| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Published online before print January 15, 2009
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan
 |
Abstract |
|---|
 Top Abstract Materials and MethodsResultsDiscussionReferences |
|---|
Stem cell factor (SCF) is a critical factor for hematopoiesis and hematopoietic stem survival.5,6 It also has a role in mobilization of bone marrow stem cells, as well as in cell differentiation.7 SCF is also involved in pathogenesis of allergic airway disease and can directly induce a dose-depended increase in airway hyperreactivity8 via mast cell activation.9 Additionally, mutant mice deficient in SCF and pulmonary mast cells demonstrate significant alteration in the allergen-induced airway hyperreactive responses.8 In murine models of asthma, neutralization of SCF in vivo attenuates Th2 responses, eosinophilia, mucus production, airway remodeling, and collagen deposition.8,10-12 However, the mechanism by which SCF mediates the airway remodeling responses in asthma is not fully understood, although this may be dependent on fibrogenic factor production by SCF-responsive cells, such as eosinophils and mast cells.13-17 In one study, suppression of SCF expression using antisense oligonucleotides reduces production of interleukin (IL)-4, a well-known promoter of fibrosis.18 However a direct role for SCF in recruitment and activation of fibroblasts has not been excluded and is of particular interest in light of recent discoveries suggesting a potential role for bone marrow-derived fibroblast progenitor cells in airway remodeling and pulmonary fibrosis.4,19
Clinical evaluation of SCF in allergic asthma recently revealed another cytokine closely associated with allergic airway disease—IL-31. The authors indicated that evaluation of plasma levels of SCF and IL-31 or expression in peripheral blood mononuclear cells will be valuable parameters for the diagnosis of allergic asthma.20 IL-31 is a four-helix bundle cytokine belonging to the gp130-IL-6 family and is preferentially produced by activated T cells.21 Its activity on target cells is mediated through binding to a heterodimeric receptor composed of the IL-31 receptor A (IL-31RA) and the oncostatin M receptor (OSMR).21 In human alveolar epithelial cells transfected with IL-31RA, IL-31 activates the signal transducer and activator of transcription factor 3 (STAT-3), extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and Akt signaling pathways.22 Interleukin-31RA and OSMR are expressed in a variety of tissues including intestine, testis, skin, thymus, brain, and bone marrow.22,24,25 IL31RA deficiency exacerbates Schistosoma mansoni egg-induced lung granulomatous inflammation.23 Known target cells for IL-31 include keratinocytes, subepithelial myofibroblasts, activated monocytes, macrophages, myeloid progenitor cells, eosinophils, and bronchial epithelial cells.21,22,24-32
With regard to biological activity, IL-31 is known to induce dermal inflammation, pruritus, and severe dermatitis when overexpressed in transgenic mice.21 Bronchial epithelial cells expressed higher levels of chemokines and cytokines in response to IL-31.29 Given the recent interest on bone marrow progenitor cells in pulmonary disease, it is noteworthy that IL-31 is recently reported to significantly promote survival of both bone marrow and spleen-derived hematopoietic progenitor cells.30 Finally, and most germane to this study, elevated levels of both SCF and IL-31 proteins in peripheral blood, and their mRNAs in peripheral blood mononuclear cells have been reported in patients with asthma.33 However the precise role of IL-31 in airway remodeling (if any) remains unclear.
Thus, the present study was developed to investigate whether i) fibroblasts involved in the airway remodeling in the cockroach antigen (CRA)-induced model could originate from bone marrow; ii) the previously observed beneficial effect of anti-SCF treatment could be related to suppression of the recruitment of these bone marrow-derived cells into the sites of airway remodeling; and iii) whether these cells are regulated by IL-31. Using the chronic CRA model in GFP bone marrow chimera mice, our results showed that a significant proportion of fibroblasts in remodeling airways were derived from bone marrow. Furthermore, a significant proportion of these cells expressed c-kit, IL-31RA, and telomerase reverse transcriptase (TERT), but did not express 
-smooth muscle actin, a marker of myofibroblast differentiation. Although SCF induced IL-31RA expression in bone marrow cells, IL-31 significantly induced TERT in fibroblasts. Treatment with neutralizing antibodies to SCF caused a marked reduction in the recruitment of these cells, along with significant reduction in airway remodeling. The role of these bone marrow-derived fibroblasts might be to secrete factor(s) that promote endogenous lung myofibroblast differentiation.
