrity of the TJ system is compromised, a deleterious immune response to environmental antigens may be elicited. Several autoimmune diseases are characterized by loss of intestinal barrier function,43 suggesting a role of altered intestinal permeability in the pathogenesis of autoimmune diseases.
Specific Autoimmune Diseases in Which Zonulin Involvement Has Been Proved or Hypothesized
CD: CD represents the best testimonial of this theory. CD is a unique model of autoimmunity in which, in contrast to most other autoimmune diseases, a close genetic association with HLA genes, a highly specific humoral autoimmune response against tissue transglutaminase, and, most importantly, the triggering environmental factor (gliadin) are all known. Early in the disease, TJs are opened44,45
and severe intestinal damage ensues45
(Figure 5)
. The up-regulation of the zonulin innate immunity pathway is directly induced by the exposure to the disease’s antigenic trigger gliadin.46
To determine whether zonulin production is perturbed during the acute phase of CD, intestinal tissues from patients with active CD and non-CD controls were probed for zonulin expression.47
Quantitative immunoblotting of intestinal tissue lysates from active CD patients confirmed the increase in zonulin protein compared to control tissues.47
Zonulin up-regulation during the acute phase of CD was confirmed by measuring zonulin concentration in sera of 189 CD patients using a sandwich enzyme-linked immunosorbent assay. Compared to healthy controls, CD patients in the acute phase of the disease had higher zonulin serum concentrations that decreased after adherence to a gluten-free diet.47
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. The key role of gliadin-induced zonulin release and subsequent loss of intestinal barrier function in CD pathogenesis has been supported by clinical trials with the zonulin inhibitor AT1001.50 Gliadin interaction with macrophages initiates signaling through a TLR-like pathway, resulting in the establishment of a pro-inflammatory (Th1-type) cytokine milieu that results in mononuclear cell infiltration into the submucosa. This, in turn, may permit the interaction of T cells with antigen-presenting cells, including macrophages, leading ultimately to the antigen-specific adaptive immune response seen in patients with CD. Once gluten is removed from the diet, serum zonulin levels decrease, the intestine resumes its baseline barrier function, the autoantibody titers are normalized, the autoimmune process shuts off, and consequently, the intestinal damage (that represents the biological outcome of the autoimmune process) heals completely.
IBDs: The pathogenesis of IBD remains unknown, although in recent years there is convincing evidence to implicate genetic, immunological, and environmental factors in initiating the autoimmune process. Several lines of evidence, however, suggest that an increased intestinal permeability plays a central role in the pathogenesis of IBD.39,51
Like CD, IBD may be related to an innate immune deficiency, leading to the inappropriate access of nonself antigens to the GALT. In clinically asymptomatic Crohn’s disease patients, increased intestinal epithelial permeability precedes clinical relapse by as much as 1 year, suggesting that a permeability defect is an early event in disease exacerbation.51
The hypothesis that abnormal intestinal barrier function is a genetic trait involved in the pathogenesis of IBD is further supported by the observation that clinically asymptomatic first-degree relatives of Crohn’s disease patients may have increased intestinal permeability.39,51
We have recently generated evidence suggesting that zonulin up-regulation is detectable in the acute phase of IBD and that its serum levels decrease (but are still higher than normal) once the inflammatory process subsides after specific treatment (Bai J. and Fasano A., unpublished data). Although a primary defect of the intestinal barrier function (possibly secondary to activation of the zonulin pathway) may be involved in the early steps of the pathogenesis of IBD, the production of cytokines, including interferon- and tumor necrosis factor-, secondary to the inflammatory process serve to perpetuate the increased intestinal permeability by reorganizing TJ proteins ZO-1, JAM 1, occludin, claudin-1, and claudin-4.52-55
In this manner, a vicious cycle is created in which barrier dysfunction allows further leakage of luminal contents, thereby triggering an immune response that in turn promotes further leakiness.
