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Supplemental Figure 1 - A) Lysates of PNT1A, LNCaP and DU-145 were subjected to EphA2 immunoblot analyses. B) Boyden invasion assay. PNT1A, LNCap and DU-145 cells after 24 h of serum starvation, were seeded into the upper chamber of Boyden chambers pre-coated with a Matrigel 3D miliew and allowed to migrate for 24 h toward the lower chamber filled with complete growth medium. Cell invasion was evaluated after crystal violet staining and photographs are shown. C) DU-145 cells expressing kinase-deficient mutants, after 24 h of serum starvation, were seeded into the upper chamber of Boyden chambers and treated as in Fig 6C. The results are representative of four expirements. *P<0.001 stimulated vs control.

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