|
Materials and Methods |
|---|
|
TopAbstractMaterials and MethodsResultsDiscussionReferences |
|---|
Dulbecco’s modified Eagle’s medium and antibiotics (100 U/ml penicillin, 100 µg/ml streptomycin, and 0.25 µg/ml fungizone) were from Invitrogen Corp. (Carlsbad, CA). Rat biotin-conjugated anti-mSCF R/c-kit (CD117) antibody was from R&D Systems Inc. (Minneapolis, MN). Rabbit biotin-conjugated anti-collagen type I antibody was from Rockland (Gilbertsville, PA). Mouse biotin-conjugated anti--smooth muscle actin antibody (clone 1A4) was from Lab Vision Corp. (Fremont, CA). Rabbit polyclonal anti-TERT antibody was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Mouse streptavidin-APC-Cy7, streptavidin-PE-Cy5, Fc block (clone 2.4G2), and the BD Cytofix/Cytoperm kit were from BD Biosciences (San Diego, CA).
Mice
FVB.Cg-Tg(GFPU)5Nagy or FVB/NJ mice were purchased from The Jackson Laboratory (Bar Harbor, ME) and maintained under specific pathogen-free conditions. We obtained approval for experimental protocols and mouse use from The University of Michigan Committee on Use and Care of Animals.
Bone Marrow Chimera Mice
Bone marrow chimeras were prepared as previously described.19 Bone marrow cells were collected from femurs and tibias of donor GFP Tg or wild-type FVB mice by aspiration and flushing. Recipient FVB mice were exposed to two doses of 5 Gy given 3 hours apart using a 137Cs irradiator, and then maintained on acidified water and autoclaved feed ad libitum. After irradiation, 4 x 106 bone marrow cells from GFP FVB mice in a volume of 200 µl of sterile phosphate-buffered saline (PBS) were injected retro-orbitally under anesthesia.
CRA Challenge
Five weeks after bone marrow transfection mice were sensitized intraperitoneally and subcutaneously with 1000 protein nitrogen units (PNUs) of cockroach Ag (CRA; Holliser Stier, Toronto, Canada) 1/1 in incomplete Freunds adjuvant (Sigma-Aldrich, St. Louis, MO) as we described before.10-12,14,15,34-39
Then mice were challenged intranasally with 150 PNUs of CRA every 4th day after initial sensitization to localize the response to the lung (Figure 1)
. The final two allergen challenges (500 PNUs) were given 4 days apart by intratracheal injection. Then animals were euthanized and lungs were removed. In some experiments along with the intratracheal injections of cockroach allergen, mice were given either anti-SCF (50 µg, rabbit anti-murine SCF Abs) or control antibody treatment (50 µg, total IgG).
|
Rabbit anti-murine SCF Abs was prepared by multiple-site immunization of New Zealand White rabbits with recombinant murine SCF (R&D Systems Inc., Minneapolis, MN) in CFA. Polyclonal Abs were titered by direct enzyme-linked immunosorbent assay and specifically verified by the failure to cross-react to mIL-3, mIL-1β, mTNF-, mMIP-1, IL-6, mJE, mMIP-1β, mMCP-1, mIL-8, mRANTES, mMIP-1, mTNF-β, and mMIP-1. The IgG portion of the serum was purified over a protein A column.