Type 1 Diabetes (T1D): The trigger of the autoimmune destruction of pancreatic β cells in T1D is unknown. T1D has the same pathogenic challenges as other autoimmune diseases: defining the environmental triggers and how these triggers cross the intestinal barrier to interact with the immune system.43
It is the interplay between environmental factors and specific susceptibility genes that dictates the aberrant immune response responsible for the onset of disease. Less than 10% of those with increased genetic susceptibility progress to clinical disease, suggesting a strong environmental trigger in the prediabetic process. Environmental factors are also likely affecting the outcome of the process and the rate of progression to disease in those who develop T1D. One theory is that antigens absorbed through the gut may be involved. The specific cells that are key for this immune response lie in close proximity to the intestinal epithelial barrier (Figure 2)
.56,57
Another critical factor for intestinal immunological responsiveness is the major histocompatibility complex. Certain HLA class II alleles account for 40% of the genetic susceptibility to T1D in Caucasians; however, the majority of genetically predisposed individuals do not develop T1D. This supports the concept that reaction to some environmental products triggers autoimmune destruction of β cells and leads to T1D. Recent studies have shown that altered intestinal permeability occurs in T1D before the onset of complications,58,59 which is not the case in type 2 diabetes.60 This has led to the suggestion that increased intestinal permeability attributable to alterations in intestinal TJs is responsible for the onset of T1D.59,61,62 This hypothesis is supported by a recent study performed in an animal model that develops T1D spontaneously.63 The authors of this study demonstrated increased permeability of the small intestine (but not of the colon) in BioBreeding diabetic-prone (BBDP) rats that preceded the onset of diabetes by at least a month. Further, histological evidence of pancreatic islet destruction was absent at the time of increased permeability but was clearly present at a later time.63 Therefore, the authors presented evidence that increased permeability occurred before either histological or overt manifestation of diabetes in this animal model. Unaffected family members of probands with autoimmune diseases have also been found to have increased gut permeability.43 The BBDP rat animal model of T1D resembles this human scenario since it has been shown that "leaky gut" is part of the pathogenic equation.63 We have confirmed these data by reporting in this animal model of T1D that zonulin-dependent increases in intestinal permeability precedes the onset of T1D by 2 to 3 weeks.64 Oral administration of the zonulin inhibitor, AT1001, to BBDP rats blocked autoantibody formation and zonulin-induced loss in intestinal barrier function, reducing the incidence of diabetes.64 The involvement of zonulin in T1D pathogenesis was corroborated by our studies in humans showing that a large subgroup of T1D patients has elevated serum zonulin levels that correlated with increased intestinal permeability.65 We also provided preliminary evidence suggesting that, as in the BBDP rat model of the disease, zonulin up-regulation precedes the intestinal permeability in T1D patients.65
Therapeutic Use of the Zonulin System
Although the chronic and unregulated paracellular passage of nonself antigens can lead to allergic, inflammatory, and/or autoimmune reactions, the controlled and self-limiting modulation of intercellular TJ could represent an innovative approach for the delivery of drugs and/or molecules normally not absorbed through the GI tract.43 Considering the limitations of the TJ modulators currently tested for drug delivery,66 it was reasonable to explore whether findings from basic research on the zonulin system could be applied to developing new approaches to the enhancement of drug absorption via the regulation of intercellular TJs. Until recently, the main source of zonulin remains its biochemical purification from human cadavers. Therefore, Zot was used as a valid alternative to use the zonulin system for drug delivery, because both proteins seem to activate the same intracellular signaling leading to the modulation of intercellular TJs.
In vitro experiments in the rabbit ileum demonstrated that Zot reversibly increased intestinal absorption of insulin (MW 5733 Da) by 72% and IgG (140 to 160 kDa) by 52% in a time-dependent manner.67 In vivo31,67 and in vitro29,30 studies showed that the effect of Zot on tissue permeability occurs within 20 minutes of the addition of the protein to the intestinal mucosa and reaches a peak effect in 80 minutes. Zot was also tested in an in vivo primate model of diabetes mellitus. Insulin was intragastrically administered to diabetic monkeys either alone or in combination with increasing amounts of Zot. Measurements of blood insulin levels revealed that insulin bioavailability increased from 5.4% in controls to 10.7% and 18% when Zot 2 µg/kg and 4 µg/kg were administered.68
In the BB Wor rat model of T1D, the bioavailability of oral insulin co-administered with Zot was sufficient to lower serum glucose concentrations to levels comparable to those obtained after parenteral injection of the hormone.67 The survival time of diabetic animals chronically treated with oral insulin + Zot was comparable to that observed in parenterally treated rats. None of the animals treated with insulin and Zot experienced diarrhea, fever, or other systemic symptoms,67 providing the first in vivo evidence that the zonulin system can be exploited as an innovative strategy for the oral delivery of drugs and proteins normally not absorbed through the intestine.
