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Published online before print April 6, 2009
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and Vascular Endothelial Growth FactorFrom the Oregon Hearing Research Center, Department of Otolaryngology and Head and Neck Surgery, Oregon Health and Science University, Portland, Oregon
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Abstract |
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 Top Abstract IntroductionMaterials and MethodsResultsDiscussionReferences |
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and the up-regulation of vascular endothelial growth factor (VEGF). Inhibition of HIF-1 activity caused a decrease in VEGF expression levels in stria vascularis vessels. Blockade of VEGF activity with SU1498, a VEGF receptor inhibitor, significantly attenuated the expression of desmin in pericytes. These data demonstrate that cochlear pericytes are markedly affected by acoustic trauma and display an abnormal morphology. HIF-1 activation and VEGF up-regulation are important factors for the alteration of the pericyte structural protein desmin. |
Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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The cochlear microvasculature network has a high population of pericytes.10
Pericytes form a dense net over the capillaries of the stria vascularis. However, unlike pericytes in the capillaries of the cochlear spiral ligament or other organs, pericytes on the stria vascularis do not express contractile proteins such as -SMA or tropomyosin but they are rich in the structural protein desmin.10
Pericytes, in general, are thought to give mechanical strength and enhance the general integrity of capillary networks, as a case in point, blood vessels deficient of pericytes are abnormally large and leaky.11
The pericytes are known to have important roles in the regulation of vascular development, stabilization, maturation, and remodeling.12-20 Pericytes also mediate physiological repair processes including adaptive responses that protect vulnerable tissues under stress.21-25 Proliferation and recruitment of pericytes have frequently been found in ischemic tissues damaged by stroke.25,26 They are also morphologically altered in ischemia and under other hypoxic conditions.26,27
The hypoxic environment induced in the cochlea by noise is immediate and the effects persist after the noise is terminated.6,28
Studies have shown that when cells encounter low oxygen tension, they adapt by promoting expression of genes associated with anaerobic cell metabolism, cell survival, and angiogenesis.29
This transcriptional response is mediated by a hypoxia-inducible factor (HIF),30,31
a principal transcription factor, involved in the regulation of transcriptional responses to hypoxia. HIF is a heterodimer comprised of and β subunits, which are constitutively expressed.32,33
Expression of the β subunit is independent of oxygen, whereas the protein stability of the subunit is regulated in accordance with cellular O2 levels.33
HIF-1 subunits are degraded in normoxia, but are stabilized and activated under hypoxic conditions, forming a complex with the constitutively expressed transcription factor ARNT/HIF1β, which increases the transcription of target genes. Target genes of Hif-1 including the gene of vascular endothelial growth factor (Vegf) are related to angiogenesis, cell proliferation, and survival.30,34
The biological activity of vascular endothelial growth factor (VEGF) protein has been studied extensively in vascular remodeling under the ischemia condition.35,36
In particular, emerging evidence shows that VEGF has important roles in pericyte proliferation and in turn, pericytes are critical in vascular remodeling.35-37
In this study, we investigated cochlear pericyte change in response to noise-induced hypoxia and involvement of HIF-1 and VEGF in the change. Our results show that high-level noise exposure increased the expression of pericyte structural protein desmin and that excess numbers of pericytes decorate vessels resulting in increased coverage over the vessels. HIF-1 induction and VEGF up-regulation are found to be influenced by loud sound and they are important components for altering pericyte structure in response to noise stimuli.
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Materials and Methods |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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Experiments were performed on albino guinea pigs (both sexes; weight, 300 to 450 g) and 129S2/C57BL/6 mice (8 to 10 weeks old). All procedures in this study were reviewed and approved by the Institutional Animal Care and Use Committee at Oregon Health and Science University.
Noise Exposure
The animals (guinea pigs and mice) with positive Preyer reflex were divided into control and noise-exposed groups. For noise exposure, the animals were placed in wire mesh cages and exposed to broadband noise at 120 dB SPL in a sound exposure booth for 30 minutes, 1 hour, 3 hours, or for an additional 3 hours the next day. This noise exposure regime is routinely used in our laboratory and produces a permanent cochlear sensitivity loss.38
Auditory Testing
Auditory brain-stem response audiometry to pure tones was used to evaluate hearing function before noise exposure and after noise exposure in five mice and five guinea pigs. For the auditory brain-stem response test, each animal was anesthetized with xylazine (10 mg/kg, i.m., IVX; Animal Health Inc., St. Joseph, MO) and ketamine (40 mg/kg, i.m.; Hospira, Inc., Lake Forest, IL), and placed on a heating pad in a sound-isolated chamber. The external ear canal and tympanic membrane were inspected using an operating microscope to ensure the ear canal was free of wax and that there was no canal deformity, no inflammation of the tympanic membrane, and no effusion in the middle ear. Needle electrodes were placed subcutaneously near the test ear, at the vertex and at the contralateral ear. Each ear was stimulated separately with a closed tube sound delivery system sealed into the ear canal. The auditory brain-stem response to a 1-ms rise-time tone burst at 4, 8, 12, 16, 24, and 32 kHz was recorded and thresholds obtained for each ear. Threshold was defined as an evoked response of 0.2 µV. This method was used to assess auditory brain-stem response both before noise exposure and immediately after noise exposure.