Assaying of SCF Level in Plasma
Anesthetized animals were bled by aortic puncture into titrated plastic syringes (50 µl of 0.2 mol/L Tris-citrate/ml of blood). The syringes were placed in ice and all subsequent manipulations (except as stated below) were done in plasticware at 4°C. Samples were centrifuged for 18 minutes at 850 x g and the supernatant recentrifuged at 26,800 x g for 30 minutes. The resulting platelet-depleted plasma was transferred into glass tubes and clotted for 1 hour at 56°C in a presence of 5 mmol/L of CaCl2. Then the samples were centrifuged at 60,000 x g for 2 hours and the resulting plasma was used to determine SCF levels. SCF capturing and detection antibodies were from R&D Systems Inc.
Quantitative Polymerase Chain Reaction (PCR) Analysis
RNA was isolated using Trizol reagent (Invitrogen) and each sample was reverse-transcribed into cDNA. QPCR analysis was performed using TaqMan reagents and predeveloped reagent kits (PE Biosystems, Foster City, CA) on 2 µl of cDNA in a total reaction volume of 25 µl.
Morphological Fluorescence Analysis
The lungs were fully inflated by the intratracheal perfusion with 4% paraformaldehyde. Lungs were then dissected and placed in fresh paraformaldehyde on ice for 6 hours, infused with graded sucrose washes for 24 hours, and finally fixed and frozen in OCT compound (Miles Inc., Elkhart, IN). Serial cryostat sections (4 µm thick) were then analyzed by fluorescence microscopy within 1 hour to evaluate distribution or localization of GFP+ cells. Some tissue sections were stained to visualize collagen I, GFP, and -smooth muscle actin. Tissue sections were washed in PBS, blocked with Fc block, 1:200 in DPBS plus 7% bovine serum albumin plus 1% fetal calf serum plus 1% normal goat serum and permeabilized with 0.25% Triton X-100 in DPBS for 5 minutes at room temperature in the dark. Then specimens were stained with i) anti-collagen I primary antibodies (AbCam, ab34710) and goat anti-rabbit IgG Alexa Fluor-568-conjugated secondary antibodies (Molecular Probes/ Invitrogen), 1:400 (all in PBS plus 2% bovine serum albumin plus 0.05% sodium azide plus 2% normal goat serum); ii) anti--smooth muscle actin fluorescein isothiocyanate-conjugated antibodies; iii) anti-GFP Alexa Fluor-647-conjugated antibodies (Molecular Probes/Invitrogen). Images were taken using a confocal microscope (Zeiss, Thornwood, NY).
Whole Lung Histological Analysis
The lungs were fully inflated by the intratracheal perfusion with 4% paraformaldehyde. Lungs were then dissected and placed in fresh paraformaldehyde for 24 hours. Routine histological techniques were used to paraffin-embed this tissue, and 4-µm sections of whole lung were stained with Masson Trichrome or hematoxylin and eosin. Inflammatory infiltrates and other histological changes were examined around bronchioles and larger airways, using light microscopy.
Flow Cytometry
Whole lungs from mice were dispersed using type IV collagenase; red blood cells were then lysed (using RBC lysis buffer: 388 mmol/L NH4Cl, 29.7 mmol/L NaHCO3, 25 µmol/L Na2EDTA) for 3 minutes on ice, and total number of cells per lung counted. After appropriate washing and blocking with Fc block (CD16/32), 1 x 106 cells were surface-stained with biotin-conjugated c-kit antibody. Secondary antibodies for c-kit were streptavidin-PE-Cy5-conjugated. Then cells were fixed and permeabilized using the BD Cytofix/Cytoperm kit and stained with rabbit biotin-conjugated anti-collagen I or isotype-matched control IgG. The staining was detected with streptavidin-APC-Cy7-conjugated antibody. For some experiments cells also were stained with rabbit anti-TERT antibody and adequate PE-conjugated secondary detection antibody or PE-Cy7-conjugated CD11b antibody. Flow-cytometric analysis was undertaken using a BD LSR II and flow sorting was done using FACS ARIA (both BD Biosciences). Data collected were analyzed using FlowJo software (Tree Star, Inc., Ashland, OR).