Karyekar and colleagues69 have recently demonstrated that the zonulin pathway is also operative at the endothelial levels, demonstrating that Zot increases the permeability of molecular weight markers (sucrose, inulin) and chemotherapeutic agents (paclitaxel and doxorubicin) across the bovine brain microvessel endothelial cells in a reversible and concentration-dependant manner. A good correlation was established between the transport enhancement and the molecular size with r2 of 0.875. Because many therapeutic agents including anticancer drugs have molecular weights ranging from 300 to 1000 Da, these studies introduced Zot as a potential absorption enhancer for the effective delivery of therapeutic agents to the central nervous system.69
Moreover, studies have shown that Zot enhances the transport of drug candidates of varying molecular weight (mannitol, PEG4000, inulin) or low bioavailability (doxorubicin, paclitaxel, acyclovir, cyclosporin A, anticonvulsant enaminones) up to 30-fold as seen with paclitaxel across Caco-2 cell monolayers without modulating the transcellular transport.70,71
In addition, the transport-enhancing effect of Zot was reversible and nontoxic.71
Recent studies have identified a smaller 12-kDa fragment of Zot, referred to as 
G that retains Zot’s biological activity on TJs.36
In vitro studies showed that G is capable of significantly increasing the apparent permeability coefficients for a wide variety of therapeutic agents and markers across the Caco-2 cell model.72-74
In addition, G improved the bioavailability of paracellular markers, mannitol, inulin, and PEG4000 after intraduodenal administration to rats.72,73
The transport/absorption of different therapeutic agents exhibiting different physicochemical properties (lipophilicity/hydrophilicity, molecular weights, efflux properties, structural differences) and belonging to a wide range of drug classes was tested with G. For example, when the anti-HIV protease inhibitors were administered orally to Sprague Dawley rats with G, the rate and/or extent of drug absorption were significantly ameliorated, with the exception of drugs such as saquinavir, whose low oral bioavailability is mainly attributable to extensive first pass metabolism.74
The in vivo studies with G displayed up to 32-fold increases in drug bioavailability as seen with cyclosporin A after metabolic protection was provided.74
Finally, Zot is a good mucosal adjuvant, considering its ability to interfere with the suppression of specific cell-mediated immunity, probably as a result of the increased dose and/or altered processing of antigen at the mucosal level.75
To characterize further the role of Zot as an adjuvant, its ability to abrogate nasal tolerance to an unrelated protein as gliadin was examined.75
When mice were intranasally administered Zot with gliadin, the cytokine pattern showed reduced down-regulation of interleukin-2 and interferon- secretions, together with significantly less suppression in T-cell proliferation.75
This suggested that the mechanism of adjuvanticity mediated by Zot preferentially leads to a functional activation of Th1-cell differentiation.
Additional studies have shown that Zot could be exploited to deliver soluble antigens (Ag) through the nasal mucosa for the induction of Ag-specific systemic and mucosal immune responses.76,77
The co-administration of Zot and ovalbumin (Ova) was found to induce anti-Ova serum IgG (IgG) titers that were 40-fold higher than those induced by immunization with Ag alone.76
Zot also stimulated Ag-specific IgA titers in vaginal and intestinal mucosa.77
Conclusions
The GI tract has been extensively studied for its digestive and absorptive functions. A more attentive analysis of its anatomo-functional characteristics, however, clearly indicates that its functions go well beyond the handling of nutrients and electrolytes. The exquisite regional-specific anatomical arrangements of cell subtypes and the finely-regulated cross talk between epithelial, neuroendocrine, and immune cells highlights other less-studied, yet extremely important functions of the GI tract. Of particular interest is the regulation of antigen trafficking and intestinal mucosa-microbiota interactions. These functions dictate the switch from tolerance to immunity and are likely integral mechanisms involved in the pathogenesis of GI inflammatory processes.
The classical paradigm of autoimmune pathogenesis involving specific genetic makeup and exposure to environmental triggers has been challenged recently by the addition of a third element, the loss of intestinal barrier function. Genetic predisposition, miscommunication between innate and adaptive immunity, exposure to environmental triggers, and zonulin-dependent loss of intestinal barrier function secondary to a dysfunction of the intercellular TJs, all seem to be key ingredients involved in the pathogenesis of several autoimmune diseases. This new theory implies that once the autoimmune process is activated, it is not autoperpetuating. Rather it can be modulated or even reversed by preventing the continuous interplay between genes and the environment. Because TJ dysfunction allows such interactions, new therapeutic strategies aimed at re-establishing the intestinal barrier function offer innovative and hitherto unexplored approaches for the management of these devastating chronic diseases. Finally, the zonulin pathway has been exploited to facilitate delivery of drugs and antigens through several epithelial and endothelial barriers, thus allowing the passage of therapeutic agents in the GI tract, the airways, and the blood-brain-barrier.
Acknowledgements
Special thanks to Benjamin E. Weston for expertly redrawing Figure 5
.
Footnotes
Address reprint requests to Alessio Fasano, M.D., University of Maryland School of Medicine, Mucosal Biology Research Center, Health Science Facility II, Room S345, 20 Penn St., Baltimore, MD 21201. E-mail: afasano{at}mbrc.umaryland.edu
A.F. has financial interest as stock holder in Alba Therapeutics, which is currently investigating a zonulin peptide antagonist. He also serves as chair of the scientific advisory board of the company.
Accepted for publication June 30, 2008.
References
G, on the intestinal paracellular transport and oral absorption of mannitol. Int J Pharm 2003, 251:113-121[CrossRef][Medline]G on the transport and oral absorption of macromolecules. J Pharm Sci 2004, 93:1310-1319[CrossRef][Medline]G on the oral bioavailability of low bioavailable therapeutic agents. J Pharmacol Exp Ther 2005, 312:199-205
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