Immunohistochemistry
Primary antibodies used in the experiments included monoclonal rabbit anti-desmin (catalog no. ab32362; Abcam, Cambridge, MA), polyclonal rabbit anti-VEGF (catalog no. sc-507; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), polyclonal rabbit anti-HIF-1 (catalog no. sc-10790, Santa Cruz Biotechnology, Inc.), Alexa Fluor 568-conjugated goat anti-mouse IgG (H+L) (catalog no. A11001; Invitrogen, Eugene, OR), Alexa Fluor 568-conjugated goat anti-guinea pig IgG (H+L) (catalog no. A11073, Invitrogen). Secondary antibodies were Alexa Fluor 488-conjugated goat anti-rabbit (catalog no.11008, Invitrogen, Eugene, OR). Immunohistochemistry was performed as described previously.38
Tissue sections were briefly permeabilized in 0.5% Triton X-100 (Sigma, St. Louis, MO) for 1 hour, and immunoblocked with a solution of 10% goat serum and 1% bovine albumin in 0.02 mol/L phosphate-buffered saline (PBS) for 1 hour. The specimens were incubated overnight at 4°C with the primary antibody diluted in PBS-bovine serum albumin. After several washes in PBS, sections were incubated in secondary antibody for 1 hour at room temperature. Tissues were mounted in mounting medium (H-1000; Vector Laboratories, Inc., Burlingame, CA) and visualized under an Eclipse TE 300 inverted microscope (Nikon, Tokyo, Japan) fitted with a Bio-Rad (Richmond, CA) MRC 1024 confocal laser microscope system. Controls were prepared by replacing primary antibodies with 0.2% Triton X-100 in PBS.
Western Blot Analyses
Desmin expression under control and noise-stimulated conditions was compared. Guinea pigs and mice from control and on the second day of noise-exposed groups were anesthetized with an overdose of ketamine hydrochloride (100 mg/kg, i.m.) and 2% xylazine hydrochloride (10 mg/kg) (Abbott Laboratories, N. Chicago, IL). The cochleae were taken after cardiovascular perfusion with PBS (pH 7.4). Each guinea pig group was comprised of six animals; each mouse group was comprised of 10 animals. The guinea pig and mouse cochleae were removed and the whole cochlear lateral walls of the guinea pigs were immediately dissected in ice-cold PBS. For the mouse cochlea, the cochlear stria vascularis was isolated from the cochlear lateral wall in cold PBS. Tissue samples were washed twice with cold PBS and immediately frozen at –80°C in 1.5-ml Eppendorf tubes. Total protein was extracted following the manufacturer’s instructions (catalog no. 20-188; Upstate, Lake Placid, NY). The protein was removed and stored at –20°C. The protein concentrations were calculated using a bicinchoninic acid protein assay kit (Pierce, Rockford, IL). Equal amounts of protein were loaded on 12% sodium dodecyl sulfate-polyacrylamide gels (90 V, room temperature for 80 minutes), electrophoresed, and transferred to nitrocellulose by electroblotting (30 V, overnight at 4°C) in 1x transfer buffer (Bio-Rad). Nitrocellulose membranes were blocked in 5% w/v nonfat dry milk and 0.1% v/v Tween 20 in PBS (pH 7.4, 0.12 mol/L) for 1 hour at 25°C before being incubated overnight at 4°C with primary antibodies [monoclonal rabbit anti-desmin 1:500 (catalog no. ab32362, Abcam, Cambridge, MA), polyclonal rabbit anti-VEGF 1:500 (catalog no. sc-507, Santa Cruz Biotechnology, Inc.)] in blocking buffer. Membranes were then incubated with a 1:3000 v/v secondary antibody (Bio-Rad) for 1 hour at room temperature. Protein bands were visualized on Kodak XAR-5 film (Eastman-Kodak, Rochester, NY) with Super Signal West Femto chemiluminescent substrate (Pierce, Rockford, IL). Equal protein loading was examined by stripping the blots and reprobing them with a 1:5000 v/v of monoclonal mouse antibody for GAPDH (Chemicon International, Temecula, CA) or monoclonal mouse antibody for -actin (1:3000, catalog no. A1978; Sigma) followed by incubation with secondary antibody (catalog no. 170-6516, goat anti-mouse antibody; Bio-Rad) and visualization as described above.