Mouse Lung Fibroblast Culture
Mouse lung fibroblasts were isolated from lung tissue by mincing and enzymatic digestion with collagenase IV as previously described.19 After filtration, released cells were washed and then used for sorting. After flow-sorting, GFP-positive and CD11b-negative cells were cultured for 12 hours to obtain conditioned media (culture media: STEMPROR MSC SFM serum-free medium for human mesenchymal stem cells; Invitrogen). Control aged media were also placed in empty (without cells) wells under similar incubation conditions. Where indicated, control aged media alone or conditioned media from GFP+ fibroblasts were mixed 50:50 with control aged media and used to treat GFP– fibroblasts. Samples were collected after 6 hours in culture.
Analysis of Cultured Cells by Flow Cytometry
Cells were washed and detached on ice using Versene mix. Cell staining was performed as described for whole lung mince.
Statistics
Statistical significance was determined using analysis of variance with P values less than 0.05 followed by Student-Neuman-Keuls posttest when appropriate.
|
Results |
|---|
|
TopAbstractMaterials and MethodsResultsDiscussionReferences |
|---|
GFP bone marrow chimera mice have been used previously to document recruitment of bone marrow-derived fibroblasts to the lung in response to lung injury and fibrosis.4,19
Based on previous studies and the ready availability of GFP transgenic mice in the FVB/NJ background, initial experiments used this background strain for donor and recipient mice. After stable engraftment, the animals were chronically challenged with CRA (Figure 1)
to induce an airway remodeling as we described before.10-12,14,15,34-39
The presence of GFP-positive and collagen-positive cells in the tissue, as well as -smooth muscle actin-positive but GFP-negative cells was illustrated by immunofluorescence (Figure 2A)
. The representative lung tissue section from a chronic CRA-treated animal was immunostained for GFP (green), collagen I (red), and -smooth muscle actin (blue). The GFP-positive and collagen I-positive cells appear yellowish-green and are indicated by arrows, whereas the -smooth muscle actin-positive and collagen I-positive, but GFP-negative cells appear purplish and are indicated by arrowheads.
|
. A significant number of GFP-positive and collagen I-producing cells also expressed c-kit (Figure 2C)
.
TERT expression is induced in lung injury and fibrosis, and associated with some of the bone marrow-derived fibroblasts.13,40-44
Figure 3A
showed induction of TERT in CRA-challenged lung-derived cells, with most (>70%) being derived from bone marrow. Comparable numbers of bone marrow-derived cells were c-kit-positive (Figure 3B)
. In contrast, virtually all of the bone marrow-derived fibroblasts did not express -smooth muscle actin, which was expressed only in the GFP-negative population (Figure 3C)
. Thus a significant proportion of fibroblasts in the CRA-challenged lung were derived from bone marrow precursors, many of which still expressed progenitor markers but not -smooth muscle actin, a marker of myofibroblast differentiation.
