help button home button Am J Pathol PCR Enhanced. PCRboost from Biomatrica
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
A more recent version of this article appeared on December 1, 2007

Published online before print November 30, 2007
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
ajpath.2007.060857v1
171/6/2040    most recent
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by King, V. L.
Right arrow Articles by Daugherty, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by King, V. L.
Right arrow Articles by Daugherty, A.
Copyright © 2007 American Society for Investigative Pathology
American Journal of Pathology, doi:10.2353/ajpath.2007.060857

Accepted for publication August 24, 2007.

Article

Interleukin-4 Does Not Influence Development of Hypercholesterolemia or Angiotensin II-Induced Atherosclerotic Lesions in Mice

Victoria L. King*{dagger}@, Lisa A. Cassis*{dagger}, and Alan Daugherty*{dagger}

From the Cardiovascular Research Center,* and the Graduate Center for Nutritional Sciences,{dagger} University of Kentucky, Lexington, Kentucky

@ To whom correspondence should be addressed. E-mail: vicky.king{at}uky.edu.


  Abstract

Interleukin-4 (IL-4) has been detected in both human and mouse atherosclerotic lesions, although its effects on the development of the disease are undefined. We determined the role of IL-4 in the most commonly used murine models of atherosclerosis by defining the effects of exogenous delivery and genetic deficiency of this cytokine on both hypercholesterolemia and AngII-induced atherosclerosis in apolipoprotein E (apoE)-/- mice and different dietary stimuli in low-density lipoprotein (LDL) receptor-/- mice. Exogenous administration of IL-4 (1.1 ng g-1 day-1 i.p. for 30 days) into female apoE-/- mice had no effect on lesion size or composition in mice fed normal or saturated fat diets. Also, IL-4 deficiency had no significant effect on the size or composition of atherosclerotic lesions in two vascular areas of male and female apoE-/- mice fed either a normal or saturated fat diet. IL-4 deficiency was also studied in age-matched male mice infused with AngII (1000 ng kg-1 min-1) for 28 days. Whereas AngII infusion augmented atherosclerotic lesion formation, IL-4 deficiency did not influence atherosclerotic lesion size or composition. Finally, different dietary stimuli also had no effect on atherosclerotic lesion size in female LDL receptor-/- mice. These data demonstrate that IL-4 does not significantly influence the development of atherosclerotic lesions in apoE-/- mice of either gender or in female LDL receptor-/- mice, irrespective of the mode of induction of atherosclerosis.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2007 by the American Society for Investigative Pathology.