Quantitative Real-Time Polymerase Chain Reaction (PCR)
Total RNA from the cochlear lateral wall was separately extracted, with RNeasy (Qiagen, Valencia, CA), from the control, noise-exposed, and drug-pretreated noise-exposed groups. Each group was comprised of 10 mice for analysis of mRNA levels of Desmin and Vegf with quantitative real-time PCR. Two µg of total RNA and 100 ng of random hexamer were used to make 40 µl of cDNA by SuperScript II (Invitrogen) following the manufacturer’s instructions. Transcript quantities were assayed by corresponding TaqMan gene expression assay: Desmin (catalog no. Mm00802455_m1; Applied Biosystems, Foster City, CA) and Vegf (catalog no. Rh02621759_m, Applied Biosystems) with a model 7300 real-time PCR system (Applied Biosystems, Foster City, CA). Thermal cycle conditions were an initial hold of 50°C for 2 minutes, 95°C for 10 minutes, then 40 cycles of 95°C for 15 seconds and 60°C for 1 minute. For assessment of mRNA level of Hif-1, total RNA from the cochlear lateral wall was separately extracted from the control, noise exposure (30 minutes), noise exposure (1 hour), and noise exposure 3 hours in each day for 2-day groups. Each group was comprised of three mice. Two µg of total RNA sample was reverse-transcribed by using RETROscript kit (Ambion, Austin, TX). The cDNA synthesized from total RNA was diluted 10-fold with DNase-free water. Transcript quantities were assayed by corresponding TaqMan gene expression assay Hif-1 (catalog no. Mm01283758_g1, Applied Biosystems) with a StepOnePlus real-time PCR system (Applied Biosystems). Cycling conditions of real-time PCR were 95°C for 20 seconds, 40 cycles of 95°C for 1 second, 60°C for 20 seconds. Mouse Gapdh (catalog no. 4352339E, Applied Biosystems) expression was used as an endogenous control. Samples were run in triplicate for Desmin and Vegf. Samples were run six times for Hif-1. Quantitative PCR was performed according to the guidelines provided by Applied Biosystems. The comparative cycle threshold (CT) method (
CT quantitation) was used to calculate the difference between samples. Quantitative data analysis followed the suggestions of the manufacturer.
Transmission Electron Microscopy
Cochlear lateral wall tissues were dissected from the control and noise-exposed animals. Segments of the cochlear lateral wall from the basal turns were fixed overnight in phosphate-buffered 3% glutaraldehyde-1.5% paraformaldehyde and postfixed in 1% osmium. Tissues were dehydrated, embedded in Araldite plastic, sectioned, stained with lead citrate and uranyl acetate, and viewed in a Phillips, CM, 100 transmission electron microscope (Eindhoven, the Netherlands).
Double and Triple Labeling
To visualize pericytes on the endothelium of the cochlear lateral wall, we double labeled lateral wall tissues with a combination of antibody for desmin (to identify pericytes) and isolectin GS-IB4 Alexa Fluor 488 (to identify vessels) (catalog no. 121411, Invitrogen). The procedure after immunohistochemical labeling for desmin was the same as described above except that 1:400 isolectin GS-IB4 was added to the medium along with primary antibody for desmin.
To visualize HIF-1 translocation, we triple labeled lateral wall tissues with a combination of antibody for HIF-1, propidium iodide (catalog no. P-3566, Molecular Probes, Eugene, OR) (to identify cell nuclei) and isolection GS-IB4 Alexa Fluor 647 (to identify vessels, catalog no. I32450, Invitrogen). The procedure after immunohistochemical labeling for HIF-1 was the same as described above except that 1:100 propidium iodide was added to the medium along with secondary antibody for HIF-1 for 1 hour.