|
To more definitively analyze the phenotype of the recruited bone marrow-derived lung fibroblasts, whole lung digests were purified for GFP-positive and CD11b-negative cells by flow cytometry. Normal wild-type lung digests were used as a GFP-negative control (Figure 4A)
to set up the appropriate sorting gates as shown in Figure 4, B and C
. To avoid macrophages, the GFP-positive and CD11b-negative population was flow-sorted (Figure 4C)
. Most of the sorted GFP-positive and GFP-negative cells have typical fibroblast-like morphology (Figure 4D)
. For further analysis of phenotype, mRNA from these cells was analyzed for several marker gene expressions. The results (Figure 4E)
showed that both GFP-positive and GFP-negative cell cultures expressed vimentin, an intermediate intracytoplasmic filament expressed in fibroblasts.45
Expression of collagen I, although readily detectable in GFP-positive cells, was significantly lower than that in GFP-negative cells, consistent with the flow cytometric analysis (Figure 2)
. Expression of -smooth muscle actin was readily detectable in GFP-negative cells, but was dramatically lower in GFP-positive cells, where it was close to the limit of detection. Interestingly GFP-negative fibroblasts also expressed tenascin C (Figure 4E)
, which is expressed in fibroblast foci, areas consisting of fibroblasts and myofibroblasts found in lung biopsies of patients with idiopathic pulmonary fibrosis.46
Tenascin C is an oligomeric extracellular matrix glycoprotein suggested to play a structural role and to modulate the adhesive and migratory functions of cells. In contrast, bone marrow-derived fibroblasts expressed much lower and barely detectable levels of tenascin C (Figure 4E)
. Freshly isolated murine eosinophil mRNA was used as a negative control for collagen I, -smooth muscle actin, vimentin, and tenascin C expression, and did not exhibit any amplification products for these genes. These findings confirmed that although bone marrow-derived fibroblasts were recruited to the lung in this CRA model, they were phenotypically distinct from fibroblasts that were of local or pulmonary origin.
|
Previous studies have shown the importance of SCF in the pathogenesis of murine models of asthma,6,9-14,47
but the precise role(s) of SCF remains incompletely understood. In view of evidence in the current study showing bone marrow origin of c-kit-expressing fibroblasts in remodeling airways of mice exposed to chronic CRA challenge, the possibility that SCF might be important in their recruitment was examined. Whole lung mince from naïve mice and mice challenged with CRA without or with treatment with neutralizing antibody to SCF, were digested to obtain single cell suspensions for flow cytometric analysis. The results showed a significant increase (2.7-fold) in GFP and collagen I-positive cells after chronic CRA challenge (Figure 5A)
relative to that in unchallenged animals. Anti-SCF antibody treatment given with the two final intratracheal CRA challenges caused significant reduction in the number of cells expressing both GFP and collagen I, to essentially control levels (3.2-fold decrease, CRA + aSCF over CRA) (Figure 5A)
. Additionally, 21.4% of GFP- and collagen I-positive cells from chronic CRA animals were also c-kit-positive (Figure 5B)
and this population was also reduced to control levels on treatment with anti-SCF antibodies. The total population of GFP-positive and c-kit-positive cells were also elevated (2.98-fold increase over controls) after chronic CRA treatment (Figure 5C)
. Neutralization of SCF caused significant reduction in this (1.49-fold decrease) GFP and c-kit-positive population, but not completely to control levels as was observed with bone marrow-derived collagen-producing cells. Thus, neutralization of SCF in vivo caused a significant reduction in the recruitment of bone marrow-derived fibroblast progenitor cells to the remodeling airway.
|
Neutralization of SCF significantly reduced peribronchial remodeling and collagen deposition in the CRA model of asthma.10
To extend this observation to remodeling in the GFP bone chimera mice and assess the role of bone marrow-derived cells, anti-SCF antibodies were administered with the two final allergen challenges as diagrammed in Figure 6A
. Neutralization of SCF significantly reduced the number of CRA-induced GFP-positive cells in the lung back to essentially control levels (Figure 6B)
. To confirm the flow cytometric analysis, and the associated impact on airway remodeling, GFP bone marrow chimera mice were chronically challenged with cockroach allergen to develop asthma and the effects of SCF neutralization examined by immunohistopathology. The results showed that although control lungs exhibited normal architecture with occasional GFP-positive cells (Figure 6C
, Con panel), CRA-challenged lungs showed marked infiltration by GFP-positive cells (Figure 6C
, CRA panels). In tissue sections from CRA-challenged mice treated with anti-SCF antibodies, there was a marked reduction in the number of GFP-positive cells (Figure 6C
