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Originally published online as doi:10.2353/ajpath.2007.070273 on September 20, 2007

Published online before print September 20, 2007
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(American Journal of Pathology. 2007;171:1529-1537.)
© 2007 American Society for Investigative Pathology
DOI: 10.2353/ajpath.2007.070273

Thy-1 Is Expressed in Hepatic Myofibroblasts and Not Oval Cells in Stem Cell-Mediated Liver Regeneration

Katalin Dezso*, Peter Jelnes{dagger}, Viktória László*, Kornélia Baghy*, Csaba Bödör*, Sándor Paku*, Niels Tygstrup{ddagger}, Hanne Cathrine Bisgaard{dagger} and Peter Nagy*

From the First Department of Pathology and Experimental Cancer Research,* Semmelweis University, Budapest, Hungary; the Department of Cellular and Molecular Medicine,{dagger} The Danish Stem Cell Research Centre, The Panum Institute, University of Copenhagen, Copenhagen, Denmark; and the Department of Hepatology,{ddagger} Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark

Thy-1, a marker of hematopoietic stem cells, has been reported to be expressed by oval cells proliferating during stem cell-mediated regeneration in rat liver, suggesting a relationship between the two cell populations. Consequently, Thy-1 has become an accepted cell surface marker to sort hepatic oval cells. In the present study we used the well-characterized 2-acetylaminfluorene/partial hepatectomy model to induce transit-amplification of hepatic oval cells in the regenerating liver and characterized Thy-1 expression using Northern hybridization, quantitative reverse transcriptase-polymerase chain reaction analysis, immunofluorescence confocal microscopy, and immunoelectronmicroscopy. We found that Thy-1 expression was induced during transit-amplification of the oval cell population, but Thy-1 mRNA was not present in the {alpha}-fetoprotein-expressing oval cells. Thy-1 protein was consistently present outside the basement membrane surrounding the oval cells. It overlapped frequently with smooth muscle actin staining. A similar cellular localization of the Thy-1 protein was found on human liver specimens with ductular reactions obtained from patients with fulminant liver failure. Furthermore, Thy-1 was expressed by myofibroblasts in experimental liver fibrosis models without oval cell proliferation. We conclude that Thy-1 is not a marker of oval cells but is present on a subpopulation of myofibroblasts/stellate cells.




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[Abstract] [Full Text] [PDF]


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