Desmin Coverage on Vessels
Images were analyzed using Image J (V1.38X; National Institutes of Health, West Chester, PA). The cochlear lateral wall from the basal turn was used for the study. Images were acquired with a x40 objective. A total of 60 images were recorded from five normal guinea pigs and a total of 80 images from seven noise-exposed guinea pigs were recorded. A total of 171 images were recorded from five normal mice and 65 images were recorded from three SU1498-treated normal mice. Seventy-five images were recorded from three sodium butyrate-treated animals. One hundred seventy-three images were recorded from seven noise-exposed mice. A total of 73 images were recorded from four noise-exposed mice treated with SU1498. Seventy-nine images were recorded from four noise-exposed mice treated with sodium butyrate and a total of ninety-one images were recorded from dimethyl sulfoxide (DMSO)-treated noise-exposed mice.
To determine pericyte coverage of endothelial cells (ECs), we labeled the pericytes with an antibody for desmin and labeled the ECs with isolectin GS-IB4 conjugated to Alexa Fluor 488 as described above. Images were analyzed for overlap of blood vessels and pericytes. For analysis, endothelium (red) and pericytes (green) were displayed and both sources of images were thresholded. A region tool in the Image J software was used to define and select the outer margin of the blood vessel under consideration. A delineated area in pixels and the percentage overlap (co-localization) of endothelium and pericytes was calculated. Pericyte coverage was quantified as a ratio of desmin-labeled area to isolectin-labeled area, in concurrence with a calculation method used by others.20
Evaluation of Immunostaining for VEGF
Immunostaining for VEGF was assessed and measured objectively by two independent observers (a senior researcher and a research assistant). VEGF labeling was measured with Image J (V1.38X) from a series of images obtained from the tissue segment. For each recorded image, the areas of the entire positive-labeled vessels were selected with a drawing tool, and the fluorescence intensity of the selected area was measured with the histogram function, obtaining a mean value. A background intensity was determined in a small window located away from the fluorescence of the vessels and was subtracted from the fluorescence intensity value from the vessels.9 Fluorescence was analyzed in 43 optical section images from control animals (n = 3), 87 optical section images from noise-exposed animals (n = 6), and 48 optical section images (n = 4) from sodium butyrate-treated noise-exposed animals.
SU1498 and Sodium Butyrate Treatments
To inhibit HIF-1 activity, mice were pretreated with an HIF-1 inhibitor, sodium butyrate (catalog no. 303410; Sigma-Aldrich, St. Louis, MO). Treatments were administered as acute, single-dose (intraperitoneal) injections (given in a 20-µl volume, 200 mg/kg, drugs were dissolved in saline as vehicle, Schroeder and colleagues,39
) 30 minutes before the animal received the noise exposure. For inhibition of VEGF activity, the mice were pretreated with SU1498 (T4192, Sigma-Aldrich) by one periocular injection (given in a 10-µl volume, SU1498 50 mg/kg) 30 minutes before noise exposure. DMSO vehicle was the control. Dosage of SU1498 was based on studies by Saishin and colleagues,40
and Cebulla and colleagues,41
who demonstrated effects on vessel function from the injection of VEGF in C57BL/6J mice.40,41
In addition, to determine whether either sodium butyrate or SU1498 has side-effects on auditory function, auditory brain-stem response was used to measure hearing threshold. We found that neither sodium butyrate nor SU1498 had ototoxicities in all six tested mice in the two treated groups (n = 3). To determine whether dosage of SU1498 was sufficient to inhibit VEGF activity, the activity of focal adhesion kinase, a widely expressed cytoplasmic protein tyrosine kinase involved in VEGF-mediated signal transduction42
has been examined in the cochlear lateral wall tissues. We found that immunoreactivity for focal adhesion kinase in the noise-exposed mice was high compared with the nonnoise exposed mice. A pretreatment with SU1489 to the noise-exposed mice significantly attenuated focal adhesion kinase immunoreactivity (data not shown).
Statistics and Analysis
Data are presented as means ± SD. Differences between data sets were assessed with Student’s t-test. n refers to the number of animals. Differences were considered significant at P < 0.05.
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Results |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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Both mice and guinea pigs had significant sensitivity loss after being exposed to intense noise at 120 dB SPL for 3 hours. Auditory brain-stem response thresholds at all test frequencies were increased (Figure 1
; nmouse = 5; nGP = 5; P < 0.001 at all test frequencies).