, CRA + SCF panel) with only a scattering of mostly mononuclear cells in the interstitial areas. These findings were consistent with the flow cytometric analysis showing the marked reduction in GFP-positive cell recruitment by the anti-SCF treatment. Moreover, when the tissue sections were analyzed for extent of remodeling and presence of myofibroblasts, the efficacy of anti-SCF was also confirmed (Figure 6D)
. Masson Trichrome staining of the lung tissue sections for evaluation of collagen deposition revealed significant fibrosis of the airway by CRA challenge in these FVB mice, which was markedly reduced by anti-SCF treatment. Significant numbers of myofibroblasts were also detected by immunostaining for -smooth muscle actin, which were also reduced by the anti-SCF treatment. Reduction in mucus plugging also accompanied the reduction in airway remodeling. These findings were comparable to those previously studied using Balb/C mice.10-12
Thus neutralization of SCF resulted in diminished recruitment of bone marrow-derived cells, including fibroblasts, which correlated with reduction in airway remodeling. We also assayed plasma level of SCF in chronic allergen-challenged and anti-SCF antibody-treated animals. We found that neutralization of SCF significantly suppressed CRA-induced SCF in plasma (Figure 7)
. The neutralization of SCF also had an interesting effect on lung-derived fibroblasts, cultured as we described before. Lung fibroblasts derived from chronic allergen-challenged animals displayed significantly up-regulated TERT expression (Figure 8)
. Whereas in fibroblasts derived from lungs of anti-SCF-treated animals TERT expressions were significantly suppressed. As we already mentioned before, TERT expression is induced in lung injury and fibrosis, and associated with some of the bone marrow-derived fibroblasts.13,40-44
|
|
|
-smooth muscle actin and procollagen I gene expression (Figure 9, A and B)
. Thus, although the bone marrow-derived (GFP-positive) fibroblasts themselves did not undergo myofibroblast differentiation, they did secrete factor(s) that could promote myofibroblast differentiation in endogenous lung fibroblasts (GFP-negative). Transforming growth factor (TGF)-β was highly expressed in the bone marrow-derived fibroblasts (data not shown), and thus could represent the key factor in the conditioned media responsible for the promotion of endogenous lung myofibroblast differentiation. To address the role of TGF-β in that experiment, we used anti-TGF-β1, -2, and -3 together with conditioned media. Neutralization of TGF-β1, -2, and -3 partially inhibited conditioned media-induced collagen I expression (Figure 9C)
.
|
Allergic airway disease is characterized by elevated lung and blood IL-31 expression.21,33
We confirmed that lung IL-31 expression was significantly elevated in the CRA model 6 hours after allergen challenge (Figure 10A)
. Given that hematopoietic progenitor cells express IL-31 RA and are able to respond to IL-31 with increased survival,30
the possibility that bone marrow-derived fibroblasts could express this receptor and also respond to IL-31 was examined by real-time PCR. Initial evaluation of lung cell IL-31RA expression by flow cytometry revealed a significant increase (more than fourfold) in the number of cells expressing this receptor in antigen-challenged mice relative to that in control mice (Figure 10B)
. When this was combined with analysis for collagen I expression as a marker for fibroblasts, the results showed a comparable increase in the number of fibroblasts expressing IL-31RA in the antigen-challenged murine samples. To confirm that expression was in fibroblasts and to distinguish between bone marrow-derived versus lung endogenous fibroblasts, the fibroblast population from CRA-challenged GFP bone marrow chimeric mice were isolated and purified by removal of CD11b-positive cells by magnetic bead separation. These CD11b-negative fibroblasts were then flow sorted into GFP-positive (bone marrow-derived) and GFP-negative (endogenous lung origin) populations and analyzed for IL-31RA expression by real time PCR. The results showed a 140-fold higher level of IL-31RA expression in GFP-positive fibroblasts relative to that in GFP-negative fibroblasts, indicating that virtually all of the fibroblast IL-31RA expression in CRA-challenged lungs was from bone marrow-derived fibroblasts (Figure 10C)
. OSMR, the heterodimeric partner of IL-31RA was also expressed in the GFP-positive-CD11b-negative fibroblasts (data not shown), thus suggesting their myeloid progenitor origin. Indeed, isolated bone marrow cells expressed IL-31RA, which was markedly enhanced (more than fourfold) by SCF treatment (Figure 10D)
. Finally IL-31 was found to be a potent inducer of TERT expression in lung fibroblasts expressing IL-31RA (Figure 10E)
, which was not suppressed by TGF-β (data not shown) a known inhibitor of TERT expression.48
Thus, stimulation of lung fibroblasts in vitro with IL-31 leads to increased TERT expression, which is associated with increased survival of lung fibroblasts and favors the development of fibrosis instead of injury resolution.48
Taken together the data indicated the importance of bone marrow-derived c-kit+collagenI+IL-31RA+CD11b– cells that could provide an interesting link between SCF and IL-31 roles in allergic airway disease.
