Materials and Methods
Mouse and Tissue Samples
Tissue Preparation Procedures for LS-TAFI
Application of LS-TAFI to Three-Dimensionally Visualize Mouse Mammary Gland Whole Mounts
Application of LS-TAFI to Three-Dimensionally Visualize Pathological Tissue
Application of LS-TAFI to Visualize Whole-Mount Lung, Pancreas, and Embryo
Application of LS-TAFI to Visualize Mammary Gland Whole Mount with β-Gal Staining
- Supplemental Figure S1
Images of terminal end bud from two different mouse strains at age 5 weeks. Carmine-stained mammary gland whole mount of SPRET/EiJ and Balb/cJ are shown. To detect fluorescence signal effectively, MBS 561 nm, DB 630–740 nm, and 40× objective lens were used. Scale bar = 20 μm. Lumen (asterisk), body cells (white arrowhead), cap cells (blue arrowhead), stromal cells (yellow arrowhead), and adipocytes (green arrowhead) are indicated.
- Supplemental Movie S1
Z-stack of LS-TAFI–scanned mammary gland ductal tree of Figure 1E.
- Supplemental Movie S2
Z-stack of LS-TAFI–scanned mammary gland terminal end bud of Figure 1G.
- Supplemental Movie S3
Z-stack of LS-TAFI–scanned mammary gland terminal end bud of Figure 1I.
- Supplemental Movie S4
Z-stack of LS-TAFI–scanned blood veins of Figure 1J.
- Supplemental Movie S5
Z-stack of LS-TAFI–scanned mammary tumor nodules of Figure 2C.
- Supplemental Movie S6
Z-stack of LS-TAFI–scanned lung of Figure 3A.
- Supplemental Movie S7
Z-stack of LS-TAFI–scanned pancreas of Figure 3C.
- Supplemental Movie S8
Z-stack of LS-TAFI–scanned embryo of Figure 3E.
- Supplemental Movie S9
Z-stack of LS-TAFI–scanned mammary gland terminal end bud with β-gal staining of Figure 4D.
- Visualization of image data from cells to organisms.Nat Methods. 2010; 7: S26-S41
- Crosstalk between neovessels and mural cells directs the site-specific expression of MT1-MMP to endothelial tip cells.J Cell Sci. 2007; 120: 1607-1614
- Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy.Mem Inst Oswaldo Cruz. 1998; 93: 303-307
- Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland.Dev Dyn. 2006; 235: 3222-3229
- Stromal effects on mammary gland development and breast cancer.Science. 2002; 296: 1046-1049
- Distinct genetic loci control development of benign and malignant skin tumours in mice.Nat Genet. 1995; 10: 424-429
- Genetic architecture of mouse skin inflammation and tumour susceptibility.Nature. 2009; 458: 505-508
- Comparison of mouse mammary gland imaging techniques and applications: reflectance confocal microscopy.BMC Cancer. 2008; 8: 21
- Cell and tissue autofluorescence research and diagnostic applications.Biotechnol Annu Rev. 2005; 11: 227-256
- Quantitative optical spectroscopy for tissue diagnosis.Annu Rev Phys Chem. 1996; 47: 555-606
- A biomarker that identifies senescent human cells in culture and in aging skin in vivo.Proc Natl Acad Sci USA. 1995; 92: 9363-9367
- Using reporter genes to label selected neuronal populations in transgenic mice for gene promoter, anatomical, and physiological studies.Prog Neurobiol. 2001; 63: 673-686
- Two-photon laser scanning fluorescence microscopy.Science. 1990; 248: 73-76
- Deep tissue two-photon microscopy.Nat Methods. 2005; 2: 932-940
- Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy.Science. 2008; 322: 1857-1861
- Imaging chromophores with undetectable fluorescence by stimulated emission microscopy.Nature. 2009; 461: 1105-1109
Supported by grants from the U.S. Department of Energy, Office of Biological and Environmental Research and Low Dose Radiation Program (contract no. DE-AC02-05CH1123 ), the National Cancer Institute (awards R37CA064786 , U54CA126552 , R01CA057621 , U54CA112970 , U01CA143233 , and U54CA143836 - Bay Area Physical Sciences–Oncology Center, University of California, Berkeley, California), the U.S. Department of Defense ( W81XWH0810736 ), the Susan G. Komen Breast Cancer Foundation ( 02-1591 to H.M, KG111229 to R.B), the NIH National Center for Research Resources ( K26 RR024037 to A.D.B.), the NIH National Cancer Institute ( U01 CA14582 to A.D.B.), a Glenn T. Seaborg postdoctoral fellowship from Lawrence Berkeley National Laboratory (C.M.G.).
User LicenseCreative Commons Attribution – NonCommercial – NoDerivs (CC BY-NC-ND 4.0) |
For non-commercial purposes:
- Read, print & download
- Redistribute or republish the final article
- Text & data mine
- Translate the article (private use only, not for distribution)
- Reuse portions or extracts from the article in other works
- Sell or re-use for commercial purposes
- Distribute translations or adaptations of the article
Elsevier's open access license policy