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The cochlea of the guinea pig has 
4 turns of cochlear lateral wall (Figure 2A)
and 1.5 turns in the mouse (Figure 2B)
. The locations of the rectangles in the right panels of Figure 2, A and B
, represent areas that we examined in the control and noise-exposed animals in this study. The stria vascularis, attached to the lateral wall fibrocyte (FC) layer, comprises marginal cells (MCs), intermediate cells (ICs), basal cells (BCs), and a dense capillary network. The capillary network in the stria vascularis (V/SV) lies between two cell layers connected by tight junctions (TJs); one is the epithelial MC layer, and the other is the mesodermal BC layer. Numerous basal infoldings of MCs and dendrite-like projections of ICs and BCs made close contact with the capillaries (Figure 2C)
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; MC/arrow, BC/arrow, IC/arrow, and V/arrow). Under high magnification, the microvascular wall comprises ECs surrounded by a pericyte (PC)/basal lamina (BL) in normal mice (Figure 2F)
and normal guinea pigs (Figure 2G)
. PCs were closely associated with ECs and their foot processes (fp/PC) tightly positioned next to the endothelium in normal mice (Figure 2F
, fp/PC/arrowheads) and guinea pigs (Figure 2G
, fp/PC/arrowheads). The permeability of the endothelium was normal because no immunofluorescence signals of serum protein IgG H+L were found outside of the capillaries of the stria vascularis in normal mice (Figure 2H
, IgG/arrow) and normal guinea pigs (Figure 2I
, IgG/arrow). In contrast, mice (Figure 2J)
and guinea pigs (Figure 2K)
exposed to wide-band noise at the level of 120 dB for 3 hours per day for 2 consecutive days had edema and swelling of the stria vascularis. Intercellular spaces between ECs, PCs, and ICs were enlarged in the stria vascularis in the mice (Figure 2J
, arrowheads) and guinea pig (Figure 2K
, arrowheads). Endothelial cell vacuolization was seen in a capillary of the stria vascularis in a noise-exposed mouse (Figure 2L
, arrow). A disrupted capillary wall was seen in a noise-exposed guinea pig (Figure 2M
, arrowheads). Serum protein IgG was found outside of vessels in the stria vascularis of the mice (Figure 2N
, arrowheads) and guinea pigs (Figure 2O
, arrowheads). Pericyte Coverage Was Significantly Increased in Both Mice and Guinea Pigs
Immunohistochemically labeled desmin enables pericytes to be visualized under fluorescence confocal microscopy. In a normal guinea pig and mouse ear, pericytes on the capillaries were oriented longitudinally along the vessels, with long thin foot processes, positioned next to the endothelium. We found pericyte coverage, as defined in the Materials and Methods, to be 12% in the guinea pig (Figure 3A
, examples of pericyte distributions on two vessels of stria vascularis) and 19% (Figure 3B
, an example of desmin distributions on vessels of the stria vascularis) in the mice. In contrast, when animals were exposed to wide-band noise at the level of 120 dB for 3 hours per day for 2 consecutive days, pericytes irregularly positioned along the vessel walls of the stria vascularis in both the guinea pigs (Figure 3D)
and mice (Figure 3E)
. Some pericyte foot processes were detached from the capillaries of the guinea pigs (Figure 3D
, arrow) and mice (Figure 3E
, arrows). Interestingly, pericyte coverage was substantially increased to 40% in the capillaries of the stria vascularis of the guinea pig (Figure 3G
; ncontrol = 5, nnoise = 7, **P < 0.01) and to 39% in the mice (Figure 3E
; ncontrol = 5, nnoise = 7, **P < 0.01).
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With Western blot analysis, we also found that the amount of desmin expression was increased in noise-exposed guinea pig whole cochlear lateral wall (Figure 4A)
and the stria vascularis of noise-exposed mice (Figure 4B)
after 2 consecutive days of noise exposure. The change of desmin protein expression measured by Western blot in guinea pigs appears to be greater in comparison with mice (Figure 4, A and B)
. This may be explained by the combined cell population of FCs, which also express desmin (unpublished data) and pericytes in the guinea pig lateral wall, whereas the mouse stria vascularis has only a single desmin-expressing cell population of pericytes. In contrast, desmin protein was not significantly up-regulated in both guinea pig retina and mouse retina (Figure 4, A and B)
. These findings indicate that pericyte desmin expression is a regional response to noise injury. With quantitative real-time PCR technique, the mRNA levels for Desmin in the cochlear lateral wall of the mice were also significantly increased after noise exposure to wide-band noise at the level of 120 dB for 30 minutes, 1 hour, and 3 hours per day for 2 consecutive days (Figure 4C
; n = 10, **P30 minutes < 0.01, **P60 minutes < 0.01, **P2 days < 0.01).