|
|
Discussion |
|---|
|
TopAbstractMaterials and MethodsResultsDiscussionReferences |
|---|
Previously GFP bone marrow chimera mice have been used to show significant contribution of bone marrow progenitors to the lung fibroblast population in animal models of pulmonary fibrosis.3
Based on the data shown here, this observation can be extended to recruitment of fibroblasts to the remodeling airway. Significant numbers of GFP-positive (indicative of bone marrow origin) fibroblasts were seen in the CRA-challenged lung and could be isolated and analyzed in vitro. These bone marrow-derived fibroblasts did not express -smooth muscle actin indicating that myofibroblasts present in the remodeling airway in CRA-challenged mice were not derived from bone marrow progenitors. However a significant proportion (>20%) of these bone marrow-derived fibroblasts expressed markers that are more closely associated with stem cells, namely c-kit and TERT, indicating perhaps their relative immaturity or less differentiated state. Supporting this possibility was the finding that these cells expressed high levels of IL-31RA, which is expressed by hematopoietic progenitor cells.30
This population might represent more recently migrated or recruited progenitor cells that had not differentiated to assume the mature fibroblast phenotype. Expression of these two markers was not restricted to bone marrow-derived cells, suggesting some contribution by potential progenitor cells of intrapulmonary origin. Recruitment of c-kit-positive cells to sites of tissue injury is not unprecedented, and has been recently reported in myocardial injury.57
In view of expression of these markers by the bone marrow-derived cells, especially c-kit, the possibility that SCF may play a role in their recruitment was examined. The findings confirmed the efficacy of neutralizing anti-SCF antibodies to suppress airway remodeling, but more interestingly they also inhibited recruitment of the bone marrow-derived fibroblasts, including those that expressed c-kit. The ability of SCF to recruit stem cells is well documented, perhaps because of its chemotactic properties and ability to promote survival of these cells.5,58,59 Interestingly stem cells with long-term engraftment capabilities can be mobilized by s-kit (soluble c-kit), and that s-kit combined with G-CSF treatment leads to significant enhancement of engraftment efficiency, suggesting mobilization via disruption of c-kit and SCF interaction as the mechanism.7 The bone marrow progenitor cell population that gave rise to the lung fibroblast in this CRA model remains to be identified. An additional role for SCF is suggested by the finding that it could up-regulate expression of IL-31RA, although the importance of IL-31 in airway disease and remodeling requires further clarification. A significantly elevated level of IL-31 in plasma of allergic asthma patients was recently documented.20 It may potentially be a key factor in the induction of TERT in the bone marrow-derived fibroblasts, which previously has been shown to promote survival of these cells.49 This could result in the promotion of fibrosis by prolonged or enhanced production of fibrogenic factors from these cells to enhance myofibroblast differentiation. Further studies are necessary to further elucidate the role of IL-31, but the findings from this study reveal a potential novel link between SCF and IL-31 in the regulation of bone marrow-derived fibroblast recruitment and function that are germane to the pathogenesis of allergic airway disease.
Studies relying on immunostaining for fibrocyte markers suggest that these circulating cells are a source of myofibroblasts in airway remodeling.3,4
Because these cells are assumed to be derived from bone marrow progenitors, the findings in this study using the GFP bone marrow chimera mice appear to be at variance with the implication of the fibrocyte studies. This discrepancy is also noted in other studies using these two different approaches of monitoring extrapulmonary origin of fibroblasts in response to lung injury.2,46,60
Using similar bone marrow chimera mice, the origin of myofibroblasts from bone marrow progenitors could not be demonstrated in wound healing and a model of liver fibrosis, despite the presence of bone marrow-derived collagen I-producing cells.61,62
In another study, transgenic mice expressing GFP under control of the -smooth muscle actin promoter are used as bone marrow donors for transplantation into wild-type mice, creating a bone marrow chimera that only has this transgene in the bone marrow cells.63
Using these chimera mice, the study reveals that the bone marrow is not a source for -smooth muscle actin expressing cells in distal organs. In vitro culture of bone marrow cells however can yield -smooth muscle actin-expressing cells, but on transplantation, they apparently cannot give rise to myofibroblasts or smooth muscle cells in distal organs in vivo. The possibility that fibrocytes may originate from tissues (eg, endothelium) other than the bone marrow has not been excluded. In this regard, it is noteworthy that endothelial cells have been reported to have the capacity to transition to an -smooth muscle-expressing cell phenotype.64
In summary, the studies reported herein revealed significant recruitment of bone marrow-derived fibroblasts to remodeling airway in the CRA model characterized by induced expression of SCF. The recruited cells did not express -smooth muscle actin, a marker of myofibroblast differentiation. However a significant subpopulation of these recruited cells expressed markers that are more characteristic of progenitor cells, namely c-kit, IL-31RA, and TERT. Neutralization of SCF resulted in reduced remodeling and a marked reduction in recruitment of these bone marrow-derived cells. Taken together these findings for the first time revealed a novel role for SCF, perhaps in conjunction with IL-31, in airway remodeling that is related to its involvement in the recruitment of bone marrow-derived fibroblasts with the capacity to promote myofibroblast differentiation in endogenous lung fibroblasts.
|
Footnotes |
|---|
Supported by the National Institutes of Health (grants HL28737, HL31963, HL52285, and HL77297 to S.H.P. and HL59178 to N.W.L.) and the Sandler Program in Asthma Research (award to S.H.P.).
Accepted for publication November 4, 2008.
|
References |
|---|
|
TopAbstractMaterials and MethodsResultsDiscussionReferences |
|---|
Related Article
This article has been cited by other articles:
 |
|
 |
|
  J. D. Miller, S. M. Lankford, K. B. Adler, and A. R. Brody Mesenchymal Stem Cells Require MARCKS Protein for Directed Chemotaxis In Vitro Am. J. Respir. Cell Mol. Biol., September 1, 2010; 43(3): 253 - 258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Murphy and P. M. O'Byrne Recent Advances in the Pathophysiology of Asthma Chest, June 1, 2010; 137(6): 1417 - 1426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Brody, K. D. Salazar, and S. M. Lankford Mesenchymal Stem Cells Modulate Lung Injury Proceedings of the ATS, May 1, 2010; 7(2): 130 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Frid, M. Li, M. Gnanasekharan, D. L. Burke, M. Fragoso, D. Strassheim, J. L. Sylman, and K. R. Stenmark Sustained hypoxia leads to the emergence of cells with enhanced growth, migratory, and promitogenic potentials within the distal pulmonary artery wall Am J Physiol Lung Cell Mol Physiol, December 1, 2009; 297(6): L1059 - L1072. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Dolgachev, B. C. Petersen, A. L. Budelsky, A. A. Berlin, and N. W. Lukacs Pulmonary IL-17E (IL-25) Production and IL-17RB+ Myeloid Cell-Derived Th2 Cytokine Production Are Dependent upon Stem Cell Factor-Induced Responses during Chronic Allergic Pulmonary Disease J. Immunol., November 1, 2009; 183(9): 5705 - 5715. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