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Pericytes in the stria vascularis of normal mice, examined by electron microscopy, were tightly associated with ECs, with foot processes positioned next to the endothelium, as shown in Figure 2
. Under high magnification, a dense and continuous BL is seen between pericytes and ECs (Figure 5, A and B)
. In contrast, pericytes in noise-exposed mice had an abnormally loose association with ECs, with wide spaces separating some regions of the two types of cells (Figure 5, C and D)
. Basal lamina appeared less electron-dense (Figure 5D)
. Also the numbers of small pericytes found on the capillaries suggest they were proliferating (Figure 5C
, with arrows). Pericyte foot processes were migrating into the BL (Figure 5, E and F)
, and the size of pericyte foot processes was larger than in a normal animal (Figure 5G)
. The BL between pericytes and ECs was discontinuous in places. (Figure 5H)
. A similar abnormal morphological character was found in the guinea pigs (data not shown).
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Translocation in Mice
Insufficient blood supply induced by noise causes pO2 to drop and the cochlear environment to turn hypoxic.7,43
The transcriptional complex hypoxia-inducible factor 1 (HIF-1) plays a key role in the cellular adaptations to the lack of oxygen supply.44
In this study, using an immunohistochemical technique, the immunoreactivity of HIF-1 protein was examined under fluorescence confocal microscopy. We found no or little immunoreactivity to HIF-1 in various cells of the cochlear lateral wall in the control mice (Figure 6A)
. This finding was consistent with the notion that HIF-1 is degraded under normoxia.34
However, within as little as 30 minutes of noise exposure, HIF-1 immunoreactivity was increased in various cells including vascular and nonvascular cells in the stria vascularis and remained active for 2 days of noise exposure. With double labeling for HIF-1 and cell nuclei with propidium iodide, HIF-1 induction and translocation can be seen in the pericytes (Figure 6, B and C
; PC/arrow) and the ECs (Figure 6, B and C
; EC/arrow) as well as in nonvascular MCs (Figure 6, B and C
; MC/arrow) at as early as 30 minutes noise exposure and a prolonged noise exposure (Figure 6C)
. This can be better visualized from the high-magnification inset images from the location of the rectangle in Figure 6, B and C
. With quantitative real-time PCR, the mRNA levels for Hif-1 in the cochlear lateral wall were also significantly increased after noise exposure to wide-band noise at the level of 120 dB for 30 minutes, 1 hour, and 3 hours per day for 2 consecutive days (Figure 6D
; n = 3, **P30 minutes < 0.01; **P60 minutes < 0.01; *P2 days < 0.05).
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Activation in Mice
VEGF production can be induced in cells that are not receiving enough oxygen and then it can bind to VEGF receptors on vascular cells, triggering a tyrosine kinase pathway leading to vascular remodeling.42
We hypothesize that the alteration in pericyte coverage may involve VEGF up-regulation and HIF-1 regulatory mechanisms. In this experiment, with immunohistochemical technique, we found VEGF constitutively expressed in various cells in the stria vascularis, including both vascular cells (pericytes and ECs) and nonvascular cells such as MCs in the control mice (Figure 7A
; V/arrow, MC/arrow). In contrast, increased expression of VEGF was found in those cells in noise-exposed mice, (Figure 7B
; V/arrow, MC/arrow). However, in animals pretreated with a HIF-1 inhibitor, sodium butyrate (SB), the expression of VEGF was significantly reduced (Figure 7C
; V/arrow, MC/arrow). A significant statistical difference in expression of VEGF in the different groups was found for the VEGF expression shown in Figure 7D
(ncontrol = 3; nNE = 6; nNE+SB = 4; **P < 0.01). Consistent with the results from immunofluorescence label for VEGF, the amount of VEGF protein in the stria vascularis analyzed by Western blot in the control, noise-exposed (NE) and SB-treated noise-exposed (NE+SB) groups also had a significant difference (Figure 7E)
. With quantitative real-time PCR, the mRNA level for Vegf was found to be increased as early as 30 minutes of noise exposure and remained at an elevated level after prolonged noise exposures (Figure 7F
; n = 10, **P30 minutes < 0.01; **P60 minutes < 0.01; **P2 days < 0.01). In addition, as expected, mRNA for Vegf was significantly down-regulated when the noise-exposed mice were pretreated with a HIF-1.inhibitor, sodium butyrate (SB) (Figure 7F
; n = 10, *P2 days+SB < 0.05), suggesting that the mRNA for Vegf was regulated by HIF-1.
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and VEGF Activities in Mice
In this study, we determined whether increased pericyte coverage was related to HIF-1 induction and up-regulation of VEGF. The desmin coverage was assessed in normal mice (Figure 8A)
, noise-exposed mice (NE, Figure 8B
), DMSO vehicle-pretreated noise-exposed mice (NE+DMSO, Figure 8C
), SU1498-pretreated noise-exposed mice (NE+SU1498, Figure 8D
), and sodium butyrate (SB)-pretreated animals (NE+SB, Figure 8E
). We also assessed pericyte coverage in the stria vascularis of SU1498- and SB-treated normal (no noise exposure) mice as control groups (data not shown). We found that inhibition of either HIF-1 or VEGF activity could significantly suppress noise-induced increased pericytes coverage. Although SU1498 treatment did not completely reduce increased pericyte coverage, there was a significant difference between the nontreated noise-exposed animals and the SU1498-treated noise-exposed animals. SU1498 and SB treatment alone had no significant effect on pericyte coverage in nonnoise-exposed normal mice (Figure 8F
; ncontrol = 5; nnoise = 7; nNE+SU1498 = 4; nNE+SB = 4; **P < 0.01; *P < 0.05).
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Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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induction and up-regulation of VEGF. Noise Damages the Blood-Labyrinth Barrier and Induces Vascular Leakage
Sound trauma significantly impairs the cochlear blood–labyrinth barrier (CLB). Noise causes significant hearing loss, and associated effects include disruption of the CLB and increased vascular permeability.38,45
Changes in the ultrastructure can be seen with transmission electron microscopy. In normal animals, pericytes are closely associated with ECs, with their processes tightly positioned adjacent to the endothelium (Figure 2, F and G)
. The pericytes and ECs share BL and are tightly coupled (Figure 2, H and I)
. The endothelium is functionally intact, indicated by absence of immunofluorescence for serum protein IgG leaked from capillaries (Figure 2, J and K)
. By contrast, animals exposed to wide-band noise at the level of 120 dB 3 hours/day for 2 consecutive days showed edema in the stria vascularis and swelling of ECs. The intercellular spaces between the ECs and pericytes were enlarged. The cytoplasm of ECs had vacuoles. Pericytes were irregularly positioned along the vessel walls of the stria vascularis, with foot processes that split the BL into several sheets and appeared as electron-transparent zones (Figure 5F)
. Serum proteins IgG and albumin (data not shown for albumin) were found leaking from vessels in the stria vascularis (Figure 2, R and S)
.
Complex physical and biochemical sequelae are likely responsible for the disruption of the CBL. Cochlear pericytes are a major component of CLB structure, and abnormal interaction between the ECs and pericytes may be significantly contributing to the noise induced vascular leakage. In addition to the physical deterioration of the CLB, up-regulation of VEGF may also be increasing vascular permeability. VEGF is known to be a potent angiogenic and vascular permeability factor.46,47 Increased vascular permeability, induced by VEGF, is reported in different microvasculatures, including the lung.48,49 Our data are consistent with those of Picciotti and colleagues50 and Selivanova and colleagues,51 who found that noise stimulation significantly increases VEGF expression in the stria vascularis.50
Noise Increases Expression of Desmin in Pericytes
The major finding of this study is that noise trauma increases the expression of desmin, a structural protein, in pericytes. In addition, the numbers of pericytes on vessel walls is increased by noise. The results are robust, found in both guinea pig and mice.
Desmin is a muscle- and pericyte-specific protein. It is a key subunit of intermediate filaments in cardiac, skeletal, and smooth muscle, critical to the structural and mechanical integrity of the contractile apparatus in muscle tissues.52,53 Results from mice deficient in desmin reveal the fundamental role desmin filaments play in cell architecture and force transmission. Mice lacking desmin develop cardiomyopathy with increased heart weight.54
The cochlear microvasculature is a vascular network dense with pericytes.10
However, unlike pericytes on the capillaries of the cochlear spiral ligament and other organs, pericytes on the vessels of the stria vascularis do not express contractile proteins such as -SMA or tropomyosin. Instead, they are rich in desmin.10
Because pericytes are a major component of the vessel wall, desmin is thought to play a role integral to the mechanical strength of strial vessels.
Change in vascular architecture is known to be correlated with blood flow and vascular stability.55 Increased expression of desmin protein may be an adaptive strategy for coping with the physiological challenges imposed by loud sound. Higher internal pressure in capillaries may require more pericyte coverage to maintain vascular integrity.21 Blood flow in the stria vascularis is known to be slower than in the straight vessels of the spiral ligament,5 a flow configuration providing for effective exchange of oxygen and metabolites in cells such as MCs and ICs. High metabolic rates are needed to maintain the high-voltage endolymph potential.
Noise significantly impairs cochlear blood flow by vasoconstriction and blood stagnation in capillaries. Although it is unknown how this impairment occurs, mechanical strain, metabolic stress, or both are thought to be involved.7,56-58 Perturbation of blood flow caused by irregular vasomotion, such as overcontraction59 or dilation by a locally increased substrate of prostacyclin, NO, hemokines, and adhesion molecules,9,38 could change hydrostatic pressure and generate a pressure overload on the capillary networks. Shear forces on cells of the lateral wall during loud sound may also be important. Increased pericyte structural proteins may provide the physical strength that prevents the capillary network from deformation and loss of integrity under the forces of sound and osmotic pressure.
Increased pericyte coverage may also increase intercellular communication and material exchange between cells. Pericytes contact ICs through gap junctions. Pericytes have been shown to be dye-coupled to surrounding intermediate, basal, and capillary ECs.60 These coupled cells exchange ions, metabolites, and messengers through the gap junctions. Increased coverage may also be increasing signal transmission and interaction between ECs and ICs.
Disruption of normal endothelial-pericyte interactions may profoundly affect subsequent remodeling. The increased desmin may have a functional role in remodeling the blood-labyrinth barrier, particularly given the central role pericytes play in vascular stability, structural integrity, and angiogenesis in general. Pericytes prevent disruption of the endothelial barrier under hypoxic stress by contributing to cell-to-cell contacts and gap junctions between ECs.61
Involvement of HIF-1 and VEGF
Insufficient blood supply after noise exposure results in a drop in pO2 leaving a highly hypoxic environment in the cochlea.7,43,62
Induction of HIF-1 plays an essential role in triggering protective metabolic changes in response to oxygen deprivation. Up-regulation of VEGF expression in hypoxia is signaled through HIF-1.30,34
We found that HIF-1 translocation and VEGF up-regulation also play a mediary role in noise-induced damage. Suppressing HIF-1 activity with sodium butyrate, a histone deacetylase inhibitor,19,63
down-regulated VEGF at both the transcription and protein levels (Figure 7)
. Pretreatment with an inhibitor for the VEGF receptor significantly reduced desmin expression (Figure 8D)
. Direct inhibition of HIF-1 also significantly suppressed desmin expression (Figure 8E)
. Our data suggest that alteration in pericyte coverage involves HIF-1-induced VEGF regulatory mechanisms. Nonetheless, the specific mechanism involved in VEGF-related up-regulation of desmin remains unknown.
Noise increased the level of Hif-1, Vegf, and Desmin transcripts in the cochlear lateral wall. Transcripts for Vegf and Desmin were found as early as 30 minutes after exposure. This is consistent with the view that a hypoxic environment is induced in the cochlea by noise immediate with the sound trauma.6,32
However, the increased mRNA level measured may not be exclusively of vascular origin. Nonvascular cells in the cochlear lateral wall such as MCs, ICs, BCs, and FCs may also be contributing. A further study will be required to determine changes in mRNA to loud sound localized to specific cells. Nevertheless, real-time PCR data from whole lateral wall showing a significant increase in the three gene products serves as additional evidence of increased HIF-1 and VEGF activity.
These findings support a hypothesis that noise provokes a homeostatic reaction to a hypoxic perturbation in the stria vascularis. In the hypoxia condition, HIF-1 is less degraded and stabilized. Accumulated stabilized HIF-1 translocates to the nucleus and then binds to hypoxia-response elements in the promoters of many genes including the Vegf gene.64
Specially, HIF-1 binds to the 5'-flanking region of the Vegf gene and not only stabilizes mRNA Vegf, but also increases its transcription rate, in which VEGF can be significantly increased.64,65
Consequentially, increased VEGF mediates pericyte remodeling such as structural protein desmin up-regulation through unknown mechanisms. These concepts are summarized diagrammatically in the model shown in Figure 9
. The cellular pathways linking VEGF and the remodeling of pericytes dependent on vascular integrity, and specifically desmin expression, are yet to be explored.
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expression on noise exposure may be altering pericyte structure through VEGF up-regulation.
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Acknowledgements |
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Footnotes |
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Supported by the National Institute of Deafness and Other Communications Disorders (grants R01 DC000141, R03 DC008888-02, and P30 DC005983).
Accepted for publication January 27, 2009.
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References |
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